Kai Johnsson (@kjohnsson.bsky.social)

Molecular recording of cellular protein kinase activity with chemical labeling: www.nature.com/articles/s41... Congratulations to De-en Sun and the entire team.

nature.comMolecular recording of cellular protein kinase activity with chemical labeling - Nature Chemical BiologyMolecular recorders based on kinase activity-dependent protein labeling track specific kinase activities to understand their link to cellular phenotypes in heterogeneous cell populations and in vivo.

Kai Johnsson

@kjohnsson.bsky.social

9 July 2025

Teaching an old bacterial protein new tricks: Fast, bright and reversible rhodamine tags for live-cell imaging: www.biorxiv.org/content/10.1... Congratulations to Julian Kompa and the entire team.

biorxiv.orgFast, Bright and Reversible Rhodamine Tags for Live-Cell ImagingWe present Rho-tag and SiR-tag, engineered protein tags derived from bacterial multidrug-resistance proteins that bind unsubstituted (silicon-) rhodamines with nanomolar affinity, enabling fast, rever...

Kai Johnsson

@kjohnsson.bsky.social

3 July 2025

Finally out in Nature Chem Bio: SNAP-tag2 for faster and brighter protein labeling www.nature.com/articles/s41... Thank you Steffi and Veselin.

nature.comSNAP-tag2 for faster and brighter protein labeling - Nature Chemical BiologySNAP-tag is a widespread tool for labeling protein for bioimaging. Now, Kühn et al. report SNAP-tag2 with increased labeling kinetics and brightness, which translates into a better performance in live...

Kai Johnsson

@kjohnsson.bsky.social

16 June 2025

Please check out our new high-affinity split-HaloTag for live-cell protein labeling www.biorxiv.org/content/10.1... Congratulations to Yin-Hsi Lin!

biorxiv.orgbiorxiv.org

Kai Johnsson

@kjohnsson.bsky.social

18 Apr 2025

Improving split-HaloTag through computational protein engineering: onlinelibrary.wiley.com/doi/10.1002/...

onlinelibrary.wiley.comImproving split‐HaloTag through computational protein engineeringSplit-HaloTag can be used to transform transient molecular interactions into permanent marks through chemical labeling, thereby enabling the recording of transient physiological events in individual ....

Kai Johnsson

@kjohnsson.bsky.social

7 Feb 2025

Check out our new fluorescent probe for imaging f-actin dynamics: 𝗦𝗶𝗥-𝗫𝗔𝗰𝘁𝗶𝗻: 𝗔 𝗳𝗹𝘂𝗼𝗿𝗲𝘀𝗰𝗲𝗻𝘁 𝗽𝗿𝗼𝗯𝗲 𝗳𝗼𝗿 𝗶𝗺𝗮𝗴𝗶𝗻𝗴 𝗮𝗰𝘁𝗶𝗻 𝗱𝘆𝗻𝗮𝗺𝗶𝗰𝘀 𝗶𝗻 𝗹𝗶𝘃𝗲 𝗰𝗲𝗹𝗹𝘀 www.biorxiv.org/content/10.1... Thank you @veselin-nasufovic.bsky.social

biorxiv.orgSiR-XActin: A fluorescent probe for imaging actin dynamics in live cellsImaging actin-dependent processes in live cells is important for understanding numerous biological processes. However, currently used natural-product based fluorescent probes for actin filaments affec...

You are up to date.