Michael VanInsberghe

Michael VanInsberghe

@mvanins.bsky.social

38 followers54 following12 posts

Michael VanInsberghe reposted

EMBL Events
@events.embl.org

🧬 'Measuring translational dynamics by ribosome profiling' wrapped up yesterday! Huge thanks to the organisers, speakers, and participants who made it a success. Looking forward to continuing the work and seeing you all again soon! 🙌🏼 #EMBORiboProf

Michael VanInsberghe reposted

Jeroen van den Berg
@jervdberg.bsky.social

Happy to share this concise review and outlook for single-cell sequencing methods to unravel cell biology of the nucleus! Big shoutout to my friend and former colleague Peter Zeller for sharing the work! www.sciencedirect.com/science/arti...

sciencedirect.comShining a light on cell biology of the nucleus with single-cell sequencingFrom the preservation of genomic integrity to the regulation of RNA translation, nearly all cellular processes are regulated in a cell context-depende…

Michael VanInsberghe reposted

Marvin Tanenbaum
@marvintanenbaum.bsky.social

Our paper on Stopless-ORF Circular RNAs (socRNAs) is now out in Cell. By high-res tracking and comparing translation by either single or multiple ribosomes, we find that ribosomes cooperate to overcome pausing to ensure fast and efficient translation www.cell.com/cell/fulltex...

cell.comLong-term imaging of individual ribosomes reveals ribosome cooperativity in mRNA translationRibosomes cooperate through transient collisions to ensure efficient translation.

Michael VanInsberghe reposted

Micha Müller
@michamuller.bsky.social

Very happy to share our preprint on visualizing the life cycle of Influenza viruses using single-molecule imaging! 🥳 We developed two techniques to visualize infections of unmodified influenza viruses in live cells from endosomal release to budding of new viruses. For more details&videos see below ⬇️

Michael VanInsberghe
@mvanins.bsky.social

In addition to these methods to profile translation in single cells, there are also several single-cell technologies to measure mRNA features that regulate translation. There wasn’t space to go into detail, but we also included a brief overview of these methods

Michael VanInsberghe
@mvanins.bsky.social

Translation machinery is spatially distributed to modulate expression patterns. RIBOmap from Xiao Wang’s group measures the spatial localization of mRNAs being translated in tissue slices. Signal is only generated when the rRNA-padlock splint, padlock, and RCA primer are colocalized

Michael VanInsberghe
@mvanins.bsky.social

Translation ultimately revolves around an mRNA template. Determining the amounts and states of these templates and how they interact with regulatory machinery is important to understand translational regulation

Michael VanInsberghe
@mvanins.bsky.social

T&T-seq by Kehkooi Kee’s group splits the lysate from a single oocyte, with part used for scRNA-seq, and the other part is used in a RiboLace pull down to select transcripts being actively translated. Highly translated transcripts will be more abundant in the pull down

Michael VanInsberghe
@mvanins.bsky.social

Ribo-STAMP from Gene Yeo’s group instead records ribosome association with RNA edits. Here, a RPS2-APOBEC1 fusion is transiently expressed that is localized to mRNAs by translating ribosomes. Highly translated transcripts will thus accumulate C-U mutations

Michael VanInsberghe
@mvanins.bsky.social

Modifications to this workflow by us (scRibo-seq), the Cenik lab (Ribo-ITP), and Wei Xie (Ribo-lite) increased the sensitivity of traditional ribosome profiling by using different footprint enrichment strategies and efficient library preparation chemistries

Michael VanInsberghe
@mvanins.bsky.social

Ribosome profiling provides a high-resolution snapshot of active translation. By arresting ribosomes on transcripts and digesting away unprotected RNA, you create short ribosome-protected footprints. Sequencing these footprints gives the numbers and locations of ribosomes

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