Salil Bidaye

Salil Bidaye

@salilbidaye.bsky.social

Neuroscientist, Research Group Leader @mpfneuro.bsky.social interested in how animals move, especially if they have 6 legs. Formerly @ucberkeleyofficial.bsky.social @impvienna.bsky.social https://mpfi.org/our-labs/bidaye-lab/

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Here’s our latest preprint on uncovering neural control of a coordinated walking pattern! doi.org/10.64898/202... (author-list jumbled, see pdf for corrected). Video shows the central paradigm in this study, we optogenetically induce walking in headless flies and sequentially reduce proprioception.

Salil Bidaye reposted

Salil Bidaye reposted

a good summary of how we got here and what it means. i would add is that everyone who works with the fly connectome datasets knows that they all far from "complete". each one, though monumental, has major reconstruction/annotation issues, not to mention the lack of a peripheral nervous system.

Patrick Mineault@patrickmineault.bsky.social

Are virtual flies playing Beat Saber? SM64? Doom? No. The new fly connectome is an incredible achievement! Less is going on in the sim videos than it seems. I'm stoked to see people engage with neuroscience though. My breakdown: www.neuroai.science/p/are-flies-...

Salil Bidaye reposted

This article is so frustrating to read. First, this is not just “google’s brain” - there has been so much effort made by scientists around the world to get these connectomes to where they are today, and none of that is recognized here, instead it’s all turned into “big tech company 👍🏻”. And second -

Eurogamer@eurogamer.bsky.social

"The more aroused the fly is the harder it tries to shoot" - Google's fully mapped fly brain is now playing Doom, Beat Saber, and more. https://bit.ly/46l7s0l

Salil Bidaye reposted

Salil Bidaye reposted

Salil Bidaye reposted

"The smallest organisms may hold the biggest answers." Here, two new studies on the fruit fly connectome led by Tuthill, Brunton, and Bidaye address the mechanisms underlying walking. It's well summarized by a piece in the Transmitter: www.thetransmitter.org/systems-neur...

thetransmitter.orgLong-sought walking circuit found in fruit fliesThe neuronal circuit controlling repetitive locomotion patterns in any animal has been a mystery until now.

Our recent work covered in this cool piece by @natmesanash.bsky.social @thetransmitter.bsky.social . Thanks for using our videos!

The Transmitter @thetransmitter.bsky.social

Researchers have long searched for the neural circuit underlying locomotion. Now they’ve found it in flies. By @natmesanash.bsky.social #neuroskyence www.thetransmitter.org/systems-neur...

Here’s our latest preprint on uncovering neural control of a coordinated walking pattern! doi.org/10.64898/202... (author-list jumbled, see pdf for corrected). Video shows the central paradigm in this study, we optogenetically induce walking in headless flies and sequentially reduce proprioception.

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Here’s our latest preprint on uncovering neural control of a coordinated walking pattern! doi.org/10.64898/202... (author-list jumbled, see pdf for corrected). Video shows the central paradigm in this study, we optogenetically induce walking in headless flies and sequentially reduce proprioception.

View full thread

If you're still reading, you should see the preprint and you will realize the mammoth of data and analysis that went into this. All led by an incredible shared first-authors team of Neha Sapkal and Divya S Kumar. Couldn't be prouder and luckier to have this level of expertise in the lab!

Here’s our latest preprint on uncovering neural control of a coordinated walking pattern! doi.org/10.64898/202... (author-list jumbled, see pdf for corrected). Video shows the central paradigm in this study, we optogenetically induce walking in headless flies and sequentially reduce proprioception.

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In doing so we propose a framework by which brain commands can flexibly modulate the inter-leg couplings in a task-specific manner. Increase central-couplings when walking fast forward and decrease couplings when walking slow forward or backward and let proprioception take the lead

This final point provides a circuit basis for a central concept underlying walking gaits in all animals. A mountain of work from Ansgar Büschges (stick insects), Einat Fuchs, Phil Holmes et al (cockroaches) and many others have shown inter-leg couplings vary across speed and across species.

