Exciting Instruments

Your molecules are doing something exciting, let's find out what! We’re visiting labs and conferences across Europe and Boston over the next few weeks. Come meet us 👇

15 Sept

Find us in Europe this September: 16: Cambridge — Next Generation Biophysics 17–18: Frankfurt — GBM Compact 19–26: Iseo — Nanochemistry Camp 21: Paris — Institut Pasteur 21–25: Tutzing — Single-Molecule FRET 29 Sep–2 Oct: Murnau — 10th Murnau Conference

15 Sept

Boston researchers: Jake Isaacs, Mahmoud Abdelhamid and Robert Bell are available for meetings from 28 September–6 October. Have a system you’d like to discuss? Tell us what you’re working on and let’s arrange a chat: excitinginstruments.com/contact-us/

excitinginstruments.comIn-solution dynamics for mechanistic insights | Exciting InstrumentsDiscover how in-solution single-molecule dynamics add mechanistic insights across nutrient sensing, viral replication, and ternary complexes.

12 Aug

#Biology isn’t static. 🧬 Discover how #smFRET, #FCS and #FCCS reveal in-solution dynamics - from conformational changes and drug mechanisms to ternary complex cooperativity and stability. See how dynamic measurements turn molecular snapshots into mechanistic insight > buff.ly/vvoGpwu

10 Aug

👀 Strong binding, but no degradation? Join Dr Stefan Mueller on 20 Aug to learn how FCCS on the EI-FLEX Pro HT reveals #ternarycomplex formation and #cooperativity - key insights for #PROTACs and #molecularglues. Register: us06web.zoom.us/meeting/regi... #drugdiscovery #targetedproteindegradation

6 Aug

"PhotonFit has completely removed that friction." - Dr Janice Pata, Wadsworth Centre, NYSDOH. PhotonFit brings smFRET, FCS and FCCS into one intuitive workspace, so researchers can focus on biology instead of debugging code. Watch: www.youtube.com/watch?v=_8S7... #smFRET #FCS

4 Aug

Join Dr Stefan Mueller on 20 Aug to see how FCCS on the EI-FLEX Pro HT directly quantifies ternary complex formation, cooperativity and stability for next-generation therapeutics. Register: us06web.zoom.us/meeting/regi... #ternarycomplex #fccs #proteininteractions #drugdiscovery

buff.lyQuantify ternary complex kinetics with FCCS | Exciting InstrumentsDiscover how FCCS quantifies affinity, cooperativity, and kinetic stability in ternary complexes using dual HER2-binding antibodies.

27 July

Surface immobilisation can limit analysis of transient and multi-component interactions. FCCS measures ternary complex formation, affinity and kinetics directly in solution - no immobilisation or binary kinetics required. See our new application note > buff.ly/jvbB3MY #spr #fccs #ternarycomplex

excitinginstruments.comProtein-driven ssDNA conformational control | Exciting InstrumentsDiscover the linear relationship between FRET efficiency and ssDNA length, and how this can capture protein-mediated conformational control.

23 July

Curious about #smFRET data on #ssDNA? Our latest application note from the Antony Lab (Saint Louis University) shows how EI-FLEX data reveals that RPA wraps ssDNA rather than stretching it, resolving a long-standing question. Read more: buff.ly/N0hSA1h #DNA #AppNote

excitinginstruments.comProtein Ternary Complex Quantification | Exciting InstrumentsSee how FCCS on the EI-FLEX Pro HT quantifies affinity, cooperativity and kinetic stability in HER2–antibody ternary complexes, supporting multispecific drug discovery.

21 July

🙌 New preprint from Exciting Instruments! We show how FCCS directly characterises HER2–trastuzumab–pertuzumab ternary complexes in solution, quantifying affinity, cooperativity & dissociation rates without first establishing binary interactions. Read more: excitinginstruments.com/resource-lib...

