Jonathan Bohlen

@omuhlemann-lab.bsky.social

🧬 New preprint: "Selection and surveillance of 5S ribosomal RNA genes in human populations." The 5S rRNA genes sit in a ~80-copy array on chromosome 1, one of the least-explored parts of the genome. We traced its variation from DNA to ribosome. 1/4🧵

Jonathan Bohlen reposted

OMühlemann lab

Today, Wojciech Teodorowicz's PhD work on the role of USP9X in occluded A-site RQC was published. Congrats to Wojciech and Lukas, this was not an easy and definitively not a straightforward project. www.cell.com/cell-reports...

cell.comUSP9X promotes the degradation of trapped translation factors on collided ribosomesTeodorowicz et al. show that USP9X, in conjunction with the E3 ligases RNF14 and RNF25, is required to promote the VCP-dependent proteasomal degradation of eRF1 and eEF1A1 trapped in the ribosomal A-site and identify 4EHP as a mediator of translational shutdown following translation factor clearance.

Jonathan Bohlen reposted

LMU München

@lmu.de

🎉 Congratulations to @jonathanbohlen.bsky.social on receiving the Life Sciences Bridge Award from the @avefoundation.bsky.social! The #LMU molecular biologist is being recognized for his research into the molecular mechanisms underlying rare inherited immunodeficiencies. #LMUMunich

lmu.dePrestigious early-career award for LMU molecular biologist Jonathan BohlenLMU molecular biologist Dr. Jonathan Bohlen has received the Life Sciences Bridge Award from the Aventis Foundation.

Jonathan Bohlen reposted

Gene Center Munich @LMU Munich

@genecenter-lmu.bsky.social

Amazing🤩 Congratulations, Jonathan!✨🚀 Prestigious early-career award for LMU molecular biologist Jonathan Bohlen www.lmu.de/en/newsroom/... @jonathanbohlen.bsky.social

lmu.dePrestigious early-career award for LMU molecular biologist Jonathan BohlenLMU molecular biologist Dr. Jonathan Bohlen has received the Life Sciences Bridge Award from the Aventis Foundation.

Vielen Dank an die Aventis Foundation für den Life Sciences Bridge Award 2026! 🏆

Aventis Foundation@avefoundation.bsky.social

This year's “Life Sciences Bridge Award ” goes to Dr. Leonhard Möckl, Dr. Lukas Kunz and Dr. Jonathen Bohlen. Congratulations! Read more: www.aventis-foundation.org and bridge.aventis-foundation.org

Jonathan Bohlen reposted

Evan Karousis

@karousislab.bsky.social

We report a human cell-free translation screen that identified NT-2, a Fusarium mycotoxin that blocks human ribosomes. The surprise: NT-2 does binds the ribosome and pushes them into a dormant state. www.nature.com/articles/s42...

Jonathan Bohlen reposted

rupress.orgHuman-inherited RORγT deficiency encompasses genetic heterogeneity, T cell deficiency, and clinical homogeneityFagniez et al. identify new patients with inherited RORγT deficiency characterized by impaired TCRα rearrangement, depletion of MAIT and iNKT cells, reduce

4 Sept

1/ Delighted to share our paper in @jem.org today, led by Iris Fagniez and @ryangrui.bsky.social, two trainees just starting exciting new chapters in their careers: MD-PhD training at @harvardmed.bsky.social, @mit.edu, and Assistant Prof at @bcmhouston.bsky.social rupress.org/jem/article/...

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We mutated every position of the gene. Variants that block assembly into the ribosome map to the uL18/uL5 interface and activate p53. These are exactly the variants that selection has purged from human populations. 3/4

genesintranslation.comGenes in TranslationAtlases of genetic variation and function across the genes that build the translation machinery.

