Daria Van Tyne

Daria Van Tyne

@dariavantyne.bsky.social

Microbiology, evolution, and bacteriophages. University of Pittsburgh School of Medicine.

2.2K followers1K following32 posts

Daria Van Tyne
@dariavantyne.bsky.social

Second, soon-to-defend graduate student @nathaliechen.bsky.social's thesis work on differential virulence in a dominant MDR K. pneumoniae lineage was published in Microbiology Spectrum @asm.org journals.asm.org/doi/10.1128/...

journals.asm.orgDifferential virulence potential of different clades of multidrug-resistant Klebsiella pneumoniae ST258 | Microbiology SpectrumKP ST258 is an epidemic lineage of multidrug-resistant gram-negative bacteria that has caused numerous outbreaks in hospitals around the world. The KP ST258 population is divided into two genetically related but distinct clades, which differ primarily in their capsule type. In this study, we found that patients infected with one of the KP ST258 clades were more acutely ill than patients infected with the other clade. We also observed clade-specific differences both in killing by human serum and in bacterial dissemination in a mouse model of pneumonia. Finally, we identified important limitations in the use of mouse models to study host defenses against multidrug-resistant KP infection. Overall, this work underscores the importance of capsule composition in KP ST258 virulence, identifies differences in the host response to KP infection between mice and humans, and highlights a potential role for complement-targeting immunotherapeutics in the treatment of KP infections.

Daria Van Tyne reposted

Vaughn Cooper
@vscooper.micropopbio.org

Important results of collab led by ⭐surgeon-microbiologist Ken Urish w/ @dariavantyne.bsky.social @ironark.bsky.social and us: Chronic suppression of a multidrug-resistant Pseudomonas aeruginosa in prosthetic joint infection using personalized bacteriophage treatment www.nature.com/articles/s41...

nature.comChronic suppression of a multidrug-resistant Pseudomonas aeruginosa in prosthetic joint infection using personalized bacteriophage treatment - Nature CommunicationsThis work reports the clinical outcomes of bacteriophage therapy for a recalcitrant Pseudomonas aeruginosa periprosthetic joint infection, demonstrating infection control through biofilm disruption an...
Daria Van Tyne
@dariavantyne.bsky.social

We submitted this proposal in January 2025 and it was finally funded! Grateful and excited to keep working to bring phage therapy out of the laboratory and into the clinic. @pghphageprogram.bsky.social www.medschool.pitt.edu/news/pitt-ce...

medschool.pitt.eduPitt Center Is Part of First NIH-Funded Coordinated Research Network for Phage Therapeutics | School of Medicine | University of PittsburghThe National Institute of Allergy and Infectious Diseases (NIAID) has awarded three new Centers for Accelerating Phage Therapy to Combat ESKAPE Pathogens (CAPT-CEP)—the first coordinated U.S. research...

Daria Van Tyne reposted

Pittsburgh Phage Program
@pghphageprogram.bsky.social

Drug-resistant infections are rising—and we need new solutions. Phage therapy uses viruses that target & kill harmful bacteria with precision 🦠 At P3, we’re bringing this innovation to patients who need it most. 🔗 www.futureofpersonalhealth.com/infectious-d... #pittsburghphageprogram #phagetherapy

futureofpersonalhealth.comDrug-Resistant Infections Are Rising. Phage Therapy Can Help Stop Them.Antibiotic-resistant infections are outpacing the drug pipeline, but a powerful, targeted solution has existed in nature all along.

Daria Van Tyne reposted

Tami Lieberman
@contaminatedsci.bsky.social

If you're a senior PhD student or recent graduate interested in doing work at the intersection of AI and microbial genomics and/or microbiomes, please feel free to reach out to discuss the possibility of applying together for an MIT Novo Nordisk Fellowship engineering.mit.edu/novo-nordisk

engineering.mit.eduNovo Nordisk FellowshipPotential areas of focus for postdoctoral fellows participating in the MIT-Novo Nordisk Postdoc Program include, but are not limited to, the following: Your complete application should include: In the...
Daria Van Tyne
@dariavantyne.bsky.social

Two new studies from us using culture-enriched metagenomics to study populations (not just clones!) of some of our favorite superbugs collected from infected patients in the hospital 🧵

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Daria Van Tyne
@dariavantyne.bsky.social

We again used culture-enriched metagenomics to study VREfm populations from the GI tract and bloodstream of 35 patients. Not surprisingly (but still good to confirm!), GI tract populations had greater diversity and population sequencing uncovered more putative transmission than WGS of clones.

