Nature Protocols

dlvr.itEfficient and precise large-scale genomic duplication using amplification editingNature Protocols, Published online: 26 October 2026; doi:10.1038/s41596-026-01454-7Here, the authors provide a protocol for amplification editing, a genome-editing strategy using prime editors that enables efficient and precise duplication of genomic sequences ranging from tens of base pairs to chromosomal scales in diverse cell types.

New Article! Efficient and precise large-scale genomic duplication using amplification editing

dlvr.itMultiplexed single-cell reduced representation bisulfite sequencing for genome-wide DNA methylation profilingNature Protocols, Published online: 15 October 2026; doi:10.1038/s41596-026-01452-9This protocol outlines multiplexed single-cell reduced-representation bisulfite sequencing (Msc-RRBS), a streamlined and scalable approach for parallel DNA methylation profiling of hundreds of individual cells.

New Article! Multiplexed single-cell reduced representation bisulfite sequencing for genome-wide DNA methylation profiling

nature.comProtocols for asymmetric synthesis and analysis of chiral materialsProtocols for asymmetric synthesis and analysis of chiral materials

Many congratulations to Henri Kagan and Kensō Soai for their work, and for winning the Chemistry Nobel Prize! To celebrate, Nature Protocols has put together a collection of protocols connected with asymmetric synthesis and the analysis of chiral materials. www.nature.com/collections/...

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The 2026 Nobel Prize in Chemistry has been awarded to Henri Kagan and Kensō Soai for "the discovery of non-linear effects and autocatalysis in asymmetric organic synthesis." This Collection celebrates their achievement. #chemsky 🧪

go.nature.comNobel Prize in Chemistry 2026The 2026 Nobel Prize in Chemistry has been awarded to Henri Kagan and Kensō Soai for “the discovery of non-linear effects and autocatalysis in asymmetric ...

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The 2026 Nobel Prize in Physics has been awarded to Francis Halzen "for decisive contributions to the IceCube Neutrino Observatory and the discovery of high-energy neutrinos of astrophysical origin." This collection celebrates the achievement. go.nature.com/4hIf4iN ⚛️🔭🧪

dlvr.itT cell immunomonitoring: a comparative analysis of traditional and novel methods to quantify and characterize human antigen-specific T cellsNature Protocols, Published online: 06 October 2026; doi:10.1038/s41596-026-01453-8We present a comparative analysis review of traditional and cutting-edge technologies for clinical profiling of antigen-specific T cells, highlighting their principles, applications, benefits and limitations and the need for assay harmonization and standardization.

New Article! T cell immunomonitoring: a comparative analysis of traditional and novel methods to quantify and characterize human antigen-specific T cells

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The 2026 Nobel Prize in Physiology or Medicine has been awarded to Karl Deisseroth, Peter Hegemann and Georg Nagel for discoveries leading to optogenetics. This collection celebrates their achievements. #Neuroskyence 🧪

go.nature.comNobel Prize in Physiology or Medicine 2026The 2026 Nobel Prize in Physiology or Medicine has been awarded jointly to Karl Deisseroth, Peter Hegemann and Georg Nagel “for discoveries leading to ...

dlvr.itSIMworks—an integrated software suite for quality-controlled augmented quantitative structured illumination microscopyNature Protocols, Published online: 24 September 2026; doi:10.1038/s41596-026-01444-9SIMworks is an integrated software suite for quality-controlled augmented quantitative structured illumination microscopy (SIM), enabling both new learners and experienced users to implement best practices in SIM imaging.

New Article! SIMworks—an integrated software suite for quality-controlled augmented quantitative structured illumination microscopy

dlvr.itTutorial: interrogating biology using organoid technologiesNature Protocols, Published online: 24 September 2026; doi:10.1038/s41596-026-01447-6This tutorial overviews the use of organoid technologies across a broad spectrum of applications, from investigating cell fate decisions and cell–cell interactions to studying genetic diseases, species evolution and clinical questions.

New Article! Tutorial: interrogating biology using organoid technologies

dlvr.itNew approach methodologies: drug screening platform to identify antifibrotic compoundsNature Protocols, Published online: 24 September 2026; doi:10.1038/s41596-026-01445-8A protocol for screening compounds using human reporter iPS cell-derived cardiac fibroblasts to identify antifibrotic drug candidates with translational potential.

