Post

@brainevodevo.bsky.social

1/ Our new study, led by Jingwen Ding, examines the role of transcription factors during human neurogenesis to identify gene regulatory networks influencing cell fate, maturation, and subtype specification www.nature.com/articles/s41...

nature.comDissecting gene regulatory networks governing human cortical cell fate - NatureSystematic screening of transcription factors reveals conserved mechanisms governing cortical radial glia lineage progression across primates and provides a framework for functional dissecti...

23/1/26, 1:16 am28 reposts68 likes

Keri Martinowich

@martinowk.bsky.social

23 Jan

Congratulations Alex to you and your team 🤩

Cedric Boeckx

@cedricboeckx.bsky.social

23 Jan

Congrats to the whole team 👏👏

Alex Pollen

@brainevodevo.bsky.social

23 Jan

2/ Radial glia are multipotent progenitor cells that give rise to most cells in the cortex, including excitatory neurons, oligodendrocytes and astrocytes, as well as olfactory bulb interneurons and a recently characterized population of cortical interneurons.

Alex Pollen

@brainevodevo.bsky.social

23 Jan

3/ We used CRISPRi to repress 44 transcription factors in human radial glia and then measured the consequences on neurogenesis using single cell RNA sequencing.

Alex Pollen

@brainevodevo.bsky.social

23 Jan

4/ We identified multiple TFs with new roles in cortical neurogenesis, including ZNF219 that represses neural differentiation.

Alex Pollen

@brainevodevo.bsky.social

23 Jan

5/ We also identified NR2E1 and ARX that have opposing roles in regulating the balance of excitatory versus inhibitory neurogenesis. By combining single cell lineage tracing with Perturb-seq, we discovered their roles in balancing RG lineage plasticity and progression.

Beverly Piggott

@beverly-piggott.bsky.social

23 Jan

Congratulations Alex to you and your team. Sounds like a fantastic story. I look forward to digging into it!

Jeremiah Tsyporin

@tsyporin.bsky.social

24 Jan

Beautiful work, congrats!!

Indigo V. L. Rose

@ivlrose.bsky.social

23 Jan

You missed the best part: that the data in this paper was so powerful that when Jingwen presented it in your lab meeting there was literally an earthquake!!