Lopes_Lab

Great science talks are coming up again, via the @genomeiinetwork.bsky.social, in two days from now (Tue Oct 6th). Don't miss them! 👇

keysym.usDNA Replication: Pathway Crosstalk Shaping Physiology and Disease | Keystone SymposiaJoin us at the Keystone Symposia on DNA Replication: Pathway Crosstalk Shaping Physiology and Disease, April 2027, in London, with field leaders!

Working with a great team to organize @keystoneSymposia.bsky.social DNA Replication: Pathway Crosstalk Shaping Physiology and Disease, this April! Join us to explore emerging research in London! keysym.us/KSDNA27 #KSDNA27

nature.comCohesin reshapes replication fork contacts to aid fork slowing and reversal - NatureCohesin-mediated loop extrusion limits sister-fork coupling and tethers nearby replication forks under replication stress, promoting fork reversal and slowing fork progression to safeguard genome...

Thrilled to share a new important study from the lab, published today in @nature.com, showing how cohesin mediates efficient replication fork plasticity and stress response, using its loop extrusion activity to promote 3D contacts on replicating DNA: www.nature.com/articles/s41...

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This was truly a joint effort with Ana Losada, Daniel Giménez-Llorente and Ana Cuadrado at @cniostopcancer.bsky.social, providing essential expertise in 3D genome organization. More help from Melani Rodrigues and Moses Aouami in our lab, and Javier Munoz and Ohri Barroso-Gomila at IIS Biobizkaia.

We are grateful to the @snsf.ch for the generous support to this project, and to @embo.org and Novartis Foundation for their direct support to Daniel’s work in our lab. Thanks also to the Functional Genomic Center Zurich for excellent technical support.

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Mechanistically, our work supports the emerging role of 3D genome organization in genome maintenance and a novel view of the replication stress response that goes beyond local responses to stress and lesions, instead integrating throughout the nucleus via nuclear architecture and organization.

This work largely reflects the creativity, technical skills & impressive resilience of Daniel González-Acosta @daniga90.bsky.social, outstanding postdoc in our lab, currently transitioning towards independence. Dani is a rising star in our field and clearly deserves the largest credit for this work

Combining a new MicroC-based method to map 3D-genome interactions at replicating DNA with genetic tools to inactivate cohesin functions, we show that cohesin accumulates at stressed replication forks and uses loop extrusion to modulate 3D interactions among sister forks and neighboring replicons.

Such fork tethering enables coordinated slowdown of DNA synthesis by preventing PRIMPOL engagement and driving fork reversal. While protecting the genome from replication stress, this may support cancer cell resistance to genotoxic treatments and be targeted or defective in cohesin-mutated tumours.

What if the much-debated mechanisms of PARP inhibitors vary greatly across tissues and tumor subtypes? Our new preprint reveals surprising links between replication fork dynamics and PARPi sensitivity across acute myeloid leukemia (AML) subtypes. www.biorxiv.org/cgi/content/...

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Thus, unlike in solid tumors, PARPi sensitivity in AML appears directly linked to replication fork remodeling, while PRIMPOL-mediated repriming promotes PARPi resistance—likely reflecting exceptionally efficient repair of the resulting ssDNA gaps.

This work was brilliantly led in our lab by Cyril Doerdelmann, assisted by Tristan Gasparetto. Eric CW So's group (Tsz Fung Kan and Larissa Bomfim) made key contributions, and together we are now building on these molecular mechanisms to develop new therapeutic strategies for AML

Strikingly, the exquisite PARPi sensitivity of certain AML lines stems from deregulated RECQ1-mediated restart of reversed forks, causing fork acceleration and breakage. By contrast, MLL-rearranged AMLs escape this cytotoxicity through extensive PRIMPOL-mediated repriming.

PRIMPOL deregulation, both in vitro and in vivo, can drastically alter AML responses to PARPi and standard-of-care treatments, opening promising therapeutic opportunities for treatment-resistant AML.

biorxiv.orgNon-canonical ATR signalling via NBS1 phosphorylation propagates fork slowing from stressed to unperturbed nuclear regionsDNA replication forks frequently encounter obstacles and remodel into four-way junctions to actively slow DNA fork progression. Fork slowing can also spread to undamaged forks via an ATR-dependent mec...

10 July

Thrilled to share a new preprint from the lab, showing that active fork slowing and reversal is not confined to the damaged subnuclear compartment but rapidly extends throughout the nucleus, via non-canonical ATR signaling and modulation of MRN exonuclease activity. www.biorxiv.org/cgi/content/...

