Daniel Cazares

academic.oup.comEco-evolutionary responses to plasmid-dependent phage constrain the spread of multidrug-resistance plasmidsAbstract. Phage therapy offers an alternative to antibiotics for treating multidrug-resistant infections. Plasmid-dependent phages (PDPs) are promising the

3 June

Phage constrain multidrug resistance spread #Phage #Plasmid #AMR #EvolutionaryBiology #CraigMacLeanLab The final version of our paper is out in @isme-microbes.bsky.social academic.oup.com/ismej/articl... bsky.app/profile/dani... More exciting phage and plasmid research coming soon!

Daniel Cazares@danielcazares.bsky.social

Happy to share that our latest research on the eco-evolutionary dynamics of MDR plasmids and PDPs is now out (open access vAuthor) in The ISME Journal doi.org/10.1093/isme... Here, we explored how plasmid-dependent phages (PDPs) act as a selective pressure against the spread of multidrug resistance

doi.orgEco-evolutionary responses to plasmid-dependent phage constrain the spread of multidrug resistance plasmidsAbstract. Phage therapy offers a promising alternative to antibiotics for treating multidrug-resistant infections. Plasmid-dependent phages (PDPs) are part

Daniel Cazares reposted

Thomas Ipoutcha

@tomipou.bsky.social

16 Sept

Finally out! 🎉 After 3 years of postdoc in London, here is my main work, now published in Nature Comm. (and as co-corresponding author!) Using a combination of bioinformatic and experimental approaches, we propose a model of plasmid evolution in Staphylococcus. www.nature.com/articles/s41... ⬇️ 1/4

nature.comMobile genetic elements drive a plasmid fusion and deletion lifecycle shaping evolution and antimicrobial resistance - Nature CommunicationsPlasmids drive bacterial adaptation, but how their diversity arises has remained unclear. Here, they show that in Staphylococcus aureus, mobile genetic elements repeatedly fuse and delete plasmids, re...

Daniel Cazares reposted

Adam Mulkern

@mulkern.bsky.social

12 Aug

Out now in @natcomms.nature.com: how do bacteria evolve resistance to programmable antisense antibiotics? www.nature.com/articles/s41...

nature.comA systematic identification of resistance determinants to antisense antibiotics suggests adaptation strategies dependent on the delivery peptide - Nature CommunicationsIn this study, authors investigate the evolution of bacterial resistance to antisense peptide nucleic acid (PNA) antibiotics. They observe that this typically occurs mainly by reducing drug uptake via...

Daniel Cazares reposted

Craig MacLean

@craigmaclean.bsky.social

27 June

Replying to @craigmaclean.bsky.social

Projects working with Helen Alexander www.pnas.org/doi/10.1073/... and Raf www.nature.com/articles/s41... inspired me to think about this problem and I look forward to hearing your thoughts #microsky PS: I also don't think that heteroresistance is real

pnas.orgStochastic bacterial population dynamics restrict the establishment of antibiotic resistance from single cells | PNASA better understanding of how antibiotic exposure impacts the evolution of resistance in bacterial populations is crucial for designing more sustai...

Daniel Cazares reposted

Craig MacLean

@craigmaclean.bsky.social

27 June

New #microsky#AMR Perspective paper with @penamiller.bsky.social rdcu.be/fqBJo ANTIBIOTIC RESISTANCE (IE MIC) AND TOLERANCE ARE NOT BACTERIAL TRAITS! Please read on if you want an explanation for this provocative claim.

rdcu.beBeyond resistance and tolerance: rethinking evolutionary responses to antibiotics from the perspective of individual bacterial cellsNature Communications - Beyond resistance and tolerance: rethinking evolutionary responses to antibiotics from the perspective of individual bacterial cells

Daniel Cazares reposted

Kayla King, Ph.D.