So the 5S array is a real, functional source of human genetic variation, shaped by the p53 pathway it controls. Explore it in our interactive atlas 👉 genesintranslation.com Preprint 👉 doi.org/10.64898/202... Experimental work by our master's student Laura Sengl, with the whole team. 🙌 4/4

@reeserichardson.bsky.social

Every person carries variant 5S copies, and ~10% of people express variant 5S rRNA. 2/4

Jonathan Bohlen reposted

Reese Richardson

25 Aug

A massive update: At least ~15 companies~ are selling scientists antibodies using faked validation data. We've documented 18,000+ manipulated images on 17,000+ products sold by leading laboratory suppliers including Thermo Fisher, Abcam, Santa Cruz Biotechnology, Millipore Sigma and Bio-Techne. 1/🧵

invt.ioI will present my research at ESID 2026, join meRegister today and save!

7 Aug

Excited to share that I’ll be presenting my work at #ESID2026 in Maastricht! 🇳🇱 You can join the conversation too. Submit your late-breaking research by 30 August & get published in JHI! 🧬 👉 invt.io/1bxbz93skkb #ESID2026 #InbornErrorsOfImmunity #Immunology #IEI

rupress.orgADAR1 loss-of-function variants altering RNA editing define a new interferon-dependent psoriasis subtypeIn this work, we identify a new type I IFN–dependent psoriasis subtype resulting from rare heterozygous variants in ADAR1 and associated with a specific re

12 July

Very interesting paper on defect in RNA editing: rupress.org/jem/article-...

11 July

Happy to see this story out! Congrats to Diana and everyone involved :)

Rockefeller University Press@rupress.org

In @jem.org, Olguín Calderón et al. report four rare or common hypomorphic IL23R variants that, when present in the homozygous state, impair IFN-γ production by innate-like T and NK cells, thereby conferring a predisposition to #tuberculosis. rupress.org/jem/article/... @casanovalab.bsky.social

Jonathan Bohlen reposted

nejm.orgInterleukin-10 Autoantibodies and HLA-DRB1*01:03 in Inflammatory Bowel Disease | NEJMNeutralizing autoantibodies against interleukin-10 can result in a phenocopy of monogenic defects of interleukin-10 signaling in children and may be associated with inflammatory bowel disease (IBD)...

11 June

THIS IS THE BEST PAPER OF 2026 SO FAR, A MONUMENTAL BREAKTHROUGH, A REVOLUTION IN THE FIELD OF INFLAMMATORY CONDITIONS: Interleukin-10 Autoantibodies and HLA-DRB1*01:03 in Inflammatory Bowel Disease | New England Journal of Medicine www.nejm.org/doi/10.1056/...

reeserichardson.blogHow much of Thermo Fisher’s antibody data has been manipulated?We’ve documented more than 100 instances of apparent data manipulation in Thermo’s catalog

29 May

Wow! That's very bad! Great investigative work. @reeserichardson.bsky.social reeserichardson.blog/2026/05/28/h...

11 May

Wow, cool! Adult onset autoimmunity can be genetic.

Journal of Human Immunity@jhumimmunity.org

STING gain-of-function has primarily been described to cause pediatric-onset disease. Here, Riley, Kotzin, Finck, Miner et al. report a surprisingly high prevalence of adult-onset #SAVI among patients with common #autoimmune disease diagnoses. rupress.org/jhi/article/... #Autoimmunity #IEIs

sciencedirect.comRNF25 confers mRNA damage tolerance by curbing activation of the integrated stress responseExcessive RNA damage activates cellular stress responses, triggering cell death. However, pathways that negatively regulate RNA damage responses are l…

24 Mar

Amazing work, contextualizing the mechanism of action of the widely used chemotherapeutic azacytidine. We are very happy to have contributed a little bit to this manuscript! Big congrats to Julian, Carla and everyone involved!