New Article! New approach methodologies: drug screening platform to identify antifibrotic compounds

We have 5 new Collections out! Have a look at our Collections page to see new compilations of protocols on advanced materials, sensing, bioelectronics, metabolomics and proteomics: www.nature.com/nprot/collec...

go.nature.comReframing Precision Medicine: Innovation to Implementation | 13–15 October 2026A Nature conference reimagining what it means to deliver truly personalised care in the next decade.

There is currently a last call for late-breaking abstracts, plus registrations will be closing soon for the Flagship Nature Conference Reframing Precision Medicine: Innovation to Implementation in London from 13–15 Oct. If you wish to join us visit: go.nature.com/471AdQb

dlvr.itTissue expansion mass spectrometry imaging (TEMI) for high-spatial-resolution multiomics molecular mappingNature Protocols, Published online: 21 September 2026; doi:10.1038/s41596-026-01427-wTissue expansion mass spectrometry imaging enables the mapping of lipids, metabolites, proteins, peptides and N-glycans at high spatial resolution.

21 Sept

New Article! Tissue expansion mass spectrometry imaging (TEMI) for high-spatial-resolution multiomics molecular mapping

dlvr.itTrace-n-Seq combines retrograde fluorescent tracing with sorting and single-cell sequencing of innervating peripheral neuronsNature Protocols, Published online: 21 September 2026; doi:10.1038/s41596-026-01416-zThis Protocol uses retrograde axonal tracing and fluorescence-activated cell sorting to transcriptionally profile innervating neurons at single-cell resolution.

21 Sept

New Article! Trace-n-Seq combines retrograde fluorescent tracing with sorting and single-cell sequencing of innervating peripheral neurons

dlvr.itHead-fixed intravenous drug self-administration: a new frontier in addiction neuroscienceNature Protocols, Published online: 17 September 2026; doi:10.1038/s41596-026-01438-7Drug self-administration studies capture the voluntary, goal-directed nature of human drug taking, allowing investigators to dissociate the behavioral and neural mechanisms that motivate drug seeking and taking from the pharmacological effects of drug exposure itself. Now, a protocol provides instructions for hardware assembly and implementation of intravenous drug self-administration in head-fixed mice.

New Article! Head-fixed intravenous drug self-administration: a new frontier in addiction neuroscience

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nature.comMetabolomics and metabolite analysis protocolsThis Collection from Nature Protocols covers metabolomics and metabolite analysis. The protocols cover key stages of metabolite research, including sample ...

Oh we have been busy! Here another collection: @natprot.nature.com The protocols cover key stages of metabolite research, including sample collection, metabolite extraction, chromatographic separation, mass spectrometric analysis and data processing. 🧪 #metabolomics #chemsky

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nature.comProteomics protocolsA collection of proteomics protocols from Nature Protocols focussing on our most recent content.

This collection is on proteomics! @natprot.nature.com A collection of proteomics protocols from Nature Protocols focussing on our most recent content. 🧪 #proteomics #MassSpec

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nature.comProtocols for bioelectronicsA collection of protocols at the interface of electronic, robotic and living systems to fabricate actuators, circuits, robots, wearables and integrated ...

New collection! @natprot.nature.com covering approaches at the interface of electronics, robotics and biological systems. The protocols cover how to design and fabricate robots, actuators and sensors using biological, magnetic and electronic building tools. 🧪 #bioelectronics

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nature.comProtocols for sensingSensing technologies provide us with the ability to detect changes in local chemical, biological or physical environments we would otherwise be unaware of. ...

This collection of protocols covers approaches for sensing using molecular, optical or electrochemical materials adopted for applications in imaging, trace analyte detection and organ-on-chip monitoring. @natprot.nature.com 🧪 #sensors #chemsky #materials

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nature.comProtocols for advanced materialsA collection of articles covering protocols for synthesizing, processing and characterizing advanced materials, with particular emphasis on 2D materials and ...

In this collection we highlight protocols for synthesizing, processing and characterizing advanced materials, with particular emphasis on 2D materials and nanoscale materials. @natprot.nature.com 🧪 #2Dmaterials #materials

lnkd.in#tech #ai #postdocs #students #juniorpis | Thomas Schwarz-RomondScientific publishing - in times of tremendous #tech acceleration and #AI becomes increasingly complex. Giancarlo Porcella Helmholtz PioneerCampus @ Helmholtz Munich recently hosted an editorial round...

A roundtable is an opportunity to gain insight and perspective, we very much enjoy speaking with early career researchers!

