Philipp Steen

Excellent work, check it out!

allepasse.bsky.social@allepasse.bsky.social

I’m happy to share the main result of my PhD, which you can find on bioRxiv www.biorxiv.org/content/10.6.... If you are interested in learning about a new way to perform DNA-PAINT multiplexing, which we call Combi-PAINT, or if you are interested in the study of mRNA conformation, keep reading! 1/10

How do you scale super-resolution microscopy to dozens of proteins without linearly scaling imaging time? We introduce Combi-PAINT: a combinatorial DNA-PAINT strategy that breaks the 1-target-per-round bottleneck of Exchange-PAINT: www.biorxiv.org/content/10.6... 1/5

biorxiv.orgbiorxiv.org

View full thread

Bonus: We’re not the only ones to come up with an idea like this – @fbzt.bsky.social has developed a closely related technique, so we’re extra confident about applicability and reproducibility! 4/5

Here’s their paper: biorxiv.org/content/10.64898/2026.01.23.701081v1 And our work: biorxiv.org/content/10.64898/2026.01.23.701249v1 Many thanks to @jungmannlab.bsky.social, all the co-authors and everyone who partook in this project! 5/5

biorxiv.orgbiorxiv.org

Core Idea: Targets are not labeled with one unique docking strand. Instead, each target is encoded by a combination of orthogonal docking motifs. Each imaging round reads out one motif → identities are decoded combinatorially. Thus: non-linear scaling of target number with imaging rounds! 2/5

Key achievements: • 41 targets in just 6 rounds. • <30 min total acquisition time for FOVs over 100×100 µm². • ~2.5 nm localization precision. • ~up to 97% decoding accuracy in intact cells. Dive into nanoscale spatial proteomics with standard DNA-PAINT reagents! 3/5