Joe Dillard

Joe Dillard

@sacculus.bsky.social

Bacteriologist. We study Nesseria, Gardnerella, and all things related to peptidoglycan. Professor at UW-Madison.

885 followers639 following43 posts

Joe Dillard reposted

Joe Dillard reposted

The #WHO just released recommendation of #doxyPEP for #STI prevention among MSM and transgender women Monitoring of #AMR also recommended Research gaps still remain, including long-term impacts on AMR/microbiome and effectiveness in different settings www.who.int/teams/global... #STIsky

who.intDoxycycline PEPThis page provides technical information supporting the WHO guideline on the use of doxycycline post-exposure prophylaxis (PEP) for the prevention of bacterial STIs among men who have sex with men and...

Joe Dillard reposted

We’re hiring! Join the Weber Lab at the University of Iowa to study Chlamydia effector biology, centrosome regulation, vesicular trafficking, and innate immunity. Please share!

Joe Dillard reposted

Joe Dillard reposted

Exciting research here on the development and lab evaluation of #CRISPR #POC tests for #STIs (chlamydia, gonorrhea, syphilis, HSV 1 /2) Also very interesting they could detect S91F to predict cipro resistance in #gonorrhea POC tests for STIs can be game-changers in many settings #STIsky #IDsky

The Lancet Microbe@lancetmicrobe.bsky.social

New research article CRISPR-Cas-based diagnostics for point-of-care detection of sexually transmitted infections: a laboratory development and evaluation study www.thelancet.com/journals/lan... #IDSky #ClinMicro #STI #OpenAccess #OA

Dr. Cole's presentations at the Neisseria meetings were delightful. So sorry he is gone.

It is with deep sadness that we share the passing of Professor Jeff Cole. An esteemed academic, Jeffs research on bacterial adaptation to oxygen and nitrosative stress made a lasting impact on the field. He mentored nearly 60 doctoral students, our thoughts are with his family, friends & colleagues

Joe Dillard reposted

This is a great @cidjournal.bsky.social supplement for all you #gonorrhea, #STI, and #AMR enthusiasts. Or for anyone who’s heard about drug-resistant gonorrhea and wants to learn more. Will link articles in 🧵 below - check it out and share! #openaccess #IDsky #STIsky bsky.app/profile/carb...

CARB-X@carb-x.bsky.social

📘 New publication supported by CARB-X! A new supplement in Oxford University Press Clinical Infectious Diseases highlights why treatment diversification is essential to address rising drug resistance in N. gonorrhoeae. #IDSky 🧪 🔗 Read here: bit.ly/45KVg90

Joe Dillard reposted

Joe Dillard reposted

Joe Dillard reposted

STD cases have fallen, but not syphilis in newborns. Data for 2024 from the CDC showed a 3rd consecutive year of fewer gonorrhea cases & the 2nd year of fewer adult cases of chlamydia & the most infectious form of syphilis. However, congenital syphilis cases aren't seeing the same improvements👇

medpagetoday.comSTD Cases Fall, but Not Syphilis in NewbornsOverall decreases may be due to a general decline in young people having new partners

Joe Dillard reposted

Our paper describing transformation of Gardnerella species is published in Infection and Immunity. We think this will lead to further molecular studies of bacterial vaginosis and potential therapeutics. journals.asm.org/doi/10.1128/...

journals.asm.orgGenetic transformation of Gardnerella species and characterization of vaginolysin and sialidase mutants | Infection and ImmunityGenetic manipulation is a fundamental process in the study of bacterial pathogenesis. The ability to make mutations and complements is necessary for definitive identification of virulence genes, and the ability to make unmarked mutations is preferable in these studies (1). Certain bacterial pathogens were considered genetically intractable for many years, such as Mycobacterium tuberculosis or Chlamydia trachomatis, and studies of their virulence mechanisms necessarily focused on epidemiology, immunology, cell biology in the host, and proteomic or transcriptomic analyses (2, 3). Those classic cases were eventually resolved by the discovery of effective transduction, natural transformation, and chemical transformation methods, greatly facilitating the identification of virulence factors (4–7). Gardnerella species have similarly been considered genetically intractable, and like M. tuberculosis and C. trachomatis, Gardnerella have an unusual cell envelope. Similar to other Actinobacteria, Gardnerella are generally considered gram-positive, but stain gram-variable because of their unusual and incompletely characterized envelope (8).

Joe Dillard reposted

Joe Dillard reposted

Joe Dillard reposted

Thrilled to share the first preprint from my group! Prevotella are predominant but understudied members of the respiratory microbiome. They have a reputation for being hard to work with, but understanding them is likely important for understanding how they contribute to respiratory health. 🧫🔬

bioRxiv Microbiology@biorxiv-microbiol.bsky.social

Development of a basal media for carbon utilization assay and analysis of the starch utilization locus in Prevotella melaninogenica https://www.biorxiv.org/content/10.1101/2025.07.04.663206v1

I wrote a preview for Cell Host & Microbe about the article from Kurt-Jones et al. on IFN-epsilon enhancement of gonococcal infection. One cool facet of this work is that it provides a possible mechanism for Chlamydia to increase gonococcal co-infection. www.cell.com/cell-host-mi...

cell.comType I interferons give gonococci a sweet treat and a chance at survivalIn this issue, Kurt-Jones et al. demonstrate a surprising role for the type I interferon, IFN-ε, in Neisseria gonorrhoeae infections of the female reproductive tract. Rather than protecting the host, ...

