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🔑Key findings: 🌐Plants use structural and chemical barriers not only to block pathogens but also to fine-tune beneficial partnerships. 🌐In 15 Arabidopsis mutants, some defense barriers reduced or enhanced WCS417-driven growth promotion and root colonization.

🌐Root exudates from different mutants altered WCS417 transcriptome. Camalexin strongly inhibited bacterial motility/chemotaxis. 🌐On the 🌿side, WCS417 triggered distinct transcriptome/ROS responses depending on defense barrier. 🌐Overall, root defense barriers help plants balance growth and defense.

🌍 Why it matters: These insights extend the known role of plant defenses beyond pathogen resistance — showing they are also crucial in managing beneficial microbiome interactions. This could guide new microbe-based strategies for sustainable agriculture.

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