Johannes Hohlbein

So happy to finally see my last work from my time at @mib-wur.bsky.social out! Big thanks to @nicoc-micsynmet.bsky.social, @hohlbeinlab.bsky.social and all other co-authors for their invaluable support throughout the whole process!

How is it going? Problems with prototype autofocus/focus stabilization performance => ask microscopy company to demo their microscope body with (perfect) focus stabilization => focus stabilization unit apparently currently broken awaiting repair... Kafka is calling.

(Cross post LinekdIn) V0.12.1 of #webSMLM released with inbuilt terminal functionality and experimental #smFRET (ALEX-TIRF) implementation.

If you are like me, you start every morning by making yourself a coffee with a moka pot. ☕️ While you wait for the coffee to bubble up, you can now explore how it works: compphys.univie.ac.at/Moka I made this (thank you, Claude!) for my thermo and stat phys course, but perhaps you enjoy it too.

(LinkedIn cross post) 0.12.0 of #webSMLM released. Work mainly by @kjamartens.bsky.social introduced live streaming and real time analysis from frames recorded in MicroManager or Python. hohlbeinlab.github.io/webSMLM/webS...

Update: #webSMLM (V0.11.0), a browser-based single-molecule localisation microscopy analysis tool, includes 2D/3D phasor/MLE fit, background correction, simulations, 3D drift correction, fast temporal median filtering, rendering, spectrally resolved SMLM (!),.. hohlbeinlab.github.io/webSMLM/webS...

📣 We’re hiring a postdoc for @hot-mes-asu.bsky.social ! 🦠🔬 We’re looking for someone excited about microbial interactions, ecology & evolution, and using molecular genetics and microfluidics to understand microbial communities. Come do fun science with us! apply.interfolio.com/190859

Free and open-source images, icons, and tools for creating scientific illustrations doi.org/10.59350/5zt... 🧪

Want to analyse SMLM data on your phone (or any other browser)? Here we go: hohlbeinlab.github.io/webSMLM/webS..., repo on GitHub #webSMLM

Excited to see this collaboration now in print. Dye chemistry meets AI-designed proteins. Three small, orthogonal tags; lifetime modulation; split versions. Request the free dyes at dyes.janelia.org www.science.org/doi/10.1126/...

Nothing fancy, just some old sample doing things earlier today. #FlourescenceFriday

WORK: our Bionanoscience department at TU Delft is looking to hire an Assistant Professor in Biophysics of Living Systems. Apply! Deadline 30 July careers.tudelft.nl/job/Delft-As... RT=nice

You might have heard about our GitHub repository on #OpenMicroscopy. I finally vibecoded a page that (a) allows to search all entries and (b) generates a new database as soon as items are added on GitHub: hohlbeinlab.github.io/OpenMicrosco... Check it out and comments welcome!

Expansion cat. 🐈

I am looking to recruit 2 postdoctoral fellows to the lab to work on a recently funded project focused on class switch recombination in B cells. If you are interested in immunology, gene expression, and RNA and protein single molecule imaging, please reach out (schmi706@msu.edu). Please share!

When chatGPT is drunk..

Join the NWO Physics of Life Seminar on 19 June at Utrecht Science Park Talks by Anna Erzberger (EMBL), Christian Kaiser (UU), Felix Frey (TU/e), Justin Tauber (WUR) Iris Smokers (MPI-CBG) Register <15 June shorturl.at/0VGb7 Organized by @floberger.bsky.social and @uddaloksen.bsky.social

Ordering stuff for the lab is fun. Now I have white board markers but no white board and, even better, two new displays yet the delivery of the PCs got delayed to September 2026... Uff.

🧵 New pre-print out from my time at @mib-wur.bsky.social showing how the titration of the initiator protein DnaA on the chromosome of E. coli can generate stable DNA replication @hohlbeinlab.bsky.social @nicoc-micsynmet.bsky.social doi.org/10.64898/202...

The "Witch of Agnesi" is not what I thought it would be. According to Wikipedia: a cubic plane curve defined from two diametrically opposite points of a circle.

Rough scaffolding of setup #2 done at Brandenburgische Technische Universität Cottbus-Senftenberg. Can actually test here the earlier reported effective vibration isolation for #SMLM using a bicycle inner tube. (Cross post LinkedIn)

Moved some microscopy equipment last week to the new lab. Happy building. (Cross post LinkedIn)

Giving a lecture at Friday 8am is wild. Let’s see.

In academia this weird age is between the age of 25 and 55. At least. Am I right?

Was about to build a new PC for SMLM detection. Memory is ~500 Euros for 32Gb?!?!?! What?

Guess we are living in the future now. Just submitted a manuscript and the website retrieved most information (abstract, names, etc) from the submitted files. I did not have to type everything again! 🤯 (disclaimer: keywords and authors’ contributions were not automatically detected...)

We are very excited to share a new resource from our team: spatial subcellular proteome maps in plants! We developed an MS-based method that registers localizations of about 8000 proteins in Arabidopsis roots in a single experiment. (1/9) www.biorxiv.org/cgi/content/...

New preprint alert! We developed an automated framework to optimize tracking pipelines for bacteria, along with a nice integration of tools for bacteria imaging, in the recently released TrackMate 8 (Fiji plugin). www.biorxiv.org/content/10.6... 👇🧵

iCASE PhD project available in my lab, combining microscopy method development with high throughput screening of bacteria. Applications to antibiotic drug discovery and screening for new cell division proteins. Collaboration with Cairn Research #microsky #bioimaging www.findaphd.com/phds/project...

Great to see our Science Review on emerging multiplexed single-molecule methods that use libraries to link sequence to dynamics. A fun collaborative writing project! From sequence to function: Bridging single-molecule kinetics and molecular diversity | Science www.science.org/doi/10.1126/...

Current mood.

Good resource for everyone interested in objectives and microscopy 👇

@healthcare.nikon.com or others: Where can I find information on the field number of Nikon objectives? Seems missing from the objective comparison site? Specifically wondering about the CFI Apochromat TIRF 60XC Oil.