Putrino Lab

Delighted to share Dr Val Rogers’ amazing FREE resource that walks rehabilitation professionals through safe autonomic rehabilitation. A comprehensive resource for anyone treating #LongCOVID, #MECFS and chronic tick/vector borne illness that has an autonomic component. 1/

coresinai.orgManual — The Cohen Center for Recovery from Complex Chronic Illness

We are proud to continue sharing these free educational resources and incredibly grateful to everyone on the team who has contributed to the ATLAS documentation. The ATLAS resources can be accessed below our IACCI manual on this page: coresinai.org/manual Thanks! 🙏 /end

Putrino Lab reposted

Mark Painter

@markmpainter.bsky.social

Replying to @markmpainter.bsky.social

Thanks to collaborations with @virusesimmunity.bsky.social @putrinolab.bsky.social @michaelpelusomd.bsky.social and the help of @polybiorf.bsky.social we were able to perform these analyses on over 500 samples from hundreds of people with LC, recovered controls, and acute SARS-CoV-2 infection.

Putrino Lab reposted

The Friedman Brain Institute

@sinaibrain.bsky.social

A NEW STUDY from @mountsinainyc.bsky.social's David Putrino @putrinolab.bsky.social, Jacqueline Becker, et al. found that people w/ #LongCOVID who used a noninvasive magnetic therapy headset show improved cognitive function & mood. Press Release 🧠 www.mountsinai.org/about/newsro...

Putrino Lab reposted

The Friedman Brain Institute

@sinaibrain.bsky.social

Replying to @sinaibrain.bsky.social

CHECK OUT the Full Study in Brain Communications "Microtesla magnetic therapy for cognitive impairment in long COVID: a randomized pilot study" - Alexandra Canori, David Putrino @putrinolab.bsky.social, Jacqueline Becker, et al. 🧠 academic.oup.com/braincomms/a... @mountsinainyc.bsky.social

@longcovidanswers.bsky.social

2 Sept

Just a reminder (many seem to need it): A failed Paxlovid trial in #LongCOVID does not erase the validity of all the careful work demonstrating SARS-CoV-2 persistence LC, it just shows us that drugs that target acute infection don’t always work on chronic infection. That’s it.

Putrino Lab reposted

Long Covid The Answers

Could Some Long COVID Patients Need Someone Else’s #Mitochondria? Prof @putrinolab.bsky.social discusses different patterns of mitochondrial dysfunction in #LongCOVID &research into whether healthy donor mitochondria could one day be explored for patients with severe dysfunction.

medrxiv.orgMicrotesla Magnetic Therapy for cognitive impairment in post-acute sequelae of SARS CoV-2: A randomized controlled feasibility studyBackground Cognitive impairment has significant implications for function and quality of life and is common in individuals with post-acute sequelae of SARS CoV-2, also known as long COVID (LC). Emergi...

Delighted to announce some new progress on our #LongCOVID trial with Fareon and their first-in-human microtesla magnetic therapy (MMT) device. As you may recall, we pre-printed the safety/feasibility work not too long ago: www.medrxiv.org/content/10.6... In this triple-blind placebo- 1/

test this technology in other diagnoses where we believe neuroinflammation to be driving cognitive symptoms: #concussion, #TBI, #stroke, #mecfs and chronic #lyme. These are incredibly promising results and I'm so grateful to the brilliant team at SomaScan as well as the 9/

globenewswire.comFareon Announces New Paper Linking Biomarkers for Lower Predicted Brain Age, Reduced Inflammation, and Activation of Repair to Its Noninvasive Biophysics TreatmentPlasma proteomics data reveal biological changes in people receiving Fareon Restore™ at home, supporting the potential to treat brain disorders through...

fantastic team from Fareon - these analyses are tricky and the amount of data are overwhelming. Our team is incredibly fortunate to have such great collaborators that are pushing urgently for this technology to be accessible to patients ASAP. Onward! www.globenewswire.com/news-release... /end

Proteins involved in things like increasing autophagy, reducing inflammation, boosting mitochondrial function and these 17 proteins really popped in the analysis that was done by the team, with a 17-protein response score clearly physiologically differentiating MMT responders 7/

from non-responders (NR). We have a long way to go before this is an approved treatment for cog impairment in people with #LongCOVID, but the next phase of this project is to initiate a large-scale, multi-site clinical trial of this technology. In parallel, we are starting to 8/

First finding of note was the responder analysis: encouragingly, in our cohort from the first paper we saw many more responders (R) in the treatment group than the sham group (80% vs. 30%). But then as we dug into the proteomics, we noted that there were 17 different proteins 5/

