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Chris Bahl
@cdbahl.com

We always strive for our work to substantially benefit the greater scientific community, and it's such a thrill every time it happens. A massive thank you to @addgene.bsky.social for amplifying the impact of researchers everywhere! 🧵👇

Chris Bahl
@cdbahl.com

This Blue Flame Award is for a plasmid encoding an improved SUMO protease enzyme, and it was the work of an amazing team: Kara Lau, Vladimir Baytshtok, Tessa Howard, Brooke Nickerson, JayLee Johnson, Lauren Carter, David Baker and Chris Lima www.jbc.org/article/S002...

jbc.orgDiscovery and engineering of enhanced SUMO protease enzymesSmall ubiquitin-like modifier (SUMO) is commonly used as a protein fusion domain to facilitate expression and purification of recombinant proteins, and a SUMO-specific protease is then used to remove ...
Chris Bahl
@cdbahl.com

If you found this SUMO protease helpful, check out our latest publication which builds on it and makes it even better: onlinelibrary.wiley.com/doi/10.1002/... Hopefully another 100+ research labs around the world will benefit!

onlinelibrary.wiley.comFacile, high‐throughput protein purification enabled by high‐efficiency protease elutionWhile advances in artificial intelligence have made protein design widely accessible, protein production and characterization remain a bottleneck. Protease-cleavable affinity tags are commonly used t...

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