Molecular Microbiology

annualreviews.orgCommon Strategies Governing Temperate Phage and α-Herpesvirus PersistenceAll life-forms can be infected by viruses with double-stranded DNA genomes. Typically, permissive cells support a productive infection, where virus gene expression and DNA replication yield virion pro...

Common Strategies Governing Temperate Phage and α-Herpesvirus Persistence: 👇 www.annualreviews.org/content/jour...

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Magnesium induces iron starvation and metabolic rewiring to support the viability of cell envelope mutants and antibiotic-stressed cells: The answer to a long-standing question? pubmed.ncbi.nlm.nih.gov/42922536/ #subtiwiki

biorxiv.orgBacteria hyperpolarize in response to oxygen dropAerobic microbes use oxygen as a terminal electron acceptor to establish an electrochemical gradient across their cell membranes. While this membrane potential (ΔΨ) is essential for multiple cellular ...

Bacteria hyperpolarize in response to oxygen drop: 👇 www.biorxiv.org/content/10.6... #subtiwiki #subtilis

A visit to Göttingen, a city that creates knowledge (Besuch in Göttingen Stadt, die Wissen schafft)

biorxiv.orgDiverse stresses converge on bacterial periplasmic expansion through distinct physiological mechanismsDiverse environmental and antibiotic stresses induce localized periplasmic expansion–– the separation of the inner membrane from the cell wall and outer membrane––in Gram-negative bacteria, yet whethe...

Diverse stresses converge on bacterial periplasmic expansion through distinct physiological mechanisms: 👇 www.biorxiv.org/content/10.6...

nature.comGenetic code expansion enables plant-directed control of bacterial activity - Nature BiotechnologyThe noncanonical amino acid O-methyl-L-tyrosine is used as a tool to control microbial protein synthesis across diverse plants.

Genetic code expansion enables plant-directed control of bacterial activity: 👇 www.nature.com/articles/s41... #subtilis #subtiwiki

pubs.acs.orgPhosphorylation by the PknB Serine/Threonine Kinase Stimulates Dimer Formation by WalRAbstract. WalKR is a two-component system that regulates cell wall homeostasis and other processes in low-GC Gram-positive bacteria. It is essential for vi

👉 Phosphorylation by the PknB Serine/Threonine Kinase Stimulates Dimer Formation by WalR: 👇 pubs.acs.org/bichaw/artic... #subtiwiki

link.springer.comA Bacillus subtilis strain with high proficiency for biofilm formation delays the onset of aging-associated morphological defects in cholinergic neurons of C. elegans - GeroScienceAging is accompanied by progressive functional decline and morphological defects of cholinergic neurons. Some probiotic bacteria with proficiency for biofilm formation in the gut are being discussed a...

👉 Bacillus can do it all: a B. subtilis strain with high proficiency for biofilm formation delays the onset of aging-associated morphological defects in cholinergic neurons of C. elegans! link.springer.com/article/10.1... #subtilis #subtiwiki 🪱🪱🪱

sciencedirect.comQuinone-trapping filaments enhance respiratory capacityCells expand respiratory capacity by adding internal membranes or forming cristae. A new study by Kropp and colleagues shows that the Gram-positive ba…

👉 Quinone-trapping filaments enhance respiratory capacity: www.sciencedirect.com/science/arti... Intrazelluläre Nanotubes, intracellular nanotubes #subtiwiki

a dancing robot in stuggy today: www.regio-tv.de/mediathek/vi... #stuttgart

hobby research 2.0 #mimosine #mimose

An old blossom has produced seeds. I’ve never seen that before...😎

In @eLife.bsky.social: Wow: NUDT2 loss defines quantitative limits for dinucleoside polyphosphate action on the cGAS–STING–TBK1 axis 👇 doi.org/10.7554/eLif... #Ap4A #stress

doi.orgClient Challenge

onlinelibrary.wiley.comTopological, Functional, and Phylogenetic Analysis of the Cell Division Site Selection Protein <fc>MinJ</fc> of <fi>Bacillus subtilis</fi>MinJ is a nine-pass transmembrane protein with a cytoplasmic PDZ domain. The PDZ domain, while required for division, is dispensable for localization, and deletion of the transmembrane segments 6 and...