Here’s our latest preprint on uncovering neural control of a coordinated walking pattern! doi.org/10.64898/202... (author-list jumbled, see pdf for corrected). Video shows the central paradigm in this study, we optogenetically induce walking in headless flies and sequentially reduce proprioception.

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We could also find ways to slow down and speed up the stepping rhythm (co-activate multiple DNs!) and in doing so ended up discovering a new mechanism for acceleration which for obvious reasons we call as a front-wheel-drive (FWD) mechanism.

Most importantly, we used a kinematics-guided connectome search to isolate candidate circuits. We found leg-specific CPGs and inter-leg coupling motifs to explain the kinematics data. Check the preprint for these cool motifs too heavy for uploading. Here's one motif for let-right alternation.

Here’s our latest preprint on uncovering neural control of a coordinated walking pattern! doi.org/10.64898/202... (author-list jumbled, see pdf for corrected). Video shows the central paradigm in this study, we optogenetically induce walking in headless flies and sequentially reduce proprioception.

View full thread

3D leg kinematics allows us to analyze the nitty-gritties of every stepping leg and we could see how proprioception shapes centrally generated smooth sinusoidal-like kinematics into structured “real-steps” with a stance and swing period.

We then looked at inter-leg coordination. Remarkably DNg100 drove the 6 legs to step with amazing inter-leg coordination. See the tripod like stepping even in highly proprioception deficient animals (headless fly with tiny stumps for legs!). Other neurons like MDN do not drive inter-leg coupling!

Here’s our latest preprint on uncovering neural control of a coordinated walking pattern! doi.org/10.64898/202... (author-list jumbled, see pdf for corrected). Video shows the central paradigm in this study, we optogenetically induce walking in headless flies and sequentially reduce proprioception.

View full thread

We can stimulate headless walking by activating severed axons of 3 descending neurons identified in our previous work. Moonwalker neurons (MDN, from my PhD work) drive backward walking whereas DNg100 (BDN2) and DNg97(oDN1) discovered in previous work from my lab, drive forward walking.

In proprioception deficient conditions DNg100 still drives stepping across the 6 legs of the fly, whereas DNg97 predominantly drives front leg stepping and MDN drives hind leg stepping. This shows evidence of modular leg-specific CPGs.

Here’s our latest preprint on uncovering neural control of a coordinated walking pattern! doi.org/10.64898/202... (author-list jumbled, see pdf for corrected). Video shows the central paradigm in this study, we optogenetically induce walking in headless flies and sequentially reduce proprioception.

When animals walk there are 2 rhythmic signals in the spinal or ventral nerve cords. CPG generated central rhythm and rhythmic proprioceptive feedback. This paradigm lets us disentangle these two sources and obtain mechanistic insights into walking pattern generation. Here are just a few highlights

Max Planck PhD program deadline on Dec 1. I’m hiring through this. Shoot a DM if you have any questions.

Looking for a cutting-edge neuroscience PhD program? IMPRS Alum, Professor Akshay Naraine, says choose one that aligns with your values: "It makes the tough days easier & keeps you satisfied long term." Apply for our IMPRS for Synapses and Circuits PhD program through Dec. 1: bit.ly/4hBHV7P

Hey spinal/ventral nerve cord folks. we’re organizing this cross species “cords” meeting @hhmijanelia.bsky.social . Hurry up and upload your abstracts. As a trainee these were my favorite meetings (small and focused) where I got the most useful feedback. DM in case of any Qs.

Janelia Research Campus@hhmijanelia.bsky.social

📢 Applications are now open for our cross-disciplinary conference on nerve cord circuits. 🧠 Join experts to probe the organization & function of cord circuits across species. 🏨 Hotel, meals & registration covered. 🗓️ Apply by May 27 @ janelia.news/CRD25 @hhmi.org @salilbidaye.bsky.social

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