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Bussi Lab
@bussilab.org

The alx aptamer uniquely integrates two orthogonal signals — Mn²⁺ and cytoplasmic pH — to control gene expression.

Bussi Lab
@bussilab.org

Using conventional and constant-pH #MD simulations, we identified a pH-dependent alternative base-pairing interaction in a loop that allosterically perturbs the Mn²⁺-binding site and remodels the conformational ensemble.

Bussi Lab
@bussilab.org

These models complement single-molecule #FRET experiments revealing pH-tuned conformational sampling, as well as functional in vivo assays showing that both the Mn²⁺-binding core and the pH-sensitive loop are required for maximal activation.

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Bussi Lab
@bussilab.org

Together, the work defines how an RNA #aptamer integrates metal and proton cues for combinatorial environmental sensing.