Rosy Favicchio@rosyf.bsky.social

Big thank you to @munichimaging.bsky.social and @helmholtzmunich.bsky.social for the hospitality. I had the opportunity to meet with ECRs and PIs to discuss about careers, research publishing and how to incorporate protocols into their strategy.

dlvr.itKit-based remote collection and isolation of human reprogrammable somatic cells for generation of induced pluripotent stem cells and cerebral organoidsNature Protocols, Published online: 16 September 2026; doi:10.1038/s41596-026-01440-zThis protocol reliably generates induced pluripotent stem cells derived from hair follicle keratinocytes.

New Article! Kit-based remote collection and isolation of human reprogrammable somatic cells for generation of induced pluripotent stem cells and cerebral organoids

go.nature.comReframing Precision Medicine: Innovation to Implementation | 13–15 October 2026A Nature conference reimagining what it means to deliver truly personalised care in the next decade.

16 Sept

Last chance to register for Reframing Precision Medicine: Innovation to Implementation, a flagship Nature Conference in London from 13–15 Oct 2026. Learn more at go.nature.com/471AdQb

dlvr.itQuantitative imaging of dynamic processes in intestinal organoidsNature Protocols, Published online: 15 September 2026; doi:10.1038/s41596-026-01432-zWe present a Protocol for long-term imaging of intestinal organoids, in combination with OrganoidTracker software, that enables both single-cell tracking analysis for use in dynamic cell type inference and fluorescence intensity measurements.

15 Sept

New Article! Quantitative imaging of dynamic processes in intestinal organoids

dlvr.itGeneration of eight-cell embryo-like cells from human pluripotent stem cellsNature Protocols, Published online: 11 September 2026; doi:10.1038/s41596-026-01414-1This protocol describes the generation of eight-cell embryo-like cells (8CLCs) from human pluripotent stem cells. The resulting 8CLCs exhibit transcriptional and epigenetic features resembling those of human 8C embryo cells.

11 Sept

New Article! Generation of eight-cell embryo-like cells from human pluripotent stem cells

dlvr.itProbing and controlling Cu catalyst reconstruction during CO2 electroreductionNature Protocols, Published online: 11 September 2026; doi:10.1038/s41596-026-01430-1Copper-based catalysts undergo structural changes during the course of the chemical reaction. This protocol describes how to use in situ/operando analysis using Raman, IR, XPS and XRD to understand Cu reconstruction during CO2 electroreduction.

11 Sept

New Article! Probing and controlling Cu catalyst reconstruction during CO2 electroreduction

dlvr.itnetneurotools: a trainee-oriented approach to network neuroscienceNature Protocols, Published online: 09 September 2026; doi:10.1038/s41596-026-01446-7This is a Perspective covering a Python toolbox for neuroscience research that enables users to fetch datasets, construct networks and null models, estimate graph metrics, fit statistical models and visualize brain images.

9 Sept

New Article! netneurotools: a trainee-oriented approach to network neuroscience

go.nature.comReframing Precision Medicine: Innovation to Implementation | 13–15 October 2026A Nature conference reimagining what it means to deliver truly personalised care in the next decade.

9 Sept

Hannah's looking forward to attending "Reframing Precision Medicine: Innovation to Implementation" in London next month, to learn more about how multi omics, AI & big data can move personalised care from promise to practice. Find out more at: go.nature.com/471AdQb

dlvr.itDopamine polymerization-mediated surface functionalization of living cells for advanced therapeutic applicationsNature Protocols, Published online: 04 September 2026; doi:10.1038/s41596-026-01422-1Here the authors provide a flexible and versatile protocol for surface functionalization of living cells through in situ dopamine polymerization that allows personalized living cells to be designed with customizable functions by tuning the surface components.

4 Sept

New Article! Dopamine polymerization-mediated surface functionalization of living cells for advanced therapeutic applications

dlvr.itVINE-seq and MultiVINE-seq for single-nucleus and multiome profiling of the brain vasculatureNature Protocols, Published online: 03 September 2026; doi:10.1038/s41596-026-01434-xThe cell types forming the blood–brain barrier are underrepresented in existing single-cell studies. This protocol describes the isolation of cerebral vessels and subsequent enzymatic extraction of high-purity nuclei for downstream multiomics.

