Paul Donlin-Asp

Paul Donlin-Asp

@pdaphd.bsky.social

ESAT fellow SIDB University of Edinburgh. opinions solely mine and very ๐Ÿณ๏ธโ€๐ŸŒˆ. https://www.donlin-asp-lab.com/

623 followers1.1K following101 posts

Paul Donlin-Asp reposted

๐Ÿ’ซI have a PhD project with Ryan MacDonald and @pdaphd.bsky.social hoping to find a curious and motivated student. If you are interested, please reach out and follow the instructions to apply. If you are PI with bright students looking for projects, please send them my way! ๐Ÿšจby November 16th ๐Ÿšจ๐Ÿ‘‡๐Ÿผ๐Ÿ‘‡๐Ÿพ๐Ÿ‘‡๐Ÿฟ

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We propose: Synapses donโ€™t lift inhibition by physically removing SynGAP1. They do it by: โ€ข functionally inactivating existing SynGAP1 โ€ข halting its local production โ†’ Translational repression acts as a โ€œplasticity gateโ€ controlling when synapses can strengthen. 6/7

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Despite abundant dendritic Syngap1 mRNA, translation isnโ€™t constant across time. Instead, SynGAP1 synthesis is dynamically repressed: โ€ข during synaptic maturation โ€ข and crucially, transiently suppressed after LTP induction 4/7

We also challenge a key model: SynGAP1 does NOT disperse from synapses during LTP. That effect comes from GFP overexpression artefacts, as the endogenous protein remains stably anchored at the PSD. 5/7

Most studies on locally translated synaptic proteins focus on those that promote strengthening. But what about the brakes? We looked at SynGAP1 โ€” a major negative regulator of Ras/Rap signalling โ€” to ask if it is locally made to constrain plasticity? 2/7

We find SynGAP1 isoforms are tightly developmentally regulated: โ€ข ฮฑ1 = stable synaptic occupancy โ€ข ฮฑ2 = progressively enriched (dominant at synapse) โ€ข ฮฒ = accumulates later in dendrites All isoforms sit right at the PSD. 3/7

Spent 10k on custom media from @thermofishersci.bsky.social which kills our neurons, unlike a similar in house made version. Complained to thermo about this, and after a month of โ€œinvestigationโ€ they refuse to refund or replace it- but will let us have a discount on a replacement. Well done.

Paul Donlin-Asp reposted

Less than a month to go before applications close for the Introduction to Image Analysis workshop in Cardiff (deadline 16th January)! @royalmicrosoc.bsky.social www.rms.org.uk/rms-event-ca...

Crick Image Analysis Team@crick-ia-team.bsky.social

Applications for the next @royalmicrosoc.bsky.social โ€œIntroduction to image analysisโ€ workshop are now open! Join us for 3 days in Cardiff in May to learn the foundations of #bioimageanalysis using Fiji, Python and @napari.org www.rms.org.uk/rms-event-ca...

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On Wednesday I had to say goodbye to my loving George after 13 years. He was sweet, needy, dumb as a rock, and an all around amazing loaf of orange fur. Iโ€™m going to miss this bestest dude.

Paul Donlin-Asp reposted

Iโ€™m thrilled to announce that I will start a postdoc at @pdaphd.bsky.social 's Laboratory at the University of Edinburgh in Scotland ๐Ÿด๓ ง๓ ข๓ ณ๓ ฃ๓ ด๓ ฟ Iโ€™m beyond honored and excited about this opportunity ๐Ÿ™Œ๐Ÿ˜ŠStay tuned for cool microscopy and data! (pic I took back in 2019 secretly wishing to come back)