That really appeared to be associated with clinical response to the MMT therapy (figure below). What was particularly cool about these proteins was that they weren't 'junk' proteins, they were proteins that were legitimately associated with the MMT mechanism of action. 6/

medrxiv.orgPlasma Proteomics Identifies a Microtesla Magnetic Therapy Response Signature in Long COVIDCognitive impairment is a disabling feature of Long COVID with no established disease-modifying therapy, and little is known about the biological changes accompanying clinical improvement. Microtesla ...

control arm. What was cool about this study was that we also captured plasma at different stages of the trial and in collaboration with Somascan #proteomics, we took a look at differences in proteomic readout in the MMT trial participants (preprint👇) www.medrxiv.org/content/10.6... 3/

Somascan uses synthetic DNA probes as molecular detectors, this method can precisely measure over 10,000 proteins simultaneously in blood plasma. This technique is very sensitive to changes in protein expression between timepoints that may be driving improvements in symptoms. 4/

controlled pilot, we showed that MMT was a well-tolerated and feasible intervention that could be deployed remotely in the home. In addition to this, we saw some early signal that it may also be having an effect on patient cognition in the active treatment arm vs. those in the 2/

On the road to Amsterdam to present some of my team's latest research about #LongCOVID at @ISLCPAIS. Very excited to connect with colleagues and share ideas. I've been busy and not on here much, but I see that there has been controversy regarding the use of the phrase 1/

state that although I don't think that PAIS and/or the US variant that has been endorsed by Health and Human Services (HHS) "Infection-Associated Chronic Conditions and Illnesses" (IACCI) are perfect, I think that they serve a purpose and are likely to change in the future. 3/

Ok, so let's get into it: As many of you know, I am the Director of a center that treats #LongCOVID, #MECFS, chronic #Lyme and hypermobility spectrum disorders. At this stage in our learning and evolution, I can say that in the majority (90-95%) of cases, we "treat" and we do 4/

PolyBio Research Foundation

Post-Acute Infection Syndrome (PAIS) and wanted to share my position on the matter. To be clear, this is just my position. Other opinions and positions than mine are valid. No one speaks for everyone on this topic and disagreements can and should happen respectfully. I'll 2/

Putrino Lab reposted

@polybiorf.bsky.social

14 Aug

Featured in Bloomberg this week: UCSF’s PolyBio-supported LIINC team and their recent preprint suggesting SARS-CoV-2 viral and protein persistence in the gastrointestinal tracts of a portion of Long COVID patients. 1/

youtu.beHow to Manage and Treat Post-Exertional Malaise (PEM)YouTube video by RTHM Health

11 Aug

Them: Did you know that people in #longCOVID trials have extremely high placebo rates? Really makes you think…. Me: Oh yeah? What placebo did they use? Them: Nothing crazy, just IV saline Me: 😐😠😡🤬 I wish people understood pathophysiology.

Putrino Lab reposted

Dr Ben King 🌍

@benking01.bsky.social

8 Aug

Replying to @benking01.bsky.social

Good News! The full Webinar has now been uploaded to YouTube. Please share! 'How To Manage And Treat Post Exertional Malaise (PEM) youtu.be/Na5znc92ylY?... #LongCovid #PostExertionalMalaise #PEM #MECFS #PostViralIllness #Webinar @putrinolab.bsky.social

Excited to share some new science in pre-print this morning! We have completed the world's first intravitreal autologous mitochondrial transplant in a human. Let's breakdown what we did and what this means, even starting from the title (!) www.researchsquare.com/article/rs-1... 1/

Finally a huge thank you to our patient and her family. You all are, to me, on the same tier of courage and determination as astronauts exploring the great unknown with us. We will not easily forget the faith and trust that you placed in us to move this research forward for 19/

the millions who can benefit. Feeling enormous gratitude to a whole lot of amazing people who helped us make the impossible possible today. Much more to come! 🙏 /end

from this work - including other forms of brain injury and neurodegeneration, but also #LongCOVID, #MECFS and other complex chronic illness where energy limitation is a problem. We will be aiming to get an active research arm off the ground on this ASAP. Also, hopefully 17/

you can see from this thread that this procedure was successful because a collection of dedicated and clever people worked together across disciplines to achieve something very technically difficult. I cannot fully express the awe and gratitude I have for my co-authors. 18/

saw a really exciting initial response, we also saw that response fall off with >100 days of follow up. Pupillary responses remained higher than they were at baseline, but not as high as we saw them go. So what is next in this exciting work? We are in the process of getting 15/