Gene Center Munich @LMU Munich@genecenter-lmu.bsky.social

📣Publication alert: Julian Stingele and his team show that the chemotherapeutic drug azacitidine damages RNA and reveal a possible approach to improve therapies🤩👏 www.sciencedirect.com/science/arti... @jstingele.bsky.social @lmu.de @shubozhao.bsky.social

daimler-benz-stiftung.deDaimler und Benz Stiftung – Stipendiaten 2026Die Daimler und Benz Stiftung gibt Impulse – heute für Morgen. Über Ländergrenzen hinweg fördert sie interdisziplinäre Forschungsprojekte.

10 Mar

I am excited to receive this fellowship, which will help us with our research on regulated mRNA translation in the human immune system! www.daimler-benz-stiftung.de/cms/de/foerd...

4 Mar

I am very excited to share that our paper is on the cover of this month's JHI! rupress.org/jhi/issue/2/2

Jonathan Bohlen reposted

rupress.orgTLR7 mutations leading to enhanced TLR7 signaling in humansThree studies in this issue of JHI extend our understanding of the genetic, molecular, and clinical characteristics of human disease associated with a gain

24 Feb

This is the first News & Views of @jhumimmunity.org and it is a must-read ! TLR7 mutations leading to enhanced TLR7 signaling in humans url: rupress.org/jhi/article/...

Jonathan Bohlen reposted

Journal of Human Immunity

@jhumimmunity.org

6 Feb

Complete and partial forms of X-linked MCTS1 deficiency in patients with mycobacterial disease. New study by Qinhua Zhou (Fudan University), Jacinta Bustamante @institutimagine.bsky.social, Jonathan Bohlen @jonathanbohlen.bsky.social @lmumuenchen.bsky.social & colleagues rupress.org/jhi/article/...

doi.orgComplete and partial forms of X-linked MCTS1 deficiency in patients with mycobacterial diseaseX-linked MCTS1 deficiency causes susceptibility to mycobacterial disease. Four new patients carried three previously undescribed truncating or loss-of-expr

New paper in the Journal of Human Immunity! We report complete and partial forms of X-linked MCTS1 deficiency in patients with mycobacterial disease -- four new families from Iran, Oman, China, and the USA. doi.org/10.70962/jhi...

Building on our 2023 paper, we identified three new MCTS1 variants, including the first hypomorphic variant (p.W175*). Even partial loss of MCTS1 translation reinitiation activity can cause susceptibility to mycobacteria.

Rockefeller University Press

JHI @jhumimmunity.org is the journal of the primary immunodeficiency societies worldwide, hosted by Rockefeller University Press. Open access: doi.org/10.70962/jhi... With Qinhua Zhou, Jacinta Bustamante, Jean-Laurent @casanovalab.bsky.social , and colleagues from six countries.

Jonathan Bohlen reposted

@rupress.org

Journal of Human Immunity

X-linked MCTS1 deficiency causes susceptibility to mycobacterial disease. In @jhumimmunity.org, Zhou et al. show that report 4 new patients carrying 3 previously undescribed truncating or loss-of-expression variants, presenting w/ BCG disease or M. abscessus infection. rupress.org/jhi/article/...

Jonathan Bohlen reposted

@jhumimmunity.org

X-linked MCTS1 deficiency causes susceptibility to mycobacterial disease. Zhou, Bustamante, Bohlen et al. show that report 4 new patients carrying 3 previously undescribed truncating or loss-of-expression variants, presenting w/ BCG disease or M. abscessus infection. rupress.org/jhi/article/...

@biorxiv-cellbio.bsky.social

29 Jan

Our lab website is live! 🥳 We study how mRNA translation controls immune cell function and how defects in the protein synthesis machinery cause human disease. Come say hello and learn about what we do at the Gene Center Munich. bohlenlab.com

Jonathan Bohlen reposted

bioRxiv Cell Biology

27 Jan

Active transport of tRNAs facilitates distributed protein synthesis https://www.biorxiv.org/content/10.64898/2026.01.26.698744v1