20 July

If you work in molecular biology, genomics, biophysics, or biotech, this seminar is worth your time. Prof. Taekjip Ha explores how single-molecule FRET is advancing our understanding of molecular machines, CRISPR, and Prime Editing > 📺 youtu.be/w0Idz5AoW4E?... #singlemolecule #biotechnology

14 July

Headed to the #BBS & #DGfB conference in Glasgow? Meet @romanrenger.bsky.social to discuss single-molecule FRET, FCS, biomolecular dynamics, and complex formation. Catch his talk Wed 3:30pm, visit our booth or poster (Session 2B), or email roman.renger@excitinginstruments.com

13 July

📢 Today's the day! Join Tim Craggs and Prof. Taekjip Ha for this afternoon's Exciting Seminar celebrating 30 years of smFRET. Hear from the pioneer behind the first smFRET measurement and discover what's next. Register: us06web.zoom.us/meeting/regi... #biophysics #singlemoleculeFRET #virtualevent

buff.lyFCS analysis of cell-free protein production | Exciting InstrumentsDiscover a purification-free method using FCS to characterise the real-time cell-free production of chlamydia outer protein B (CopB).

8 July

🔬 Quantify cell-free protein production in real time with FCS - no purification required. Our new application note shows how the EI-FLEX tracked YFP-CopB synthesis over 10 hours and distinguished protein signal from lysate autofluorescence > buff.ly/zp4FsQI

1 July

📢 Join us on 13 July for a virtual seminar on Conformational control: From single-molecule FRET to novel biotechnologies with our CEO Tim and Prof. Taekjip Ha. 11:00am EDT | 8:00am PDT | 4:00pm BST | 5:00pm CEST Register: us06web.zoom.us/meeting/regi... #smFRET #biophysics #singlemolecule

29 June

Struggling to quantify interactions of proteins with binding partners, even in complex environments like the cellular cytoplasm or the cell membrane? Fluorescence correlation spectroscopy (#FCS) could be the answer. Explore practical workflows, applications and FCS/FCCS comparisons: buff.ly/AJl60jr

linkedin.com30 years of single-molecule FRET - a celebration 🎉Hi there, Single-molecule FRET has been transforming how researchers investigate conformational changes, molecular interactions, and complex biological processes for the past 30 years. Today marks 30 ...

25 June

🎉 30 years of single-molecule FRET! Since Taekjip Ha's landmark paper, smFRET has transformed molecular biology. Celebrate with us and share how smFRET has advanced your research: www.linkedin.com/pulse/30-yea... #smFRET

buff.lysmFRET and cryo-EM reveal RNA dynamics | Exciting InstrumentsSee how smFRET on the EI-FLEX, combined with cryo-EM, revealed how dynamic RNA structures regulate RNA-protein interactions and drive flavivirus replication.

24 June

🙌 Congrats to the Bonilla lab on their new preprint! Streit et al. combined cryo-EM, biochemistry, and smFRET on the EI-FLEX to uncover how flaviviral RNA dynamics guide assembly of the replication-initiation complex. A great example of why dynamics matter 💪 buff.ly/r3OHZrY

🎂 As our celebrations of smFRET's 30th birthday continue, here are some key papers from the past three decades. From the first single-molecule study to capturing RNA dynamics, see how smFRET expanded to use alternating laser excitation, 3 colours, and even work in live cells. Papers in the comments.

23 June

3. The first use of alternating laser excitation (ALEX) for smFRET enabled single-molecule analysis of biomolecular structure and interactions simultaneously with smFRET – published by Kapanidis et al., in 2004 - www.pnas.org/doi/full/10....

5. The first use of smFRET in living cells to study conformational changes of SNARE proteins upon complex formation was performed in 2010 by Sakon et al. - www.nature.com/articles/nme...

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4. Also in 2004, Hohng et al. demonstrated the first use of three-colour smFRET using holiday junctions as a model system - extending smFRET's readout beyond a single 1D reaction coordinate - pmc.ncbi.nlm.nih.gov/articles/PMC...

