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@triggerloop.bsky.social

Two extremely cool new papers on how AID gets targeted to appropriate genes for somatic hypermutation: paper 1 from Schatz lab at Yale shows that NELF and elongation components of the SEC are required- and suggests NELF can travel into genes /1 www.nature.com/articles/s41...

nature.comTranscription pause and elongation regulators mediate somatic hypermutation - NatureThe molecular factors and mechanisms that mediate the chromatin recruitment of activation-induced deaminase and the generation of its stalled polymerase II substrate during somatic hypermutation are i...

30/9/26, 8:33 pm9 reposts24 likes

Craig Kaplan

@triggerloop.bsky.social

Potentially through the poised conformation identified by @voslab.org 2/ www.sciencedirect.com/science/arti...

sciencedirect.comDistinct negative elongation factor conformations regulate RNA polymerase II promoter-proximal pausingMetazoan gene expression regulation involves pausing of RNA polymerase (Pol II) in the promoter-proximal region of genes and is stabilized by DSIF and…

Craig Kaplan

@triggerloop.bsky.social

The second paper is from Di Noia lab at ICRM in Montreal, also describing the role of SEC proteins and histone readers MLLT1 and 3 www.nature.com/articles/s41...

nature.comHistone readers MLLT1 and MLLT3 concentrate AID to confer locus specificity - NatureBy being restricted to target regions by MLLT1–MLLT3 occupancy, activation-induced deaminase activity is able to avoid most off-target mutagenesis during B cell somatic hypermutation.