@kayla-king.bsky.social

24 June

Evolutionary rescue in a phage community! New paper from former PhD student, Sam Greenrod, with brilliant colleague @craigmaclean.bsky.social : academic.oup.com/ismej/advanc...

academic.oup.comRapid adaptation accelerates competitive suppression in a parasite communityAbstract. Environmental stress leads to changes in community composition by altering competitive hierarchies and pushing taxa towards extinction. Parasites

Daniel Cazares reposted

Sofya Garushyants

@garushyants.bsky.social

12 June

Our paper on the YprA family of helicases and their roles in bacterial defence is now out! We describe ARMADA, a defence system synergise with Druantia and show that both systems spread horizontally on a novel type of mobile genetic element that we call SPIDERs www.sciencedirect.com/science/arti...

sciencedirect.comYprA-family helicases provide the missing link between diverse prokaryotic immune systemsBacteria and archaea possess an enormous variety of antiviral immune systems that often share homologous proteins and domains. YprA-family helicases a…

Daniel Cazares reposted

Caro Kobras 🐍

@carokobras.bsky.social

14 June

New paper 🧪🧬🧫GeneScanner is our new tool to profile genetic variation in bacterial populations. Use via GitHub or @pubmlst.org. Thanks to Ko and Pri @sheppardlab.bsky.social @broncio.bsky.social @kjolley.bsky.social @microbiologysociety.org @imibirmingham.bsky.social @ineosoxford.bsky.social

microbiologyresearch.orgGeneScanner: profiling genetic variation across bacterial populationsRapid, low-cost genome sequencing has transformed microbiology, advancing efforts to link genetic and phenotypic variation across diverse bacterial systems. Laboratory functional screens now uncover c...

Daniel Cazares reposted

Julia Frunzke

@frunzkelab.bsky.social

4 June

Now out in @pnas.org 🥳 "DNA-intercalating antiphage molecules trigger abortive infection through mutual destruction and synergize with bacterial immunity" --> bad timing can kill you, even as a virus. www.pnas.org/doi/10.1073/... @spp2330.bsky.social, @mibinet.bsky.social, @dfg.de

pnas.orgPNASProceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Julia Frunzke@frunzkelab.bsky.social

Happy to share our recent preprint: "DNA-intercalating antiphage molecules trigger abortive infection through mutual destruction and synergize with bacterial immunity" www.biorxiv.org/content/10.6... @spp2330.bsky.social, @mibinet.bsky.social, @dfg.de @hhu.de @fzj.bsky.social

Daniel Cazares reposted

Craig MacLean

@craigmaclean.bsky.social

3 June

Excited to share this new paper from the lab: academic.oup.com/ismej/articl...! See thread below

Daniel Cazares reposted

Paloma Rodera

@palomarodera.bsky.social

19 May

New paper out! 🔈 Genomic Characterization of the RyC collection: 50 Multidrug Resistant Clinical Isolates of Escherichia coli and Klebsiella spp. 50 MDR gut isolates, 2 sequencing platforms, 4 “omes,” and 1 mission: provide a resource to decode AMR and MGE dynamics www.biorxiv.org/content/10.6...

biorxiv.orgbiorxiv.org

A huge thank you to our brilliant collaborators at The Department of Biology @biology.ox.ac.uk University of Oxford @ox.ac.uk Sanger @sangerinstitute.bsky.social @cazares-adr.bsky.social @wfigueroac3.bsky.social and The UoM @brockhurstlab.bsky.social #Phage #Plasmid #AMR #EvolutionaryBiology

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• Evolutionary Constraints: These findings suggest an inherent trade-off between horizontal gene transfer and phage resistance that plasmids cannot easily circumvent

Our results highlight that targeting MDR plasmids with PDPs is a viable strategy to drive the loss of conjugation, effectively limiting the spread of antibiotic resistance in microbial communities

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• The Resistance Trade-off: Phage resistance was consistently linked to plasmid loss or reduced conjugation, though the effects were heterogeneous across different mutation profiles

• Molecular Signatures: We identified a spectrum of resistance phenotypes—from partial to complete—driven by targeted mutations and IS-mediated inactivation of conjugation genes.