3 Sept

New Article! VINE-seq and MultiVINE-seq for single-nucleus and multiome profiling of the brain vasculature

dlvr.itSalt-assisted synthesis of high phase-purity metastable 1T′-phase group VIB transition metal dichalcogenidesNature Protocols, Published online: 01 September 2026; doi:10.1038/s41596-026-01429-8The physicochemical properties of transition metal dichalcogenides strongly depend on their crystal phases. This Protocol describes a salt-assisted synthesis of metastable 1T′-transition metal dichalcogenide crystals with high crystalline quality and high phase purity.

1 Sept

New Article! Salt-assisted synthesis of high phase-purity metastable 1T′-phase group VIB transition metal dichalcogenides

dlvr.itShear-flow-induced assembly of 2D nanosheets for the fabrication of composite films with high tensile strengthNature Protocols, Published online: 28 August 2026; doi:10.1038/s41596-026-01442-xWe present a protocol for the fabrication of highly ordered composite films in which 2D nanosheets can be aligned and fixed, yielding high tensile strength and suitability for high-performance wearable electronics.

28 Aug

New Article! Shear-flow-induced assembly of 2D nanosheets for the fabrication of composite films with high tensile strength

dlvr.itPreparation of polycaprolactone-based 2D platelets via living crystallization-driven self-assemblyNature Protocols, Published online: 25 August 2026; doi:10.1038/s41596-026-01428-9Uniform two-dimensional platelets made from synthetic polymers can range in size from 100 nm to several micrometers and have diverse applications. This Protocol describes the synthesis and crystallization-driven self-assembly of poly(ε-caprolactone)-based polymers.

25 Aug

New Article! Preparation of polycaprolactone-based 2D platelets via living crystallization-driven self-assembly

dlvr.itSynthesis of radioafterglow nanoprobes for deep-tissue imaging of hydrogen peroxideNature Protocols, Published online: 24 August 2026; doi:10.1038/s41596-026-01421-2This protocol is for the synthesis of radioafterglow nanoprobes that convert X-ray energy to near-infrared emissions with long half-life and high brightness, detectable from up to 15-cm depth.

24 Aug

New Article! Synthesis of radioafterglow nanoprobes for deep-tissue imaging of hydrogen peroxide

dlvr.itSelective heavy-metal sequestration and rare-earth element recovery using metal–organic frameworksNature Protocols, Published online: 24 August 2026; doi:10.1038/s41596-026-01425-yMetal adsorption using metal–organic frameworks is a good way to remove and recover heavy or rare-earth metals from water in the environment. This protocol describes their design as well as assays to characterize their stability and performance.

24 Aug

New Article! Selective heavy-metal sequestration and rare-earth element recovery using metal–organic frameworks

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20 Aug

An orange-tailed marsh dart damselfly peering through a leaf has won the 2026 BMC Ecology and Evolution and BMC Zoology image competition. The annual contest showcases the beauty of biodiversity and the science behind its conservation: spklr.io/63325ERtjJ #envecon #biodiversity

dlvr.itCentralized processing of frozen tumor tissues for global cancer genomicsNature Protocols, Published online: 17 August 2026; doi:10.1038/s41596-026-01426-xThis Protocol outlines a pathologist-led, centralized workflow for processing frozen tumor tissues to generate high-quality DNA for whole-genome sequencing. It integrates cryosectioning, digital pathology, macrodissection and laser microdissection to enrich tumor content and automated DNA extraction.

17 Aug

New Article! Centralized processing of frozen tumor tissues for global cancer genomics

dlvr.itUsing MetaboAnalyst 6.0 for exposomics data analysis—from LC–MS2 spectra processing to dose–response modeling and causal inferenceNature Protocols, Published online: 17 August 2026; doi:10.1038/s41596-026-01415-0High-resolution liquid chromatography–tandem mass spectrometry is widely used in exposomics, for example, to study metabolomic responses to environmental exposures. This Protocol describes spectra processing, statistical analysis, dose–response modeling and causal analysis using MetaboAnalyst 6.0.

17 Aug

New Article! Using MetaboAnalyst 6.0 for exposomics data analysis—from LC–MS2 spectra processing to dose–response modeling and causal inference

dlvr.itSynthesis of diiron subcluster [Fe2[(μ-SCH2)2NH](CN)2(CO)4]2− for artificial maturation of [FeFe]-hydrogenasesNature Protocols, Published online: 13 August 2026; doi:10.1038/s41596-026-01418-x[FeFe]-Hydrogenases catalyze H2 production and consumption. Their impact is limited by the complexity of the natural synthetic process. This protocol describes the synthesis of diiron subclusters to simplify the production of functional enzymes.

