Jan Philipp Kreysing

Delighted that our story on chromatin architecture inside human cells is now published — the last chapter of my PhD. It was a pleasure working with @sergiocruzleon.bsky.social, @johannesbetz.bsky.social, and everyone involved. #teamtomo www.nature.com/articles/s41...

I'm very grateful to the SCALE Cluster of Excellence for this honor, and to my supervisor, @becklab.bsky.social, for his fantastic mentorship throughout my PhD.

Systematic membrane thickness variation across cellular #organelles revealed by #cryoET. New computational workflow developed by @deglushk.bsky.social, Stefanie Böhm, and @becklab.bsky.social: rupress.org/jcb/article/... 📕 Part of our #Tools collection: rupress.org/jcb/collecti...

Our software webinar series will resume in October with Beata Turoňová, Group Leader at the @mpibp.bsky.social, introducing cryoCAT. Until then, you can catch up on webinars you missed from our last series on the SBGrid YouTube channel. More here: buff.ly/p1w3J7S #SBGrid #Webinars

PhD: defended ✅🎉 My work used in situ cryo-ET #teamtomo to investigate cellular machines from ribosome translation and HIV capsid at the nuclear pore to membrane association of vault, and the molecular architecture of heterochromatin. Huge thanks to @becklab.bsky.social. What a wonderful ride!

We have published a review on how contextual information can complement and enhance research using #cryoET . www.sciencedirect.com/science/arti...

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

Out now in Nature Communications! We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky www.nature.com/articles/s41...

Updated architectural model of the NPC from our lab, starred by bsky-less Agnieszka Obarska-Kosińska. Using cryo ET, XL-MS and modeling, we identify novel nucleoporins, among those the mRNA export complex TREX-2! Check out the preprint on biorxiv #TeamMassSpec #TeamTomo

Ready to TANGO? 💃 In our new publication, Beata Turoňová & Markus Schreiber (@ms314.bsky.social, @becklab.bsky.social) present TANGO: the first cryoET framework to analyze the relation between particle positions and orientations 🪩 All in a single user-friendly package! #CryoET #OpenScience

We are excited to share our new preprint which is now available to read on biorXiv: doi.org/10.64898/202... 🎉🎉🎉

#teamtomo strikes again! Check out our new preprint on the enigmatic vault and its newly discovered association with membranes in situ.

New paper out! How can we force HIV genomes out of their capsid shells and what can we learn about HIV biology doing so? Hint: capsid inhibitors actively re-route nuclear HIV complexes, and expose genomes via intriguing structural effects! open access: link.springer.com/article/10.1... read below

Exciting new paper from my colleague @deglushk.bsky.social in the @becklab.bsky.social! It introduces a fast, robust tool for measuring membrane thickness in tomograms #TeamTomo.

Have you registered to the next EMBO Practical Course on In-situ structural biology by Cryo-FIB and Cryo-ET? 🧊 Join us in Frankfurt for hands-on training on cryo-techniques and networking with experts! 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-... 📍 Frankfurt | 📆 April 12–20, 2026

Our colleagues Vinith Kishore and Valentin Debarnot from the @ivandokmanic.bsky.social lab have come up with an amazing deep learning tool for denoising and filling the missing wedge in #cryoET data. I'm pleased to introduce Icecream🍧

Check out our preprint! With new molecular mechanisms, 140 subtomogram averages, and ~600 annotated cells under different conditions, we @embl.org were able to describe bacterial populations with in-cell #cryoET. And there’s a surprise at the end 🕵️ www.biorxiv.org/content/10.1... #teamtomo

More great cryo-ET benchmarking work from my colleagues Maarten Tuijtel, Tomáš Majtner, Beata Turoňová, & @becklab.bsky.social: Systematic study of how tilt increment choice affects downstream TM & STA. Preprint has the optimally balanced tilt increment. #TeamTomo www.biorxiv.org/content/10.1...

We’re hosting the @embo.org Practical Course on In-situ structural biology by CryoFIB and CryoET in April 2026! 🧬❄️ You can expect hands-on training, cutting-edge methods, and a chance to work alongside leading experts in structural biology. 🔗 Apply by Dec 8, 2025: meetings.embo.org/event/26-in-...