10 July

This work was superbly lead by @janakrietsch.bsky.social - senior scientist in our lab - with key contributions from @cejkalab.bsky.social, @smolka-lab.bsky.social, @polosophie.bsky.social and the Sartori lab at IMCR. Curious to hear any feedback on it, while we finalize this work for publication!

8 June

Don't miss the next GiiN seminars tomorrow (June 9th). Just click here at 17.00h CET 👇 uzh.zoom.us/j/6598481621...

5 May

TODAY! Don't miss it 👇

17 Apr

Here is the full 2026/27 program of @genomeiinetwork.bsky.social virtual seminars, chaired by myself and @dimiccolab.bsky.social. Scan the QR code to receive alerts and automated calendar. Don't miss a bunch of exciting talks from established and emerging leaders in genome integrity world-wide!

1 Apr

Another great opportunity for genome integrity / replication stress aficionados to attend two excellent seminars with a simple click. Join us in a few days (Tue April 7th)! 👇

3 Mar

TODAY! 👇

Lopes_Lab@lopeslab.bsky.social

Two more upcoming GIIN virtual seminars you should not miss! Just connect to the Zoom link below on March 3rd, 5pm, CET 👇

26 Feb

Two more upcoming GIIN virtual seminars you should not miss! Just connect to the Zoom link below on March 3rd, 5pm, CET 👇

2 Feb

Tomorrow (Tue 3rd)! PLEASE NOTE THE ALTERNATIVE ZOOM LINK! 👇

uzh.zoom.us/j/6466274666...

2 Feb

uzh.zoom.usJoin our Cloud HD Video MeetingZoom is the leader in modern enterprise cloud communications.

29 Jan

Don't miss the opportunity to attend exciting virtual talks from leading scientists in genome integrity, provided by the @genomeiinetwork.bsky.social! Next one is in a few days 👇

GIIN@genomeiinetwork.bsky.social

Another great science appointment is coming up with @nitikataneja.bsky.social and @doksanilab.bsky.social! Join us live on Tuesday, February 3rd at 5pm (Rome) / 11am (NYC). Zoom link: uniroma1.zoom.us/j/95149984403 Register here to stay in the loop: docs.google.com/forms/d/e/1F...

13 Jan

TODAY! 👇

Lopes_Lab@lopeslab.bsky.social

One more upcoming appointment with great science via the Genome Integrity Italian Network (GiiN). Don't miss it, just join via the link 👇

8 Jan

One more upcoming appointment with great science via the Genome Integrity Italian Network (GiiN). Don't miss it, just join via the link 👇

rdcu.beNucleoplasmic Lamin A/C controls replication fork restart upon stress by modulating local H3K9me3 and ADP-ribosylation levelsNature Communications - Replication fork plasticity upon genotoxic stress is modulated by nuclear architectural components by elusive mechanisms. Here the authors implicate Lamin A/C – best...

19 Dec 2025

Thrilled to share our latest work - now available in full in Nature Communications - led in my lab by @vcherdy.bsky.social, with key collaborative contributions from @nitikataneja.bsky.social . rdcu.be/eVrxX

19 Dec 2025

We link replication fork plasticity to another important player of nuclear architecture, i.e. Lamin A/C, by showing that its nucleoplasmic fraction modulates heterochromatic marks and ADP ribosylation levels at replication factories, thereby controlling RECQ1-dependent restart of reversed forks!

2 Dec 2025

TODAY! Don’t miss it! 👇

Lopes_Lab@lopeslab.bsky.social

In a few days we'll have our last appointment (for this year) with great virtual talks via the GiiN. Please note in agenda and join us with a click! 👇

29 Nov 2025

We will be collecting applications for one more week (Dec 5th), then proceed shortlisting candidates for the next “SNSF starting grant” deadline (May 2026). Please hurry up sending your docs and/or spread the news! (…and mind the “Swiss link” in the eligibility criteria) 👇

Lopes_Lab@lopeslab.bsky.social

New important opportunity for young PI recruitment at our Institute! Please spread the news. Interested candidates should carefully check their eligibility for the indicated SNSF funding schemes, before submitting their application 👍 👇 www.imcr.uzh.ch/dam/jcr:3d1f... www.imcr.uzh.ch/en.html

imcr.uzh.chimcr.uzh.ch

27 Nov 2025

In a few days we'll have our last appointment (for this year) with great virtual talks via the GiiN. Please note in agenda and join us with a click! 👇

30 Oct 2025

Happy to have contributed to this interesting study from penengolab.bsky.social! another interesting example of how chromatin modifications and their readers orchestrate replication fork plasticity and protection 👏👇

The EMBO Journal@embojournal.org

H2B ubiquitination by RNF20/40 safeguards replication fork dynamics in human cells, by facilitating fork reversal and recruiting RNF169 that protects from excessive degradation of nascent DNA @penengolab.bsky.social and collaborators www.embopress.org/doi/full/10....