🫳 Topological, Functional, and Phylogenetic Analysis of the Cell Division Site Selection Protein MinJ of Bacillus subtilis in: Molecular Microbiology | Microbiology Journal | Wiley Online Library onlinelibrary.wiley.com/doi/10.1111/... #subtiwiki #subtilis @molecularmicro.bsky.social

journals.asm.orgRetraction for Hantke et al., Escherichia coli Exports Cyclic AMP via TolC | Journal of BacteriologyVolume 193, no. 5, p. 1086–1089, 2011. We were informed by Kevin Young and Gang Li (Microbiology 160:2079–2088, 2014) that strain H41 is not a tolC mutant as we had described. We have sequenced strain...

journals.asm.org/doi/10.1128/... As a PostDoc, I had to listen to this guy claim that formaldehyde cross-linking doesn't work and that c-di-AMP doesn't exist. Crazy!

Laboratory excursion to the Tübingen Castle Laboratory, where Friedrich Miescher discovered nucleic acid. #tübingen

academic.oup.comDNA polymerase I and DnaE mediate RNA-templated DNA synthesis over embedded ribonucleotidesAbstract. It is established that during normal growth and under stress, ribonucleotides become nested in genomic DNA. Given the high frequency with which R

👉 DNA polymerase I and DnaE mediate RNA-templated DNA synthesis over embedded ribonucleotides: 👇 academic.oup.com/nar/article/... #subtiwiki @narjournal.bsky.social

cell.comA non-canonical redox family protein regulates bacterial interaction machineriesIntercellular communication shapes microbial adaptation to ecological niches and maintains species fitness. Barman et al. reveal that the global regulator YmdB employs the conserved redox-insensitive ...

This really groundbreaking: the MarR family transcription regulator ydzF is recruited by the metallophosphodiesterase YmdB for NANOTUBE production! Do nanotubes really exist? 👇 www.cell.com/cell-reports... #subtiwiki #nanotube #subtilis

nature.comBacterial growth rate in populations and single cells - Nature MicrobiologyIn this Perspective, Levin and Wang give a brief history of bacterial growth rate and discuss how it is connected to nutrient availability and macromolecule synthesis, with a focus on (p)ppGpp as a ce...

super cool perspective article: Bacterial growth rate in populations and single cells! In @natmicrobiol.nature.com 👇 www.nature.com/articles/s41...

biorxiv.orgPhysiological Roles of Carbonic Anhydrases and an MpsAB-like Bicarbonate Transporter in Bacillus subtilisCarbonic anhydrase (E.C. 4.2.1.1) is an enzyme that catalyzes the reversible hydration of CO2 to carbonic acid (H2CO3), which dissociates to bicarbonate (HCO3−) at intracellular pH. Copious amounts of...

🫴 Physiological Roles of Carbonic Anhydrases and an MpsAB-like Bicarbonate Transporter in Bacillus subtilis: 👇 www.biorxiv.org/content/10.6... #subtiwiki #subtilis #CO2

journals.asm.orgThe ytrGABCDEF operon controls cell size, growth arrest, and lysis of distinct Bacillus subtilis subpopulations | Microbiology SpectrumBacillus subtilis is a crucial bacterial model organism with considerable importance to fundamental and applied microbiology, frequently used as a model in bacterial cell biology and physiology and as...

👉 The ytrGABCDEF operon controls cell size, growth arrest, and lysis of distinct Bacillus subtilis subpopulations: | Microbiology Spectrum journals.asm.org/doi/10.1128/...

pnas.orgThe phenotypic landscape of the model firmicute Bacillus subtilis | PNASFirmicutes are gram-positive bacteria with important roles in human health, disease, and industry. However, more than a quarter of genes in the mod...

😎 Read this: The phenotypic landscape of the model firmicute Bacillus subtilis! In @pnas.org : 👇👇 www.pnas.org/doi/10.1073/... #subtiwiki #subtilis

pnas.orgPNASProceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...

Nuclear cGAS inhibits stimulation-induced inflammatory gene transcription via HDAC1: www.pnas.org/doi/10.1073/... #cGAMP @pnas.org

sciencedirect.comsciencedirect.com

Wow: In-cell discovery and characterization of a non-canonical bacterial protein translocation-folding complex! www.sciencedirect.com/science/arti... @cellpress.bsky.social #mycoplasma

journals.asm.orgCraig Venter, a microbiologist | mBioThe death of Craig Venter from a sudden cancer has generated several commentaries and obituaries in important scientific journals to honor the revolutionary innovations he brought to life sciences (1–...

