Herraez Lab

Herraez Lab

@scell-dynamics.bsky.social

Epigenetics & Single Cell Dynamics lab @IBMB_CSIC CRISPR Barcoding | Aging | Stem Cells | Epigenetics | Single-cell multiomics https://herraezlab.rf.gd/?i=1

35 followers84 following5 posts

Herraez Lab
@scell-dynamics.bsky.social

So proud to annouce our new paper in Genome Research ! genome.cshlp.org/content/36/5... Here we present scDynaBar, a CRISPR-based molecular recording system that enables the study of dynamic biological processes at single-cell resolution by accumulating and tracking genomic edits over time ⏰ 🧬 📈

genome.cshlp.orgUsing CRISPR barcoding as a molecular clock to capture dynamic processes at single-cell resolutionBiological processes are inherently dynamic, yet current methods for capturing temporal changes remain limited. Here, we present scDynaBar, a novel approach that combines CRISPR-Cas9 dynamic barcoding with single-cell sequencing. In this system, genetic barcodes gradually accumulate mutations over time; these barcodes are sequenced alongside the transcriptome of individual cells. We propose that the divergence of these barcodes from the original sequence can serve as a record of the timing of cellular events. To demonstrate the potential of this method, we track the transition from a pluripotent state to a two-cell (2C)-like state in mouse embryonic stem cells (mESCs), providing evidence for the transient nature of the 2C-like state. Additionally, our system shows consistent mutation rates across diverse cell types in a mouse gastruloid model, highlighting its applicability to other biological systems. This approach not only improves our ability to study single-cell dynamics but also opens up new possibilities for recording other temporal signals—in other words, using dynamic barcoding as a molecular clock in individual cells.
Genome Research @genomeresearch.bsky.social

The new issue of @genomeresearch.bsky.social is now live. Follow the link to new research on single-cell CRISPR barcoding, Drosophila immune response enhancers, and more! tinyurl.com/Genome-Res-3...

Herraez Lab reposted

CSIC
@csic.es

Replying to @csic.es

🦠Irene Hernando-Herráez, científica del Instituto de Biología Molecular de Barcelona, estudiará las alteraciones de la identidad celular que ocurren con la vejez 🧓"Encontrar la relación entre las diferentes alteraciones acumuladas nos va ayudar a crear estrategias para revertir el envejecimiento"

Herraez Lab reposted

ibmb-csic.bsky.social
@ibmb-csic.bsky.social

Irene Hernando-Herráez, a researcher at the IBMB-CSIC, has been awarded a Starting Grant from the European Research Council (@erc.europa.eu), funded with €1.5 million. These prestigious grants support young scientists leading outstanding research projects. #ERCStG

Herraez Lab
@scell-dynamics.bsky.social

We are so happy to be part of this Network 😊 Thank you for inviting us !

Centro de Biología Molecular Severo Ochoa@cbm-csic-uam.bsky.social

The 2nd Genomic Regulatory Network Annual Meeting, organised by the CBM & funded by @AgEInves, is a great success. Over 3 days, researchers are sharing exciting results, building collaborations, and strengthening connections across institutions. @csic.es @uam.es

Herraez Lab
@scell-dynamics.bsky.social

Welcome to the official bluesky account of the Irene Herraez Lab at @ibmb-csic.bsky.social We study epigenetics & single cell dynamics to understand how cell identity emerges, changes and persists over time 🧫💻👩🏻‍🔬 Excited to share our science with you! 🚀 #Epigenetics #SingleCell #Science

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