29 Oct 2025

One more opportunity in a few days (Nov 4th), to attend two great 30' talks online! 👇

11 Sept 2025

imcr.uzh.chimcr.uzh.ch

8 Sept 2025

Tomorrow (Tue 9th) at 5 PM CET. Don't miss it!

Lopes_Lab@lopeslab.bsky.social

Mark your calendar (September 9th) for two more exciting virtual seminars of the Genome Integrity Italian Network (GiiN). Full schedule for the Fall season also provided just below. No registration required, just come and join us with a click! 👇

20 Aug 2025

28 May 2025

One more appointment with great science in a few days, one click away 👇

7 May 2025

Exciting PhD project available in our lab (time sensitive)! 👇

1 May 2025

Exciting science ahead! Hope to see many of you online on Tuesday 6th (just connect, no registration needed)

GIIN@genomeiinetwork.bsky.social

The Genome Integrity Webinar Series continues on Tuesday, May 6th at 5PM (Rome) / 11AM (New York) with an exciting discussion on DNA damage response and repair @kajastelab.bsky.social Join via Zoom: uniroma1.zoom.us/j/95149984403

2 Apr 2025

More exciting genome integrity research at the horizon. Register to receive alerts and marks for your calendar 👍👇

GIIN@genomeiinetwork.bsky.social

We thank @martakova.bsky.social and Mate Maus for sharing their research with our community at yesterday's seminar! Our next seminar on Tuesday, May 6th will feature @kajastelab.bsky.social and Raphael Ceccaldi. Subscribe here to receive the reminder: t.co/Hv6iLmjlly

Exciting seminar series from Genome integrity italian Network (GiiN), chaired by myself and Raffaella Di Micco! Short talks (2x30’), at convenient time for EU/USA attendees. Big shots and raising stars in an expanding field. See flyers + link to receive email alerts. Please repost to your network!👇

Andre Nussenzweig, Michele Pagano, @polosophie.bsky.social Evi Soutoglou, @nitikataneja.bsky.social Marco Tigano, @rubenvanboxtel.bsky.social. Chairs: @lopeslab.bsky.social @dimiccolab.bsky.social Hope to see many of you frequently online!

@alebrambati.bsky.social @cantorlab.bsky.social Raphael Ceccaldi @chowdhurylab.bsky.social @albertociccia.bsky.social Diego Dibitetto, @agagambus.bsky.social @kajastelab.bsky.social @peterlylab.bsky.social @martakova.bsky.social @fmattiroli.bsky.social Mate Maus @centromellone.bsky.social

3 Feb 2025

Just landed back in the lab, after a few incredible days on the Alps! A wonderful opportunity to consolidate our projects, rethink our strategies, explore new ideas, blend as a team... and, above all, have a lot of fun! Proud of you guys... and certainly much stronger now! 👍🤩

19 Jan 2025

a great conference, and a tribute to a great scientist and man 👇

Jihane Basbous@jihanebasbous.bsky.social

Dear Colleagues, We are extending our early bird registration deadline to December 17th as there have been some issues both with the Carte Achat service as well as with the website. All have now been sorted.We look forward to seeing you for this special event that will honour the memory of Angelos.

biorxiv.orgNucleoplasmic Lamin A/C controls replication fork restart upon stress by modulating local H3K9me3 and ADP-ribosylation levelsMild replication interference is a consolidated strategy for cancer chemotherapy. Tolerance to mild replication stress (RS) relies on active fork slowing, mediated by transient fork reversal and RECQ1...

19 Jan 2025

Landing on Bluesky by sharing the latest preprint from the lab: nucleoplasmic Lamin A/C modulates RECQ1-mediated fork restart upon mild genotoxic stress, by regulating heterochromatin marks and ADP-ribosylation levels at replication factories www.biorxiv.org/content/10.1...