21 Sept

Craig Venter, a microbiologist! In: 👇 | mBio journals.asm.org/doi/10.1128/...

nature.comPyridoxal-phosphate-dependent biosynthesis of aminovaleramide by AvaS in tRNA - Nature Chemical BiologySun, Wu et al. identify AvaS as a pyridoxal-phosphate-dependent RNA-modifying enzyme, producing the tRNA modification ava2C from k2C in bacteria and plants and linking RNA chemistry to translational r...

18 Sept

👉 Pyridoxal-phosphate-dependent biosynthesis of aminovaleramide by AvaS in tRNA: www.nature.com/articles/s41... @natcomms.nature.com

pnas.orgCognitive control in the presence of a gun | PNASGuns are more prevalent in academic spaces than ever before. Some policy makers have proposed that educators carry guns in schools as a means of se...

17 Sept

🤔 Cognitive control in the presence of a gun: 👇 www.pnas.org/doi/10.1073/... @pnas.org

nature.comA glycolytic intermediate gatekeeps global mRNA decay in Escherichia coli - Nature CommunicationsRNA degradation is linked to cellular metabolism, but the underlying mechanisms are not fully understood. Here, the authors show that the glycolytic metabolite fructose 1,6‑bisphosphate inhibits PNPas...

14 Sept

äußerst interessant: the metabolic state of the cell to post-transcriptional control of gene expression! 👇 www.nature.com/articles/s41... @natcomms.nature.com #coli #PNPase

academic.oup.comValidate User

12 Sept

hui: alarmone pGpp inhibits translation through GTP depletion! 👇 academic.oup.com/nar/article/... #subtiwiki #subtilis #alarmone @narjournal.bsky.social

comptes-rendus.academie-sciences.fr The origin of the logic of adaptive mutations: standing by to grow again

10 Sept

😳 Longtemps la réflexion sur l’évolution des espèces a ignoré les microorganismes... comptes-rendus.academie-sciences.fr/biologies/ar... Cet article fait partie du numéro thématique L'évolution biologique coordonné par : Purificación López-García et al.

sciencedirect.comsciencedirect.com

9 Sept

🫴 Long-range mRNA folding shapes expression and sequence of bacterial genes: www.sciencedirect.com/science/arti... #subtiwiki #subtilis

A tabletop plant can serve as inspiration for new experiments: #mimose #mimosin

im Fenster ein Windows Hintergrund #Windows #Microsoft #Gates

9 Sept

the office mimosa is blooming #mimose #mimosa

academic.oup.comAn expanded realm of anti-CRISPR-associated proteins and regulatory mechanismsAbstract. Many bacteriophages encode anti-CRISPR (Acr) proteins that inhibit bacterial CRISPR–Cas immune systems. Rapid acr gene expression upon phage entr

8 Sept

👉 An expanded realm of anti-CRISPR-associated proteins and regulatory mechanisms: academic.oup.com/nar/article/... @narjournal.bsky.social

sciencedirect.comMolecular insights into the promiscuous Ap4N hydrolase YqeKDiadenosine tetraphosphate (Ap4A) and related dinucleoside tetraphosphates (Ap4Ns) are important stress-signalling molecules that coordinate bacterial…

26 Aug

Molecular insights into the promiscuous Ap4N hydrolase YqeK: 👇 www.sciencedirect.com/science/arti... #Ap4A #subtiwiki

journals.asm.org/doi/10.1128/...

8 Sept

journals.asm.orgLoss of the Ap4A hydrolase YqeK impairs stress adaptation and virulence gene expression in Staphylococcus aureus | Journal of BacteriologyStaphylococcus aureus is a leading cause of antibiotic-resistant bacterial infections worldwide and is resistant to many components of the host immune response. Here, we discovered that deletion of Yq...

nature.comColibactin produced by a honeybee gut symbiont mediates pathogen defence - Nature MicrobiologyA colibactin-producing symbiont in honeybee guts defends against opportunistic pathogens and confers protection.

Colibactin produced by a honeybee gut symbiont mediates pathogen defence: www.nature.com/articles/s41... #bee #honey @natmicrobiol.nature.com

academic.oup.comThe Evolutionary Maintenance of Amino Acid Prototrophy in Escherichia coliAbstract. Escherichia coli is a prototroph and can synthesize all twenty proteinogenic amino acids when required to grow in minimal medium. There are appro

super interesting paper: 👇 Evolutionary Maintenance of Amino Acid Prototrophy in Escherichia coli! academic.oup.com/gbe/article/...

