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Merry Christmas! Our newest review of #singlemolecule #orientation localization microscopy is now online in #qrb. doi.org/10.1017/S003... We hope it is useful to users and technologists alike! @washu.bsky.social #WashU
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Top-right image is from Ezra and the Lee lab's beautiful POLCAM work of actin fiber networks. The POLCAM software is much easier to use than full PSF fitting: doi.org/10.1038/s415...
doi.orgPOLCAM: instant molecular orientation microscopy for the life sciences - Nature MethodsCombining localization and polarization microscopy can yield detailed insights into subcellular structures. POLCAM uses a polarization camera and wide-field microscopy for rapid measurement of super-r...
The bottom image is from our multi-view microscope work, showing amyloid aggregate infiltration of a spherical lipid bilayer: doi.org/10.1038/s415...
doi.orgSix-dimensional single-molecule imaging with isotropic resolution using a multi-view reflector microscope - Nature PhotonicsA multi-view reflector microscope based on polarization modulation and pupil splitting enables single-molecule orientation-localization microscopy with precisions of 10.9 nm and 2.0°.