Daria Van Tyne
@dariavantyne.bsky.social

Two new studies from us using culture-enriched metagenomics to study populations (not just clones!) of some of our favorite superbugs collected from infected patients in the hospital 🧵

View full thread
Daria Van Tyne
@dariavantyne.bsky.social

We profiled P. aeruginosa populations from six patients over time as they evolved increasing AMR. Population sequencing identified resistance-associated mutations at low frequency in some populations; many of these mutations were not found in single-colony isolates from the same sample.

Daria Van Tyne
@dariavantyne.bsky.social

Study 2: Culture-enriched metagenomic sequencing reveals within-patient diversity and transmission of vancomycin-resistant Enterococcus faecium www.medrxiv.org/content/10.6...

medrxiv.orgCulture-enriched metagenomic sequencing reveals within-patient diversity and transmission of vancomycin-resistant Enterococcus faeciumColonization of the gastrointestinal (GI) tract by vancomycin-resistant Enterococcus faecium (VREfm) often precedes bloodstream infection and serves as a reservoir for onward patient transmission in h...
Daria Van Tyne
@dariavantyne.bsky.social

Two new studies from us using culture-enriched metagenomics to study populations (not just clones!) of some of our favorite superbugs collected from infected patients in the hospital 🧵

Daria Van Tyne
@dariavantyne.bsky.social

Study #1: Within-patient evolution of Pseudomonas aeruginosa populations during antimicrobial treatment journals.asm.org/doi/10.1128/...

journals.asm.orgWithin-patient evolution of Pseudomonas aeruginosa populations during antimicrobial treatment | mSpherePseudomonas aeruginosa infections are notoriously difficult to treat and are associated with high rates of morbidity and mortality. While the genetic basis of resistance in P. aeruginosa is well docum...
Daria Van Tyne
@dariavantyne.bsky.social

This was so much fun! Check out Jess’s thread and the podcast to hear how we “do” phage therapy these days @pghphageprogram.bsky.social

Jessica Sacher@jessicasacher.bsky.social

In 2018, @dariavantyne.bsky.social started her phage lab at @pittdeptofmed.bsky.social. She had 0 phages, just knew their biology & wanted to help patients. Today her lab makes phage for dozens of patients/year under compassionate use, while still publishing research. We asked Daria: HOW? 🧵1/

Daria Van Tyne
@dariavantyne.bsky.social

Submission deadline for short talks and scholarships is Wednesday! Come join us in Breckenridge in May!

Keystone Symposia@keystonesymposia.bsky.social

⏰ Deadline Jan 7 (11:59 pm MST) to submit a short talk abstract or apply for a scholarship for the Keystone Symposium Beyond Antibiotics. Hear from @joeyzacks.bsky.social, @maribyndloss.bsky.social: youtu.be/8Ppjx-MnvxA keysym.us/KSBeyondAnti... @kimingeneva.bsky.social & @dariavantyne.bsky.social

youtu.beKSQA: Drs. Joseph P. Zackular & Mariana X. Byndloss (Beyond Antibiotics)YouTube video by KeystoneSymposia
Daria Van Tyne
@dariavantyne.bsky.social

So incredibly proud of this work, and thankful to everyone who makes it possible!

Pittsburgh Phage Program@pghphageprogram.bsky.social

Pittsburgh Phage Program — 2025 Wrapped! To our patients, clinicians, researches, regulators, and partners— this work doesn’t happen without you. We screened, treated, delivered, learned—and even laid the groundwork for a 2026 trial! #pittsburghphageprogram #phagetherapy #P3

Daria Van Tyne reposted

Kimberly Kline 🏔
@kimingeneva.bsky.social

We're just 6 months away from @keystoneSymposia.bsky.social Beyond #Antibiotics : Emerging Strategies Combating #BacterialInfection, May 2026 in Breckenridge! 👉Scholarship and short talk abstract deadlines are Jan 7! 🧐 keysym.us/KSBeyondAntibiotics26 #KSBeyondAntibiotics26

keysym.usBeyond Antibiotics: Emerging Strategies for Combating Bacterial Infection | Keystone SymposiaJoin us at the Keystone Symposia on Beyond Antibiotics: Emerging Strategies for Combating Bacterial Infection, May 2026, in Breckenridge, with field leaders!
Daria Van Tyne
@dariavantyne.bsky.social

Come join us in Breckenridge next May!

Joe Zackular@joeyzacks.bsky.social

Delighted to announce the @keystonesymposia.bsky.social meeting: Beyond #Antibiotics: Emerging Strategies Combating #BacterialInfection. Join us in Breckenridge in May 2026!! You can check out the program and amazing lineup of speakers below: keysym.us/KSAntibiotics26 #KSAntibiotics26

keysym.usBeyond Antibiotics: Emerging Strategies for Combating Bacterial Infection | Keystone SymposiaJoin us at the Keystone Symposia on Beyond Antibiotics: Emerging Strategies for Combating Bacterial Infection, May 2026, in Breckenridge, with field leaders!

