Emma R Andersson

I am a huge fan of bioRxiv & medRxiv and Review Commons and I recently spoke with @saramonaco-rc.bsky.social about why. For us, the workflow has been simple and effective:

(6.) Revise, resubmit - and relax a little. Three of our manuscripts have gone through this route, and all quickly found homes: two in EMBO Molecular Medicine and one in Development.

I am a huge fan of bioRxiv & medRxiv and Review Commons and I recently spoke with @saramonaco-rc.bsky.social about why. For us, the workflow has been simple and effective:

(1.) Share the work on bioRxiv as soon as the manuscript is ready. (2.) Get journal-independent, high-quality peer review through Review Commons. (3.) Prepare a clear revision plan in response to the reviews.

🧬 Preprint! 🧵 Our goal is to genetically manipulate every embryonic germ layer to enable high throughput in vivo studies. We previously showed mouse ectoderm can be targeted by amniotic injection, enabling cell-specific manipulation, lineage tracing, and knockouts. But how to reach mesoderm?

Many thanks also to the funders: ERC @erc.europa.eu, Knut and Alice Wallenberg Foundation, Swedish research Council @vetenskapsradet.bsky.social, Leo Foundation, Hjärnfonden, the Göran Gustafsson Prize in Medicine, Karolinska Institutet and the Wellcome Trust.

🧬 Preprint! 🧵 Our goal is to genetically manipulate every embryonic germ layer to enable high throughput in vivo studies. We previously showed mouse ectoderm can be targeted by amniotic injection, enabling cell-specific manipulation, lineage tracing, and knockouts. But how to reach mesoderm?

An enormous effort led by Jingyan He, and Johan Lorentz. Very grateful to excellent lab members and external collaborators also contributing to this project: Agustín Andrés Corbat, Sergey Isaev, Raphaël Mauron, Sandra de Haan @sandehaan.bsky.social, Lenka Belicova @lenkabelicova.bsky.social, (cont)

🧬 Preprint! 🧵 Our goal is to genetically manipulate every embryonic germ layer to enable high throughput in vivo studies. We previously showed mouse ectoderm can be targeted by amniotic injection, enabling cell-specific manipulation, lineage tracing, and knockouts. But how to reach mesoderm?

This is why bypassing Cre drivers matters. Enhancer/promoter-based drivers report marker expression, not ancestry. In organ mesenchyme, transient progenitors share markers, while distinct lineages can converge on similar transcriptional states.

🧬 Preprint! 🧵 Our goal is to genetically manipulate every embryonic germ layer to enable high throughput in vivo studies. We previously showed mouse ectoderm can be targeted by amniotic injection, enabling cell-specific manipulation, lineage tracing, and knockouts. But how to reach mesoderm?

Read the preprint to find out what all the cardiomyocyte and mesenchymal subtypes are, and where they are spatially located 😜

🧬 Preprint! 🧵 Our goal is to genetically manipulate every embryonic germ layer to enable high throughput in vivo studies. We previously showed mouse ectoderm can be targeted by amniotic injection, enabling cell-specific manipulation, lineage tracing, and knockouts. But how to reach mesoderm?

In heart, atrial and ventricular cardiomyocyte lineages are strongly segregated by E7.5. Yet left–right ventricular identities remain embedded within a more plastic continuum of ventricular competence.

🧬 Preprint! 🧵 Our goal is to genetically manipulate every embryonic germ layer to enable high throughput in vivo studies. We previously showed mouse ectoderm can be targeted by amniotic injection, enabling cell-specific manipulation, lineage tracing, and knockouts. But how to reach mesoderm?

(Note: LOTS of hematopoietic cells in the fetal liver, as expected, also lineage traced and with further exciting work to come here!)

🧬 Preprint! 🧵 Our goal is to genetically manipulate every embryonic germ layer to enable high throughput in vivo studies. We previously showed mouse ectoderm can be targeted by amniotic injection, enabling cell-specific manipulation, lineage tracing, and knockouts. But how to reach mesoderm?

So we barcoded mesoderm at E7.5, and analyzed liver development at E16.5 with single cell lineage tracing and VisiumHD to annotate the many new cell types/states in a spatially aware manner, with high confidence.

🧬 Preprint! 🧵 Our goal is to genetically manipulate every embryonic germ layer to enable high throughput in vivo studies. We previously showed mouse ectoderm can be targeted by amniotic injection, enabling cell-specific manipulation, lineage tracing, and knockouts. But how to reach mesoderm?

The scale: >590,000 cells and 31,000 multicellular clones, followed from gastrulation into fetal heart and liver. By combining clonal ancestry, cell state and spatial position, we uncovered developmental histories invisible to transcriptomics alone.

🧬 Preprint! 🧵 Our goal is to genetically manipulate every embryonic germ layer to enable high throughput in vivo studies. We previously showed mouse ectoderm can be targeted by amniotic injection, enabling cell-specific manipulation, lineage tracing, and knockouts. But how to reach mesoderm?

Sometimes a new technology begins with a 60-year-old anatomical drawing. A 1966 image from Biology of the Laboratory Mouse showed a crucial detail: the extraembryonic exocoelomic cavity is continuous with the embryo’s coelomic cavity.

If we lose our inner ear hair cells, we lose our hearing.. but reptiles can repair their sensory organs 🐊🤩 Tune in to Vetenskapspodden on Swedish radio P1, Sun 07:03, Mon 18:33, or bit.ly/4oCDHzP from Sunday, to hear me & Helge Rask-Andersen discuss crocodiles & regeneration with Lena Nordlund!