Chromatin labeling and imaging group

Chromatin labeling and imaging group

@andimaging.bsky.social

MPI for Multidisciplinary Sciences Fluorescent #probes for live-cell #nanoscopy: #STED, #MINFLUX and #confocal. We do the job, all of you judge if it is useful!

803 followers2.2K following310 posts

Chromatin labeling and imaging group reposted

Marie Auvray
@mauvray.bsky.social

Thrilled to share with you our work on covalent probes! By introducing a biocompatible sulfonium-based cleavable linker, we developed a series of fluorescent probes that can label covalently endogenous tubulin in various cell lines. www.biorxiv.org/content/10.1... #Chembio #FluorescenceFriday

Chromatin labeling and imaging group
@andimaging.bsky.social

We have introduced a new biocompatible sulfonium-based building block for generation of non-cytotoxic covalent fluorescent probes. The proof-of-concept paper is on BioRxiv: https://doi.org/10.1101/2025.01.27.635008 . Great job done by @MarieAuvray4 at @mpi_nat ! Enjoy reading!

doi.orgBiocompatible sulfonium-based covalent probes for endogenous tubulin fluorescence nanoscopy in live and fixed cellsFluorescent probes enable the visualization of dynamic cellular processes with high precision, particularly when coupled with super-resolution imaging techniques that surpass the diffraction limit. Traditional methods include fluorescent protein fusion (e.g., GFP) or organic fluorophores linked to ligands targeting the protein of interest. However, these approaches often introduce functional disruptions or ligand-associated biological effects. Herein, we address these challenges by developing covalent fluorescent probes for endogenous tubulin, a critical cytoskeletal protein involved in processes such as cell movement, division, and biomolecule trafficking. Using well-known tubulin binder cabazitaxel and cell permeable fluorophore silicon-rhodamine—as a basis, we introduce a novel biocompatible cleavable linker containing a sulfonium center. This allowed the construction of the optimized probe , 6-SiR- o -C9-CTX , demonstrating excellent cell permeability, fluorogenic properties, and the ability to covalently label tubulin across various human cell lines. Importantly, the targeting moiety could be washed out while preserving tubulin staining, ensuring minimal disruption of tubulin function. This labeling technique is compatible with STED nanoscopy in both live and fixed cells, offering a powerful high-resolution imaging tool. ### Competing Interest Statement G.L. is a co-inventor on the patent (EP2748173B1 and US9346957B2, applicant EPFL) describing SiR and its derivatives.
Chromatin labeling and imaging group
@andimaging.bsky.social

KTU alumnės chemikės kelias: nuo Marijampolės iki laboratorijos Vokietijoje, kurios departamento vadovas – Nobelio premijos laureatas https://ctf.ktu.edu/news/ktu-alumnes-chemikes-kelias-nuo-marijampoles-iki-laboratorijos-vokietijoje-kuriai-vadovauja-nobelio-premijos-laureatas #ktu

ctf.ktu.eduKTU alumnės chemikės kelias: nuo Marijampolės iki laboratorijos Vokietijoje, kurios departamento vadovas – Nobelio premijos laureatasKTU alumnė dabar studijuoja Makso Planko institute Vokietijoje, o jos departamento vadovas – Nobelio premijos laureatas.
Chromatin labeling and imaging group
@andimaging.bsky.social

All probes have to be used with caution. The improved design stains DNA brighter and is less toxic: https://doi.org/10.1039/C8SC05082A https://x.com/LSAjournal/status/1704457687100981675

doi.orgRhodamine–Hoechst positional isomers for highly efficient staining of heterochromatinHoechst conjugates to fluorescent dyes are popular DNA stains for live-cell imaging, but the relationship between their structure and performance remains elusive. This study of carboxyrhodamine–Hoechst 33258 conjugates reveals that a minimal change in the attachment point of the dye has dramatic effects on t
Chromatin labeling and imaging group
@andimaging.bsky.social

We enjoy microscopy of living cells stained with rhodamine's 4-isomers. Follow the dynamics of mitochondria (magenta), vimentin (green), tubulin (gray) and nucleus (blue). Need more information, read: https://doi.org/10.1038/s41467-023-36913-2 Happy #FluorescenceFriday and #StPatrickDay !

doi.orgA general highly efficient synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy - Nature CommunicationsRhodamines are privileged fluorescent dyes for labelling intracellular structures in living cells. Here, the authors present a facile protecting-group-free synthesis permitting generation of a wide range of symmetrical and unsymmetrical 4-carboxyrhodamines covering the whole visible spectrum.
Chromatin labeling and imaging group
@andimaging.bsky.social

Label size is becoming an important issue in modern #fluorescence #microscopy or #nanoscopy. See size comparison of the commonly used tags and labeling approaches - antibody, GFP, Halo-tag, SNAP-tag, nanobody or small probe (5-610CP-Hoechst). Happy #FluorescenceFriday! #SciArt

Chromatin labeling and imaging group
@andimaging.bsky.social

Planing or starting live-cell imaging experiments? You can find some guidance in the book chapter: Live-Cell Imaging: A Balancing Act Between Speed, Sensitivity, and Resolution https://link.springer.com/chapter/10.1007/978-3-031-04477-9_6

link.springer.comLive-Cell Imaging: A Balancing Act Between Speed, Sensitivity, and ResolutionLive-cell imaging is perhaps one of the most exciting and challenging activities in the field of microscopy. It is exciting as recent developments in microscope technology have enabled scientists to visualize cellular and subcellular processes in real time down to...