2. Zhuang et al. published the first use of smFRET to track conformational changes of individual ribozymes in 2000, enabling the determination of rate constants and the characterisation of the transition state - pubmed.ncbi.nlm.nih.gov/10856219/

1. The first demonstration that smFRET can track the motion of a single molecule undergoing conformational changes was achieved by Ha et al. in 1999 - buff.ly/neFApLC

18 June

Some molecules don’t like to sit still. That’s why Karim & Roman are heading to #ESAB2026 in #Montpellier! Visit us at booth 5 to explore #smFRET, #FCS & #FCCS, uncover molecular dynamics and interactions in solution, and see how EI-FLEX brings #single-molecule fluorescence into everyday workflows.

16 June

🎉 #smFRET has been transforming how we study molecular interactions and dynamics for 30 years! Join us on 13th July as we celebrate past discoveries, current capabilities, and future frontiers. Featuring Prof. Taekjip Ha, who made the first-ever smFRET measurement. Register: buff.ly/riRgb6b

8 June

👋 Hands up who's at #MachinesonGenes this week! Find Roman to learn how our easy-to-use tools allow you to assess the conformational dynamics and interactions of individual molecular machines. In solution, on the benchtop. Don't miss his talk on Tuesday morning (10:20 am). #smFRET #FCS

excitinginstruments.comEmbracing biophysics in cell lysates or serum | Exciting InstrumentsExplore how the 'messy middle' of biophysics, namely cell lysates and serum, combines single-molecule sensitivity with biological context.

5 June

🐮 What lies between the “spherical cow” of purified proteins and live-cell imaging? The messy middle. Explore single-molecule biophysics in crude lysates and serum, with real-world case studies and practical workflows. excitinginstruments.com/resource-lib... #biophysics #singlemolecule

buff.lyAntibody Fragments | Exciting InstrumentsDiscover EI-FLEX data showcasing how CFPS combined with FCS accelerates the production and characterisation of antibody fragments.

3 June

Antibody screening in hours, not days. LLNL researchers used Fluorescence Correlation Spectroscopy (#FCS) to quantify antibody-antigen binding directly in cell-free lysates with minimal sample consumption - buff.ly/kLhspZX Download our practical FCS handbook here - buff.ly/Dkar7zR #antibodies

29 May

Running smFRET experiments, but unsure how to label your samples or choose the right dyes? Our technical note covers dye selection, site-specific labelling, and key design considerations to help you get reliable data. 📖 excitinginstruments.com/resource-lib... #smFRET #biophysics #biochemistry #howto

26 May

👀 Does the answer to your scientific question depend on understanding how molecules truly interact in solution? 🙌 FCS and FCCS make these measurements possible, at single-molecule sensitivity and without surface immobilisation. 📘 Learn more in our new handbook > buff.ly/CH1uUcm

21 May

🙌 Yesterday at #smbio26 was a shining example of cutting-edge single-molecule science in the UK. The team is on hand again today to discuss how our technology can help you see all the Exciting things your molecules are doing. Want a demo of our system at our nearby HQ? Just let the team know.

20 May

👀 Crystal structures don’t always reveal the full conformational landscape of proteins. Using smFRET + FCS on the EI-FLEX, Howard et al. uncovered two previously unknown salt-dependent conformational states in the bacterial helicase Rep. Read more: buff.ly/smLjyaG #smFRET #FCS #biochemistry

18 May

Who's heading to #smbio26 this week? Catch up with @timcraggs.bsky.social and @romanrenger.bsky.social to discover next-generation single-molecule fluorescence technologies that are helping researchers reveal biomolecular interactions and dynamics with exceptional sensitivity and throughput.

14 May

🤔 Is the ‘average’ molecule a liability in modern drug discovery? Bulk assays hide the transient states that decide whether biologics & TPDs succeed. Discover how smFRET, FCS & FCCS can accelerate drug discovery > buff.ly/fEfiuJM

13 May

🔎 How do antiviral therapeutics work at the molecular level Using smFRET + FCS, Groves et al. investigated how remdesivir and suramin inhibit SARS-CoV-2 RdRp, revealing distinct mechanisms of action beyond gel-based assays. 👀 excitinginstruments.com/resource-lib...