Jonathan Bohlen reposted

LMU München

@lmu.de

23 Jan

🙌 Herzlichen Glückwunsch! @jonathanbohlen.bsky.social und Jiamin Hou wurden von der @dfg.de im Rahmen des Emmy Noether-Programms ausgezeichnet. Die Fördersumme beträgt jeweils 1,85 Mio. Euro für einen Zeitraum von sechs Jahren. #LMUMuenchen #Astrophysik #Molekularbiologie www.lmu.de/de/newsroom/...

lmu.deEmmy Noether-Förderung für zwei LMU-ForschendeAstrophysikerin Jiamin Hou und Molekularbiologe Jonathan Bohlen erhalten Förderungen aus dem Emmy Noether-Programm der DFG.

sciencedirect.comCommensal-derived trehalose monocorynomycolate triggers γδ T cell-driven protective ocular barrier immunityCommensals shape host physiology through molecular crosstalk with host receptors. Identifying specific microbial factors that causally influence host …

21 Jan

I don't want to be a huge stickler and I actually really like this paper, congrats to the authors for the nice work. But isn't the use of human eyes in the graphical abstract rather misleading for a paper that contains entirely mouse experiments?

γδ T cell Forum@forum-gd.bsky.social

Commensal-derived trehalose monocorynomycolate triggers γδ T cell-driven protective ocular barrier immunity #gdTcells @cp-immunity.bsky.social

nature.comSomatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and function - Nature ImmunologyBohlen and colleagues report that the E3 ubiquitin ligase CBL is necessary for the development, tolerance and activation of B cells in humans, unlike in mice where CBL deficiency results in loss of T ...

🥳 We are excited to share the first publication from the Bohlen Lab! "Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B but not T cell development and function" www.nature.com/articles/s41... together with the Tangye Lab and @bloodgenes.bsky.social and many more! 1/n

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Huge thanks and congrats go to exceptional first authors Taja Vatovec (in my lab) and my friend Anna-Lena Neehus @alneehus.bsky.social in Boston! Furthermore, many thanks to @casanovalab.bsky.social for letting me start this project in Paris. 5/n

Huge thanks also to all the amazing collaborators and colleagues all around the world, who made this happen. Finally, many, many thanks to the patients and their families for supporting and enabling our research. fin/n

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CBL deficient mice have defects in T cell development and function. Yet, surprisingly, the CBL deficient T cells of the patients were largely unaffected. Conversely, B cell development was strongly perturbed. 3/n

We also found striking defects in B cell function in vivo, by studying the response to SARS.CoV2 mRNA vaccination in a subset of patients. For many more interesting obsevations and experiments, please read the paper! 4/n

We studied patients with somatic CBL deficiency. These patients often have transient myeloproliferation in childhood, and as we published recently (www.jci.org/articles/vie...) chronic autoinflammation. We noticed that our cohort of CBL deficient patients had a high rate of severe infections. 2/n

Gene Center Munich @LMU Munich

16 Dec 2025

Honored to get this opportunity to build out my team and explore molecular immunology!

Gene Center Munich @LMU Munich@genecenter-lmu.bsky.social

🥳🥂Huge congratulations to Jonathan Bohlen on being awarded an Emmy Noether Grant by @dfg.de 😊🤩 His project addresses a central open question in immunology: how mRNA translation governs the function of T cells. www.genzentrum.uni-muenchen.de/news-events/... @jonathanbohlen.bsky.social

Jonathan Bohlen reposted

@genecenter-lmu.bsky.social

25 Nov 2025

📣Publication alert! A team led by Roland Beckmann shows: Ribosome collisions activate the kinase ZAK and thus trigger the ribotoxic stress response — a key mechanism of the cellular stress defense. More about it here👇 www.lmu.de/en/newsroom/...

lmu.deColliding ribosomes signal cellular stressLMU researchers uncover the mechanism by which ribosomes raise alarms in the cell.