AI for Cryo-ET 🌟 Our new paper presents a deep-learning framework that cleans up faulty tilt series in a more automated, precise and powerful way! 💪 Read more here: www.sciencedirect.com/science/arti...

New from my colleagues Markus Schreiber & Beata Turoňová: TANGO, an #opensource tool for more holistic spatial analysis of cryo-ET particles. It can clean or cluster particles, analyze lattices, and identify patterns. #TeamTomo www.biorxiv.org/content/10.1...

Hey #TeamTomo, Ever been in need of a tutorial about the fundamentals of cryo-electron tomography? From preprocessing raw frames to high-res subtomogram averaging? That's why @florentwaltz.bsky.social and I made this website! tomoguide.github.io Follow the thread 1 /🧵 #CryoET #CryoEM 🔬🧪

🦠🧠 MemBrain update! 🧠🦠 We’ve updated our preprint! It now covers the full MemBrain v2 pipeline for end-to-end membrane analysis in #CryoET: segmentation, particle picking, and spatial statistics. 🔗 Preprint: doi.org/10.1101/2024... 🔗 Code: github.com/CellArchLab/... 🧵(1/6) #TeamTomo

Looking for a tool to measure membrane thicknesses in tomograms (#TeamTomo)? Or curious about thickness differences within and across organelles? Check out my fellow @becklab.bsky.social PhD student @deglushk.bsky.social's excellent preprint: www.biorxiv.org/content/10.1...

Also, check out @sergiocruzleon.bsky.social's post for a cool video summary about our new heterochromatin preprint.

Excited to share our latest work on nuclear pore complex scaffold integrity and its perturbation. Now out in Nature Cell Biology: rdcu.be/eg4i9 Great work by Reiya Taniguchi who just opened his own research group back in Japan! 🥳

Automated Removal of Corrupted Tilts in Cryo-Electron Tomography https://www.biorxiv.org/content/10.1101/2025.03.13.642992v1

Hello world!

Fresh out of the press! ✨Check out our new paper on the violent entry of the HIV capsid into the nuclear pore — and how it cracks the gate! 🤯 💥 Incredible work from @becklab.bsky.social and @hummerlab.bsky.social 🤩 More here: www.biophys.mpg.de/2842024/hivcapsid-cracks-the-npc?c=2011019 🔗 #teamTOMO

So happy that my main PhD project on HIV-1 nuclear entry is now out in Cell. I want to especially thank my supervisor Martin @becklab.bsky.social and our amazing collaborators @maziarheidari.bsky.social from @hummerlab.bsky.social, Vojtech Zila from the Kräusslich lab and many others.

cryoTIGER: Deep-Learning Based Tilt Interpolation Generator for Enhanced Reconstruction in Cryo Electron Tomography https://www.biorxiv.org/content/10.1101/2024.12.17.628939v1

Celebrating Dr. Huaipeng Xing from #molecularsociology on successfully defending his #PhD! 👏🥳 He studied the visualization of protein synthesis and folding inside human cells by #cryoET #teamTOMO. Congrats, Huaipeng, and best wishes for your future! 🤩

Do not miss our latest #cryoET work! 🕵️ Xing et al. present the dynamic architecture of TRiC in human cells! The study of this protein-folding chaperonin opens the door to understanding its dysfunction in diseases like cancer and neurodegeneration 🧠🧪 #teamTOMO @becklab.bsky.social

Are you already on track to apply to our #gradschool? 👣 This is a little map of our application process! We hope, you don´t get lost on the way 😊 If you want to start your #phdjourney with IMPRS-CBP #applynow Don’t miss the chance and apply by February 7th. 👉 imprs-cbp.mpg.de 👈 #imprs_cbp

Hello 🦋! We’re excited to announce that applications for our #PhDprogram open in TWO weeks! 🤯 Join our #IMPRS and take your first steps toward groundbreaking research in Cellular Biophysics. 📅 Mark your calendar & stay tuned: imprs-cbp.mpg.de #PhDPositions #GradSchool #MaxPlanckSociety

Faster and more accurate protein modeling with CryFold. By focusing on nearby points (Local Attention) and employing map masking, CryFold excels in generating models from challenging maps regions. It enables a more cost-efficient model building. 🧵 💾 github.com/SBQ-1999/Cry...