more procedures lined up for this patient - it is not practical to take a chunk of muscle every time we want to do a transplant, so we're working on some alternate ways to achieve this now and will be resuming the work ASAP. Also, there are so many conditions that can benefit 16/

it like a champ. What was super-encouraging and exciting, though, was that within 48 hours of each transplant we started to see pupillary responses returning to BOTH eyes. We went from pupils that were absent of response to light, to seeing measurable pupillary responses in 13/

both eyes (measured with a device called a 'pupillometer'). We also noted post-transplant that a visual-evoked potential emerged in the brain in response to visual stimuli, meaning that the brain is now responding to visual stimuli that are being presented. Now, although we 14/

injecting mitochondria into the eye would cause an immune reaction. Now, mitochondria themselves are actually famously able to fly under the radar of the immune system and NOT cause an immune reaction, but if the mitochondria get damaged and mitochondrial DNA is released that 11/

is, theoretically, a different story. So our first order of business was to prove that we could perform this transplant without causing a large immune reaction in the eyes and we hit that deliverable handily: no sign of immune reaction in either eye, and our patient handled 12/

from the muscle, isolated the mitochondria and, thanks to our brilliant GMP facility at Mount Sinai led by Mansi Saxena, we put those mitochondria into a syringe for our rockstar Dr Gareth Lema to inject into the right eye of our patient. The next day, we did all over again 9/

for the left eye. Now, as many of you who follow me may know, when we do something first-in-human, yes we are hoping it will work, but first and foremost we're hoping to establish safety. One of the biggest safety concerns we were fielding in this whole process was whether 10/

nature.comTransit and integration of extracellular mitochondria in human heart cells - Scientific ReportsScientific Reports - Transit and integration of extracellular mitochondria in human heart cells

Dr Jim McCully from Harvard, we decided on using muscle tissue and followed his methodology (overseen by Jim himself!) for extracting mitochondria from those cells: www.nature.com/articles/s41... We were also beyond fortunate to have Melanie Walker fly in from Seattle (one of the 7/

few people in the world to have tried mitochondrial transplant of any kind in a stroke patient) to share her wealth of knowledge and experience with us. Procedure day came along: we took a muscle biopsy from the quadriceps (thanks @chriskellnerMD!), extracted mitochondria 8/

nature.comCell-type-targeted mitochondrial transplantation rescues cell degeneration - NatureMitoCatch is a cell-type-specific mitochondrion-targeting system that links mitochondria and the cell surface by protein binders and delivers mitochondria into the target cell.

nerve crush occurs, this is frequently a situation where there are no great options - vision loss is expected to be permanent and there are no known therapies. Our team had been following mitochondrial transplant research and after reading this paper: www.nature.com/articles/s41... 5/

we decided it was worth giving it a shot. We reached out to the FDA and received emergency authorization in record time and began the process. Step 1 was to get tissue from our patient that could be used to extract mitochondria. Working in collaboration with the brilliant 6/

A young person who had survived a severe brain bleed was under our care and recovering from their injury when we noted that they were experiencing vision loss. This happens in some severe circumstances when enough pressure builds up because of the bleed that the optic nerves 3/

(which kind of sit at the bottom of our brains) can get crushed between the base of the skull and the brain itself. In this young stroke survivor, we noted that they were losing pupillary responses day-by-day and their neuroopthalmologist noted total blindness. When optic 4/

nhscotthanekamp.comObituary information for Dr. Brandon Lyon JutrasView Dr. Brandon Lyon Jutras's obituary, contribute to their memorial, see their funeral service details, and more.

Intravitreal: injected into the vitreous humor of the eye Autologous mitochondria: mitochondria that are genetically identical to the transplant recipient and made from their own tissue Transplant: to take from one place and put into another place. So lets dig in: 2/

Putrino Lab reposted

Dr Ben King 🌍

@benking01.bsky.social

6 Aug

Grateful to @putrinolab.bsky.social (and others) for the on-going Webinar, 'How To Manage and Treat Post-Exertional Malaise (#PEM)' YouTube link will follow 🙏 #LongCovid #MECFS

Putrino Lab reposted

Bay Area Lyme Foundation

@bayarealyme.bsky.social

4 Aug

We are heartbroken by the passing of @jutraslab.bsky.social, whom we honored in 2021 with our ELA & whom all of us at BAL considered a friend. His research, mentorship, & partnership leave a lasting impact. His legacy continues to inspire us all. www.nhscotthanekamp.com/obituaries/D...

4 Aug

Anything that publicly earns you praise will always, invariably, earn you hate. This is an immutable law of social media. The trick is to never stop working in good faith to be truly worthy of the praise. That’s what gives you the right to ignore the hate. Thanks for the love 🙏🏻