Jonathan Bohlen reposted

Beckmann Lab

@beckmannlab.bsky.social

23 Nov 2025

How does the kinase ZAK sense ribosome collisions? Find out in our latest collaboration with the @greenlab.bsky.social @doubleshuang.bsky.social @Vienna Huso: 1/4 rdcu.be/eRmJl #ribosome #cryoEM #LMU #JHMI

Jonathan Bohlen reposted

rupress.orgIRF4 haploinsufficiency in a multiplex family with Whipple’s disease | Journal of Human Immunity | Rockefeller University PressWe report two patients with Whipple’s disease caused by autosomal dominant IRF4 deficiency.

11 Nov 2025

Whipple's disease was an inflammatory condition for decades, it became an infectious disease some years ago, and it is now becoming a genetic disorder: IRF4 haploinsufficiency in a multiplex family with Whipple’s disease url: rupress.org/jhi/article/...

Jonathan Bohlen reposted

rupress.orgJournal of Human Immunity | Rockefeller University PressJournal of Human Immunity (JHI) publishes papers that provide novel insights into the physiology and pathology of human immunity through the study of genetic defects and their phenocopies, including t...

11 Nov 2025

In less than 8 months, @jhumimmunity has published 56 papers (rupress.org/jhi), including 10 already on PubMed (pubmed.ncbi.nlm.nih.gov?term=j+hum+i...). Please submit studies of human inborn errors of immunity and their phenocopies (rupress.org/jhi/article-...) !

link.springer.comA human YEATS4 variant confers resistance to TST and IGRA conversion despite Mycobacterium tuberculosis exposure - Genome MedicineBackground Despite sustained exposure to Mycobacterium tuberculosis (Mtb), some individuals—so-called resisters—have persistently negative results for the tuberculin skin test (TST) and interferon-gam...

20 Oct 2025

We report a 5'UTR variant in YEATS4 that confers resistance to tuberculosis through altering the translation of the YEATS4 open-reading frame! Congrats to Clement Conil and colleagues at @casanovalab.bsky.social! It was fun to help with this story! link.springer.com/article/10.1...

Jonathan Bohlen reposted

Rockefeller University Press

@rupress.org

30 Sept 2025

New in @jhumimmunity.org: Tristan van der Linden, András Spaan et al. describe and characterize six unrelated patients with #OTULIN #haploinsufficiency, in whom severe #necrosis followed infectious and/or traumatic triggers. rupress.org/jhi/article/... #Staphylococcus #Ubiquitin

Jonathan Bohlen reposted

Dorothy Bishop

@deevybee.bsky.social

24 Aug 2025

Huge variability documented in how publishers respond when informed about a problematic body of work by a research group. www.jclinepi.com/article/S089... #publishers #retractions

Jonathan Bohlen reposted

rupress.orgIncomplete penetrance in inborn errors of immunity: A skeleton in the closet—The sequel | Journal of Human Immunity | Rockefeller University PressIncomplete penetrance in genetic disorders can be influenced by genetic variant quality, genetic and epigenetic modification, environment, and mosaicism.

19 Aug 2025

A MUST-READ Review on Penetrance of Human Monogenic Inborn Errors (of Immunity, or not): Incomplete penetrance in inborn errors of immunity: A skeleton in the closet—The sequel url: rupress.org/jhi/article/...

Jonathan Bohlen reposted

Bogunovic Lab

@bogunoviclab.bsky.social

13 Aug 2025

Latest by us! An mRNA-based broad-spectrum antiviral inspired by ISG15 deficiency protects against viral infections in vitro and in vivo | Science Translational Medicine www.science.org/doi/10.1126/...

science.orgAn mRNA-based broad-spectrum antiviral inspired by ISG15 deficiency protects against viral infections in vitro and in vivoA human inborn error of immunity guided discovery and development of a prophylactic, mRNA-based antiviral.

26 July 2025

Excited to participate in this symposium!

Casanova Lab@casanovalab.bsky.social

1/ Join us on 24 Sept 2025 at the @institutimagine.bsky.social in Paris for the Novo Nordisk Prize Symposium: Inborn Errors of Immunity. Registration is free at: my.weezevent.com/novo-nordisk...