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Cell Reports Methods

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Strategic enhancement of Cas9 nickase-driven gene amplification

dlvr.itStrategic enhancement of Cas9 nickase-driven gene amplificationTakesue et al. enhance the nCas9-based gene amplification method BITREx through the development of (1) a plasmid reporter that predicts gRNA performance and (2) BITREx 2.0, a dual-nicking strategy that exploits synergistic interactions between two gRNAs. These advances broaden the utility of BITREx for gene amplification applications.

6/8/26, 6:49 pm0 reposts0 likes

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