13 Aug

New Article! Synthesis of diiron subcluster [Fe2[(μ-SCH2)2NH](CN)2(CO)4]2− for artificial maturation of [FeFe]-hydrogenases

dlvr.itDirect printing of metasurfaces using formulated optical materialsNature Protocols, Published online: 11 August 2026; doi:10.1038/s41596-026-01407-0A printable composite material provides a practical and cost-effective route for fabricating metasurfaces on flexible or curved substrates, scalable from the UV to IR wavelengths and deployable for photonic devices.

11 Aug

New Article! Direct printing of metasurfaces using formulated optical materials

dlvr.itONE microscopyNature Protocols, Published online: 11 August 2026; doi:10.1038/s41596-026-01399-xAn approach for combining expansion microscopy with fluctuation-based super-resolution analysis enables the visualization of individual proteins via conventional fluorescence microscopy.

11 Aug

New Article! ONE microscopy

dlvr.itStandardized assays for evaluating superoxide dismutase-like and catalase-like activities of nanozymesNature Protocols, Published online: 10 August 2026; doi:10.1038/s41596-026-01417-yAntioxidant nanozymes are therapeutic candidates for oxidative diseases. Here a standardized protocol is described to characterize superoxide dismutase-like or catalase-like nanozymes and quantify their activity.

10 Aug

New Article! Standardized assays for evaluating superoxide dismutase-like and catalase-like activities of nanozymes

dlvr.itDrug self-administration in head-fixed miceNature Protocols, Published online: 10 August 2026; doi:10.1038/s41596-026-01406-1Here, the authors provide a protocol for a head-restrained approach in mice for intravenous and oral self-administration of drug and nondrug rewards.

10 Aug

New Article! Drug self-administration in head-fixed mice

dlvr.itA general approach for the synthesis of nanoisland catalystsNature Protocols, Published online: 05 August 2026; doi:10.1038/s41596-026-01431-0Stabilization of metal single-atom or cluster catalysts is achieved by forming CeOx nanoislands on SiO2 through strong electrostatic adsorption method. Transition-metal precursors are added to form single atoms or clusters on these islands.

5 Aug

New Article! A general approach for the synthesis of nanoisland catalysts

dlvr.itOverestimations in the cyclization efficiency of small, restriction enzyme-digested DNA fragmentsNature Protocols, Published online: 30 July 2026; doi:10.1038/s41596-026-01423-0Overestimations in the cyclization efficiency of small, restriction enzyme-digested DNA fragments

30 July

New Article! Overestimations in the cyclization efficiency of small, restriction enzyme-digested DNA fragments

dlvr.itReply to: Overestimations in the cyclization efficiency of small, restriction enzyme-digested DNA fragmentsNature Protocols, Published online: 30 July 2026; doi:10.1038/s41596-026-01424-zReply to: Overestimations in the cyclization efficiency of small, restriction enzyme-digested DNA fragments

30 July

New Article! Reply to: Overestimations in the cyclization efficiency of small, restriction enzyme-digested DNA fragments

dlvr.itCore2Edge: a human glioblastoma organoid–brain slice model capturing infiltration and transcriptional heterogeneity from core to single-cell dispersionNature Protocols, Published online: 28 July 2026; doi:10.1038/s41596-026-01412-3This protocol describes Core2Edge, a human glioblastoma organoid and brain slice co-culture model that recapitulates tumor infiltration and transcriptional heterogeneity, along with a light-sheet imaging approach to capture the entire model in three dimensions.

28 July

New Article! Core2Edge: a human glioblastoma organoid–brain slice model capturing infiltration and transcriptional heterogeneity from core to single-cell dispersion

dlvr.itThree-dimensional tracking of dynamic structures in living cells using single-plane imaging with focus feedbackNature Protocols, Published online: 28 July 2026; doi:10.1038/s41596-026-01419-wA microscopy approach for the use of focus-feedback for single-plane tracking of subcellular structures during prolonged live-cell fluorescent imaging sessions.

28 July

New Article! Three-dimensional tracking of dynamic structures in living cells using single-plane imaging with focus feedback

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23 July

When I talk to researchers about @natprot.nature.com they are most often surprised by the number of computational protocols we cover. One of the questions I get most often is what are your most popular articles? I linked the readcube links below: 🧪 🧵 #computational #methods

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