Alessandra Brambati

Excited to share our latest from my postdoc at @mskcancercenter.bsky.social in the Sfeir Lab, in collaboration with the Simon Powell Lab! We uncovered a novel genome repair mechanism in human cells: RNA-templated DNA double-strand break repair (RT-DSBR). (1/5)

@genomeiinetwork.bsky.social

22 Jan

Life is difficult but @heyzajr.bsky.social you just made my day better. I love it so much, it’s gorgeous

Alessandra Brambati reposted

GIIN

27 Nov 2025

Join us next Tuesday, December 2 at 5pm (Rome) / 11am (NYC) for an exciting GIIN webinar featuring @peterlylab.bsky.social and @alebrambati.bsky.social! Zoom link: uniroma1.zoom.us/j/95149984403 Register here to become part of our community: docs.google.com/forms/d/e/1F...

Alessandra Brambati reposted

GIIN

@genomeiinetwork.bsky.social

5 Nov 2025

We thank Evi Soutoglou and @rubenvanboxtel.bsky.social for sharing their exciting data with the GIIN community yesterday! Our next webinar is on December 2nd, featuring @peterlylab.bsky.social and @alebrambati.bsky.social. Do not miss it! Sign up for updates: docs.google.com/forms/d/e/1F...

nature.comRNA transcripts serve as a template for double-strand break repair in human cells - Nature CommunicationsDNA double-stranded breaks threaten genome stability. Here, the authors show that transcript RNA serves as a repair template in human cells and identify Polζ as a key factor in RNA-templated DSB repai...

Our study highlights RNA as a direct template for DNA repair, revealing RT-DSBR as a previously unrecognized and potentially mutagenic pathway. Huge thanks to the incredible team who made this work possible! (5/5) 🔗 www.nature.com/articles/s41... #GenomeInstability #DNARepair #RNA #CRISPR #DSB

@hinashah.bsky.social @agnelsfeir.bsky.social

CRISPR/Cas9 screens identified DNA polymerase ζ (Polζ) as essential for RT-DSBR. This translesion polymerase surprisingly acts as a reverse transcriptase—copying RNA into DNA right at break sites. (3/5)

But does RT-DSBR leave a trace? Yes! Analysis of cancer genomes (MSK-IMPACT & PCAWG) revealed whole intron deletions (WIDs)—a mutational signature consistent with RNA being used as a template during repair. (4/5)

Using fluorescent and sequencing-based assays, we show that RNA-containing oligos and even mRNA can serve as templates for DSB repair in human cells. But which enzyme enables this RNA-templated process? (2/5)

Alessandra Brambati reposted

Lopes_Lab

@lopeslab.bsky.social

17 Mar 2025

Exciting seminar series from Genome integrity italian Network (GiiN), chaired by myself and Raffaella Di Micco! Short talks (2x30’), at convenient time for EU/USA attendees. Big shots and raising stars in an expanding field. See flyers + link to receive email alerts. Please repost to your network!👇

Alessandra Brambati reposted

Hoodlum 🇺🇸

@nothoodlum.bsky.social

3 Mar 2025

A brilliant Banksy.

In the midst of all the NIH & NSF chaos, I want to take a moment to focus on some good news and talk about science! 🧬✨ 🚨 New preprint alert! 🚨 doi.org/10.1101/2025... 🧵👇 (1/)

🚀 This study highlights RNA as a direct template for genome repair, revealing RT-DSBR as a previously unrecognized repair pathway with potential mutagenic consequences in cancer. (7/)

Huge thanks to the incredible team who made this work possible! 🙌 Check out the full preprint here: 🔗 doi.org/10.1101/2025... #GenomeInstability #DNARepair #RNA #CRISPR #DSB #Genomics @agnelsfeir.bsky.social @hinashah.bsky.social

In the midst of all the NIH & NSF chaos, I want to take a moment to focus on some good news and talk about science! 🧬✨ 🚨 New preprint alert! 🚨 doi.org/10.1101/2025... 🧵👇 (1/)

🔥 CRISPR/Cas9 genetic screens identified DNA polymerase ζ (Polζ) as the key player in RT-DSBR. This translesion polymerase acts as a reverse transcriptase to copy RNA into DNA at break sites. (5/)

🔬But does RT-DSBR leave a footprint in the genome? YES! We analyzed cancer genome datasets (MSK-IMPACT & PCAWG) and found whole intron deletions (WIDs)—a mutational signature of RT-DSBR. (6/)

In the midst of all the NIH & NSF chaos, I want to take a moment to focus on some good news and talk about science! 🧬✨ 🚨 New preprint alert! 🚨 doi.org/10.1101/2025... 🧵👇 (1/)

💥 DNA double-strand breaks (DSBs) are dangerous lesions that threaten genome integrity. While canonical repair pathways rely on DNA templates, we uncovered an alternative mechanism: RNA-templated DSB repair (RT-DSBR). (3/)

🧬 Using fluorescent and sequencing-based assays, we show that RNA-containing oligos and mRNA can act as templates for DSB repair in human cells. But which enzyme facilitates this process? (4/)

In the midst of all the NIH & NSF chaos, I want to take a moment to focus on some good news and talk about science! 🧬✨ 🚨 New preprint alert! 🚨 doi.org/10.1101/2025... 🧵👇 (1/)

Excited to share our latest work from my postdoc in the Sfeir Lab 🔬, in collaboration with the Simon Powell Lab! 🤝 This project was driven by an incredible team, including the amazing Manisha and superstar Hina! ✨ (2/)

13 Feb 2025

Very excited to be part of the Pharm family! This are confusing time, but I could not ask for a better Department to go through this. Now let’s focus on the science and we will be fine #admaiora

CU Anschutz Department of Pharmacology@cupharmacology.bsky.social

This morning, our department hosted a welcome breakfast for our newest primary faculty member, Assistant Professor Ale Brambati, PhD. We’re thrilled to have her join us, and are so excited for what’s in store for the Brambati Lab! @alebrambati.bsky.social #CUPharmacology #CUAnschutz

2 Dec 2024

Super excited for my first day as Assistant Prof at @cuanschutz.bsky.social in the Dept of Pharmacology! The lab is open, and now I can't wait to do exciting science! #FirstGen #newPI #STEM #AcademicLife #ExcitingScience #PhDLife #WomenInSTEM #MomInSTEM #GenomeStability #CellBiology #DNARepair