I wouldn't fully agree with the paper's main conclusion, because it certainly depends on which enzyme of a biosynthetic pathway is missing.

nature.comThe physicochemical design, structure and permeability of the JCVI-syn3A minimal cell membrane - Nature CommunicationsJCVI-syn3A, a model of minimal cellular life, relies on a cholesterol-rich membrane whose properties remain poorly understood. Here, authors show how cholesterol and sphingomyelin balance membrane ord...

4 Sept

👉 The physicochemical design, structure and permeability of the JCVI-syn3A minimal cell membrane: www.nature.com/articles/s41... #mycoplasma

doi.orgMembrane affinity difference between MinD monomer and dimer is not crucial for MinD gradient formation in Bacillus subtilisDiffusion rate difference between MinD monomer and dimer is important for MinD protein gradient formation in B. subtilis, and not membrane affinity difference.

3 Sept

👉 Membrane affinity difference between MinD monomer and dimer is not crucial for MinD gradient formation in Bacillus subtilis: doi.org/10.7554/eLif... #subtiwiki #subtilis #MinD #MJThiele #formerMScstudent

pnas.orgFlagellar toxicity: Flagellar synthesis is lytic for Bacillus subtilis in the absence of PBP1 | PNASFlagella are large transenvelope nanomachines but how they transit the peptidoglycan in gram-positive bacteria is poorly understood. A recent model...

3 Sept

Wow: Flagellar toxicity: Flagellar synthesis is lytic for Bacillus subtilis in the absence of PBP1! 👇 www.pnas.org/doi/10.1073/... @pnas.org #subtiwiki #subtilis

pnas.orgCold-adapted RNA polymerase from Pseudomonas phage Njord improves synthesis of therapeutic mRNA | PNASAn RNA polymerase identified in the genome of Pseudomonas phage Njord offers a promising tool for the synthesis of mRNA and other therapeutic nucle...

3 Sept

👉 Cold-adapted RNA polymerase from Pseudomonas phage Njord improves synthesis of therapeutic mRNA: www.pnas.org/doi/10.1073/... @pnas.org

www1.wdr.deSie übersprang Schulklassen, forschte in den USA - heute gibt sie Krebspatienten neue HoffnungKinderärztin, Forscherin, Leopoldina-Preisträgerin: Laura Hinze gibt Krebspatienten Hoffnung - und arbeitet 70 Stunden die Woche.

2 Sept

👉 Ihre Forschung gibt Krebspatienten neue Hoffnung: www1.wdr.de/nrw/duesseld... #wdr

journals.asm.orgRedox control and mechanisms of transmembrane signaling in CSS domain c-di-GMP phosphodiesterases that control biofilm formation in Escherichia coli | mBioSensing environmental cues and transmembrane signal transduction via membrane-embedded proteins is a process of key importance in all living cells. To investigate the molecular mechanisms involved, we...

1 Sept

👉 Redox control and mechanisms of transmembrane signaling in CSS domain c-di-GMP phosphodiesterases that control biofilm formation in Escherichia coli: | mBio journals.asm.org/doi/10.1128/...

nature.comQuinone-transporting filaments expand bioenergetic capacity in Gram-positive Bacillota - Nature MicrobiologyBacillus subtilis Ncp and NADH dehydrogenase form tube-like filaments with phospholipid-lined chambers that associate with quinones, which can expand bioenergetic capacity and support respiratory elec...

1 Sept

Quinone-transporting filaments expand bioenergetic capacity in Gram-positive Bacillota: www.nature.com/articles/s41... #subtiwiki

nature.comA c-di-AMP receptor mediates epithelial adhesion of Lactiplantibacillus plantarum - Nature CommunicationsResearchers identified a c-di-AMP receptor that reversibly controls gene expression and host cell adhesion in Lactiplantibacillus plantarum, revealing how this bacterial signal molecule regulates inte...

31 Aug

A c-di-AMP receptor mediates epithelial adhesion of Lactiplantibacillus plantarum: www.nature.com/articles/s41... #cdiAMP @natcomms.nature.com #Lactiplantibacillus