6 May

🇺🇸 Heading to the Industrial Biostructures America #ProterosIBAConference next week? Visit Jake at our booth for an intro to the EI-FLEX Pro, enabling biopharma labs to distinguish individual conformers in solution and quantify biomolecular heterogeneity with ease. #drugdiscovery #biopharma

5 May

🤔 Still wondering how smFRET works in practice - and how it complements your current techniques? Our handbook shows when and why to use it alongside existing methods to add dynamic, single-molecule insight. Download: buff.ly/CxuqL1A #smFRET #biophysics #biochemistry

30 Apr

👋 Run a core facility with cryo-EM or X-ray crystallography? Add smFRET/FCS to reveal dynamics, heterogeneity & interactions in solution - helping your users link structure to function with the EI-FLEX system. excitinginstruments.com/ei-flex/?utm... #biophysics #biochemistry #corefacility

28 Apr

Structures show what #proteins look like - but not how they move. Learn how #smFRET adds dynamics to X-ray, Cryo-EM & AlphaFold to reveal allostery, flexibility & cryptic sites. 👀 buff.ly/c9Bijmx

21 Apr

🔬 Studying #protein-DNAinteractions and wondering how freely-diffusing #single-molecule techniques can support your research? Here are a couple of the most commonly asked questions we hear from scientists in this space. Find more on our protein-DNA application page > buff.ly/uOHzEy5

17 Apr

Find Tim and Charlotte at today's #LondonPolymerGroup meeting (at @imperialcollegeldn.bsky.social) to learn how single-molecule data can complement your existing workflows to reveal polydispersity and #polymer conformational dynamics, and confirm nanoparticle cargo loading or leakage in real-time.

youtu.beProbing Functional Mechanisms of Complex Problems | Prof. Elizabeth Rhoades | Exciting SeminarsYouTube video by Exciting Instruments

14 Apr

👀 How can #FCS be used to quantify interactions of #proteins with binding partners? See how Prof Elizabeth Rhoades' lab used FCS, along with other biophysical methods, as a tool to investigate cellular internalisation of disordered proteins linked to neurodegeneration > youtu.be/Z7nbpW1xBpg?...

10 Apr

🤔 How do #proteins reshape RNA dynamics? Static structures tell only half the story. Using smFRET on the EI-FLEX, the @chillzaa.bsky.social lab at @yorkuniversity.bsky.social mapped viral protein–dependent RNA pseudoknot formation - revealing it as a binary, protein-triggered event > buff.ly/IVQYK72

8 Apr

👀 Your #molecules are doing something exciting! But what, exactly? Discover - when and why - #smFRET is the method of choice to use alongside your current techniques, adding a layer of dynamic data that can quantify #biomolecularconformations and complex formation. Learn more > buff.ly/arAGYZ4

1 Apr

🙌 Congratulations to Chadda et al. on their new paper! #smFRET on the EI-FLEX provided a highly complementary technique alongside SEC, mass spectrometry, mass photometry & bulk FRET to elucidate the pathway that forms mature intasomes from viral #DNA & retrovirus integrase dimers.👀 > buff.ly/mkBUmjc

youtu.beIntroducing the EI-FLEX Pro and Pro HT - for the ultimate single-molecule sensitivityYouTube video by Exciting Instruments

30 Mar

👀 What Exciting things are your molecules really doing? Introducing the EI-FLEX Pro and Pro HT - designed to reveal dynamics, binding, and aggregation in real time with the ultimate single-molecule resolution and no specialist setup > youtu.be/peLNtmqAVIM #singlemolecule #lifescience #biopharma

eventbrite.comDesigning Structure: Protein Therapeutics and VaccinesThe New England Structural Biology Association (NESBA) and NIS are organising a symposium and mixer on April 10, 2026, hosted by Sanofi.

25 Mar

Using #cryoEM or AI to design proteins, but wondering how they behave in solution at the single-molecule level? We’re excited to sponsor NESBA’s Designing Structure symposium. Stop by our booth to chat about uncovering dynamics beyond static structures. 🎟️ www.eventbrite.com/e/designing-...

23 Mar

Download our latest app note (produced in collaboration with the Coleman Lab at Lawrence Livermore National Laboratory) to discover how #FCS can be performed to assess #antibody-antigen binding affinities and generate Kd values for candidate molecules > excitinginstruments.com/resource-lib...