KPL ApS

KPL ApS

@kplaps.bsky.social

At the Cutting-Edge of Protein Innovation. Learn more at https://kplbio.com/ .

97 followers294 following18 posts

KPL ApS
@kplaps.bsky.social

What started at KPL is finally coming to fruition! Congrats to the team and all the co-authors ! @jespervolsen.bsky.social @jesperolsenlab.bsky.social

Tanveer Batth@tsbatth.bsky.social

🚀 We're very excited to announce KRysin! We have been working on this for a long time and our preprint is finally today on bioRxiv 🧵👇 www.biorxiv.org/content/10.6...

biorxiv.orgHigh-fidelity bispecific protease KRysin for deeper plasma proteomics and phosphoproteomicsBottom-up proteomics relies on efficient and reproducible proteolytic digestion. Yet trypsin, the most widely used protease, frequently misses cleavages, particularly near prolines, acidic and phospho...
KPL ApS
@kplaps.bsky.social

KPL ApS is heading to #ASMS2026 in San Diego ! 🔬 We'll be presenting two posters on KRysin, our latest R&D development! 📌 WP 613 – KRysin for PTM Analysis 📌 WP 092 – KRysin for Plasma Proteome Analysis Come find us! 👇 Wednesday, June 3 | 10:30 am – 2:30 pm #ASMS #Proteomics#TeamMassSpec

KPL ApS
@kplaps.bsky.social

🇫🇮🔬 We're at #FinnProt2026 in Espoo, Finland! Our CTO Cristina Hernandez Rollan is presenting a poster with the latest from our R&D pipeline 👀 Stop by to get an early look at what's next from KPL! #Proteomics #MassSpectrometry #TeamMassSpec

KPL ApS
@kplaps.bsky.social

🧪 Just launched: Trypsin/Lys-C Premium Mix 🚀 We found that with the right formulation, you can add Lys-C and Trypsin together instead of sequentially. So we made it 💡 ✅ One step ✅ Lowest missed cleavages in its class ✅ Fully recombinant 🪧Available now 🔗 kplbio.com/trypsinlysc/

KPL ApS
@kplaps.bsky.social

Introducing Trypsin RMS – our third product is here. Recombinant. Methylated. Sequencing-grade. The robust trypsin #proteomics labs need, without the batch variability of animal-derived alternatives. Learn more: kplbio.com/trypsin/ #TeamMassSpec #science #proteins #enzymes

KPL ApS
@kplaps.bsky.social

Trypsin is the workhorse of bottom-up #proteomics. But when was it first discovered? Lets try an emoji poll ! 😇 1836 🤭 1876 😜 1897 🤑 1931 Reply with your guess! Correct answer in the replies! #TeamMassSpec

KPL ApS
@kplaps.bsky.social

The answer is 🤭 , 1876! Wilhelm Kühne discovered trypsin at the University of Heidelberg. He also coined the term "enzyme" the following year. "Trypsin" comes from the Greek word "thrýpto" meaning "I break". 150 years later it's the most used protease in mass spec for breaking ... ahem proteins!

KPL ApS
@kplaps.bsky.social

Does enzyme quality matter in #proteomics? New study in JPR w/ @jesperolsenlab.bsky.social shows: LysC-Trypsin reduces missed cleavages from 30% → 15% KPL's A. lyticus LysC achieves >90% efficiency in systematic comparison. Congrats to the authors! pubs.acs.org/doi/10.1021/... #TeamMassSpec

pubs.acs.orgComparative Analysis of Lysine-Specific Peptidases for Optimizing Proteomics WorkflowsThis study presents a comparative analysis of three LysC endopeptidase homologues from Achromobacter lyticus (A. lyticus),Pseudomonas aeruginosa and Lysobacter enzymogenes for mass spectrometry-based proteomics. Utilizing a protein aggregation capture workflow with HeLa cell lysates, we assessed the enzymes’ cleavage specificity, digestion efficiency, and performance across various experimental conditions. Results showed that while all three LysC homologues exhibited high cleavage specificity at lysine residues, A. lyticus LysC outperformed the two others with superior peptide identification, digestion efficiency, and protein coverage, especially at shorter digestion times. Our experiments using a combination ofA. lyticusLysC and trypsin demonstrated the importance of employing LysC for significantly minimizing missed cleavage rates in tryptic digests, especially with regard to lysine-containing peptides. This study underscores A. lyticus LysC’s potential as an optimal choice for enhancing mass spectrometry-based proteomics.
KPL ApS
@kplaps.bsky.social

