Basler Lab

Basler Lab

@basler-lab.bsky.social

Unraveling the secrets of bacterial injection needles .. (among other things)

236 followers1 following27 posts

Basler Lab
@basler-lab.bsky.social

When Pseudomonas is attacked, it strikes back! Outer membrane damage triggers a tit-for-tat T6SS retaliation via TagQ/TagR protein complex assembly, activating the PpkA kinase. Congrats to Maxim & team for uncovering this fascinating mechanism! www.nature.com/articles/s41...

nature.comMembrane damage induces TagQ/TagR complex-mediated H1-T6SS assembly in Pseudomonas aeruginosa - Nature CommunicationsThe H1-T6SS of Pseudomonas aeruginosa assembles in response to membrane damage. Here, the authors show that this response mechanism is regulated by the formation of a complex between the membrane lipo...
Basler Lab
@basler-lab.bsky.social

In our new paper, we uncover how Burkholderia switches from antibacterial T6SS-1 to eukaryotic-targeting T6SS-5. Surprisingly, actively turning off T6SS-1 assembly prevents mitochondrial damage and apoptosis. Congrats @christy-chc.bsky.social and the team! link.springer.com/article/10.1...

link.springer.comRegulation of Type VI Secretion Systems of Burkholderia during transition to intracellular lifestyle - EMBO ReportsPathogenic bacteria tightly regulate gene expression in response to environmental cues. In Burkholderia species, the Type VI Secretion Systems (T6SS) mediate interbacterial competition and host cell i...

Basler Lab reposted

Alejandro Tejada Arranz
@atejadaarranz.bsky.social

Have a look at our latest review on intracellular P. aeruginosa populations! @basler-lab.bsky.social @biozentrum.unibas.ch @nccr-antiresist.bsky.social

Trends in Microbiology@cp-trendsmicrobiol.bsky.social

Intracellular Pseudomonas aeruginosa: internalization and survival

dlvr.itIntracellular Pseudomonas aeruginosa: internalization and survivalPseudomonas aeruginosa is an opportunistic human pathogen that can colonize multiple tissues and establish chronic infections with a high rate of relapse. P. aeruginosa survives intracellularly in multiple cell types, including in biopsies from human patients. Intracellular bacteria are partially protected from the immune system, from antibiotics with poor penetration across eukaryotic membranes, and, in some cases, also from antibiotics that penetrate eukaryotic membranes, such as fluoroquinolones. This suggests that P. aeruginosa can establish an intracellular reservoir that may contribute to chronic infection and relapse. However, the intracellular life cycle of P. aeruginosa is poorly understood. In this review, we summarize the ways in which P. aeruginosa interacts with eukaryotic cells and the role of its protein secretion machineries in establishing an intracellular niche.
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Basler Lab
@basler-lab.bsky.social

🏃‍♂️ Steps unlock Defender growth & boost Prey cell armor. 🫀 Heart Rate spikes your Defenders' retaliation chance. 🔋 Body Battery dictates how fast cells can replicate. 🤯 Stress Levels trigger chaotic friendly-fire "brawls." ☀️ Solar Intensity acts as UV hazard to the grid.

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Basler Lab
@basler-lab.bsky.social

Big congrats to @atejadaarranz.bsky.social and co-authors on this paper showing novel insights into the resistance to #T6SS effectors and an unexpected link to antibiotic resistance! Thanks @nccr-antiresist.bsky.social for funding and supporting the collaboration with the Jenal lab! rdcu.be/eShqI

rdcu.beMechanisms of Pseudomonas aeruginosa resistance to type VI secretion system attacksNature Communications - Pseudomonas aeruginosa H1-T6SS delivers effectors in response to exogenous T6SS attacks. In this study, a CRISPRi screen identifies pathways involved in P. aeruginosa...
Basler Lab
@basler-lab.bsky.social

Happy to share our latest T6SS review: nature.com/articles/s41... Thank you, Jan, Danny (@dannyjamesward.bsky.social), Joana (@joanampereira.bsky.social), as well as reviewers and editors @natrevmicro.nature.com!

nature.comThe type VI secretion system and associated effector proteins - Nature Reviews MicrobiologyIn this Review, Basler and colleagues examine the type VI secretion system (T6SS), focusing on the diversity of antibacterial T6SS effectors and the evolutionary forces that shape them. They explain h...
Basler Lab
@basler-lab.bsky.social

The prey (blue) multiply, don't move and produce capsule (purple). The predators (red) move towards prey and attack non-kin. They divide only if they lyse prey. The defenders (orange) move randomly and defend themselves by retaliation (divide if they lyse a cell). Who wins?

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