Joe Dillard reposted

To get DNA constructs across the actinobacterial envelope, we grew the bacteria with a cell wall-weakening agent, D-cycloserine. To protect the DNA, we needed to methylate it, and circular DNA worked better than linear. So, suicide plasmids worked.

Being geneticists, we also needed to complement. We built a complementation construct that directs introduced DNA to a region on the chromosome that is conserved among Gardnerella species. I think these tools should be useful for the whole Gardnerella community.

To get DNA constructs across the actinobacterial envelope, we grew the bacteria with a cell wall-weakening agent, D-cycloserine. To protect the DNA, we needed to methylate it, and circular DNA worked better than linear. So, suicide plasmids worked.

Finally, Amy made a plasmid that had mutated pheS as a counter-selectable marker. So, we were able to deliver mutation constructs to the chromosome by selecting for the plasmid to integrate and then selecting for it to resolve. The isolates could be screened to see if they kept the mutation.

I heard that Dr. Kim Jefferson's lab at VCU was doing oligo recombineering in Gardnerella, and she was nice enough to collaborate with us. Then Dr. Erin Garcia who was doing that work, came to post-doc with us. Erin taught us to electroporate Gardnerella with oligos.

The oligo work was nice, but we wanted to use selectable markers to knock-out virulence and colonization genes. Amy Klimowicz tried many different methods to make electroporation work better, to protect the DNA from degradation, and then to get insertions in the chromosome.

Checkout out our preprint describing mutagenesis methods for Gardnerellla species. We showed that a vaginolysin mutant was defective in lysing cervical cells in human cervix explants and that a sialidase mutant was deficient in degrading human cervical mucus. www.biorxiv.org/content/10.1...

biorxiv.orgGenetic transformation of Gardnerella species and characterization of vaginolysin and sialidase mutantsBacterial vaginosis (BV) is the most prevalent vaginal disorder in women of childbearing age and causes pregnancy complications including preterm birth. Species of Gardnerella increase just prior to t...

We've worked on Neisseria gonorrhoeae for decades. After an interesting session at the Neisseria meeting in Asilomar, I decided we needed to understand more about the other bacteria that gonococci interact with. Gardnerella was an obvious choice, since BV patients are more likely to get gonorrhea.

We have developed methods for making targeted mutations in Gardnerella species. It's been a long slog, and the bacteria put up a lot of hurdles. Thanks to the hard work and ingenuity of Amy Klimowicz and Erin Garcia, we can make deletions, point mutations, and complements. Preprint is on bioRxiv.

Joe Dillard reposted

Our review on Z-ring placement mechanisms in cocci is out! This was a fun one to write! #Microsky 🦠🧫

Kumaran Ramamurthi@ramamurthilab.bsky.social

Cell division in rod-shaped bacteria is well studied (think "Min system"), but how do round bacteria find their middle? @felixrl.bsky.social 's new review article covers how #Staph aureus, Streptococci, and others figure out how to perform binary fission. portlandpress.com/biochemsoctr...

Joe Dillard reposted

So…um…who is doing the testing for multi drug resistant gonorrhea and resistant trichomonas ????

Alexander Tin@alexander-a-tin.bsky.social

Labs at CDC investigating STD and hepatitis outbreaks have now been cut, as part of Secy Kennedy's layoffs Specimens were still arriving from state health departments, with no one left on job from labs to handle them "The outbreak detection is gone" www.cbsnews.com/news/rfk-jr-...

Joe Dillard reposted

Joe Dillard reposted

I’m very happy to share our latest work on the ParABS system. We investigated the dynamic interaction between the ATPase ParA and the CTPase ParB mediating chromosome segregation in Myxococcus xanthus. I would like to thank all the people involved.

bioRxiv Microbiology@biorxiv-microbiol.bsky.social

Molecular basis of ParA ATPase activation by the CTPase ParB during bacterial chromosome segregation https://www.biorxiv.org/content/10.1101/2025.03.14.642875v1

Joe Dillard reposted

Joe Dillard reposted

Europe reports surging STIs, including #AMR gonorrhea, alongside FDA approval of a new antibiotic and WHO’s AMR research priorities, stressing urgent global action. Related updates (mpox, dengue, COVID) underscore need for coordinated innovation and stewardship. www.cidrap.umn.edu/sexually-tra...

cidrap.umn.eduSexually transmitted infections continue to rise in EuropeThe rate of gonorrhea cases across Europe rose by 31% in 2023 compared with 2022, and is up 321% since 2014.

Joe Dillard reposted