Daria Van Tyne reposted

Jesse Shapiro
@bjesseshapiro.bsky.social

Come join us at @mcgill.ca as an assistant professor in virus-host interactions! Please share widely. mcgill.wd3.myworkdayjobs.com/en-US/McGill...

mcgill.wd3.myworkdayjobs.comAssistant Professor (Research), Department of Microbiology & Immunology, (C2-240724)Please refer to the How to Apply for a Job (for External Candidates) job aid for instructions on how to apply. If you are an active McGill employee (ie: currently in an active contract or position at ...

Daria Van Tyne reposted

Pittsburgh Phage Program
@pghphageprogram.bsky.social

Big shoutout to @olayarendueles.bsky.social @julielebris.bsky.social , @nathaliechen.bsky.social, @dariavantyne.bsky.social and Adeline Supandy—such an awesome team effort from the #VanTyneLab at Pitt! 💫 Great to see phage work move forward together! #PittsburghPhageProgram

Olaya Rendueles@olayarendueles.bsky.social

Our review on recent advances in #PhageTherapy against Klebsiella pneumoniae is out! We analyze KP-phage interactions from an eco-evo perspective, summarize many lab and clinical trials, and discuss novel approaches like genetic engineering & machine learning. #microsky doi.org/10.1371/jour...

doi.orgPhage therapy for Klebsiella pneumoniae: Understanding bacteria–phage interactions for therapeutic innovationsKlebsiella pneumoniae (KP) is a Gram-negative bacterium that commonly resides in the human gastrointestinal tract and can also act as an opportunistic pathogen and cause extra-intestinal infections. K...

Daria Van Tyne reposted

Pittsburgh Phage Program
@pghphageprogram.bsky.social

Behind every patient story, every discovery, and every #phage, there’s a dedicated team making it happen. Over the next few weeks, we’re excited to introduce the people who power the #PittsburghPhageProgram — starting with Dr. Ghady Haidar.🧫✨

Daria Van Tyne
@dariavantyne.bsky.social

Finally, we wondered whether the strain replacement we observed at our hospital was a widespread phenomenon by examining over 15,000 publicly available VREfm genomes. We found that what is happening at UPMC is also happening all over the world. Global genomic surveillance for the win!

Daria Van Tyne
@dariavantyne.bsky.social

We observed large-scale strain replacement just in the 6 years of our study, and Emma figured out that this was likely due to production of a bacteriocin called T8, which is carried on a plasmid, kills enterococci, and was previously studied as a probiotic for the treatment of intestinal dysbiosis.

Daria Van Tyne reposted

Pittsburgh Phage Program
@pghphageprogram.bsky.social

👋 We're the Pittsburgh Phage Program (P3)! Based at Pitt, we advance precision medicine using phages—viruses that kill bacteria. We fight antibiotic resistance through compassionate use & clinical trials. Follow for 🔬research, 💡phage facts, 📢success stories! #PhageTherapy #P3

Daria Van Tyne reposted

Vaughn Cooper
@vscooper.micropopbio.org

How does avi-caz treatment lead to broad-spectrum treatment failure? Our awesome Peds ID faculty and labbie @rapskiid.bsky.social shows how, by longitudinal sequencing, genotyping, and phenotyping. Congrats Glenn! @dariavantyne.bsky.social @aleciarokes.bsky.social journals.asm.org/doi/10.1128/...

journals.asm.orgMutations leading to ceftolozane/tazobactam and imipenem/cilastatin/relebactam resistance during in vivo exposure to ceftazidime/avibactam in Pseudomonas aeruginosa | Microbiology SpectrumAntibiotic resistance is a significant challenge for physicians trying to treat infections. The development of novel antibiotics to treat resistant infections has not been prioritized for decades, lim...

Daria Van Tyne reposted

Willem van Schaik
@wvschaik.bsky.social

JOB CLAXON! I am recruiting a post-doctoral researcher on a BBSRC-funded project (36 months) on the genetics and functional genomics of vancomycin resistance in enterococci. Further details here: edzz.fa.em3.oraclecloud.com/hcmUI/Candid... Please DM or email me if you want to discuss this post.

edzz.fa.em3.oraclecloud.comResearch Fellow - Department of Microbes, Infections and Microbiomes - 104864 - Grade 7This exciting BBSRC-funded project aims to mechanistically characterise the fitness costs associated with vancomycin resistance in the Gram-positive opportunistic pathogens Enterococcus faecalis and E...

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