Happy Holidays from KPL! 🎄 Our first full year: 2 product launches, lots of foundation building, and countless hours pushing #proteomics forward. Our commitment: high quality, high performance, competitive prices. No compromises. 2026 is looking exciting - stay tuned! ✨

KPL ApS reposted

Shabaz Lab
@shabazlab.bsky.social

A basic solution to a very common problem. collab with @kusterlab.bsky.social and @bendavisgroup.bsky.social www.mcponline.org/article/S153...

mcponline.orgAddressing NHS chemistry: Efficient quenching of excess TMT reagent and reversing TMT-over labelling in proteomic samples by methylamineN-Hydroxysuccinimide (NHS) ester chemistry is used extensively across proteomics sample preparation. One of its increasingly prevalent applications is in isobaric reagent-based quantitation such as iT...

KPL ApS reposted

Shabaz Lab
@shabazlab.bsky.social

Shout out to the organisers @jesperolsenlab.bsky.social, (Tina Ludwig) @baybioms.bsky.social kathrynslilley.bsky.social, Caroline May and Britta Eggers and the speakers @bonalditiziana.bsky.social, @coscialab.bsky.social, @kusterlab.bsky.social, @anamdv.bsky.social, @jsandow.bsky.social

Shabaz Lab@shabazlab.bsky.social

The Brixen Proteomics Summer School speaker list has been finalised!. Lots of amazing science to be discussed! Big thanks to @eupaproteomics.bsky.social @msaid-de.bsky.social @thermofishersci.bsky.social @biognosys.bsky.social @ionopticks.bsky.social @evosep.bsky.social for support

KPL ApS
@kplaps.bsky.social

🎄 Wrapping up a amazing year at KPL! ✨As we celebrate this season, we're grateful for our wonderful customers and team. 🌟We look forward to making 2025 even more extraordinary! 🎉 Happy Holidays from our family to yours! ⭐️ #HappyHolidays #NewYear2025 #proteomics #TeamMassSpec

KPL ApS
@kplaps.bsky.social

#DAPSOC CelebrateMS is underway, starting with the honoring of Peter Roepstorff's contributions to #Proteomics and #TeamMassSpec . He made a surprise appearance to standing ovation!

KPL ApS
@kplaps.bsky.social

🚀Introducing Arg-C Zero (KPL0146)🧬 Next-gen proteomic enzyme with close to 0% missed cleavages at Arginine residues! 🔬100% Arginine cleavage + ultimate specificity = next-level protein analysis magic ✨ 🧬 Details: kplbio.com/product/arg-c/ #Proteomics #TeamMassSpec #proteins

kplbio.comArg-C Zero - High-Fidelity Protease for Mass Spectrometry | KPL BioDiscover Arg-C Zero [KPL0146], a high-fidelity arginyl endopeptidase delivering 0% missed cleavages. Ideal for proteomics, Arg-C Zero ensures complete digestion, high purity, and compatibility with ma...

KPL ApS reposted

Devin Schweppe
@dschweppe.bsky.social

That’s a wrap, we’ve uploaded our version of record for the large scale PISA work led be Jon & co. to @elife.bsky.social : elifesciences.org/articles/95595 Thanks to the reviewers for helping us improve the manuscript, enjoyed the process of developing this resource.

elifesciences.orgLarge-scale characterization of drug mechanism of action using proteome-wide thermal shift assaysAn approachable framework for the scalable implementation of proteome-wide thermal shift assays to assess drug mechanisms of action.

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