Sam Daly

Sam Daly

@samofmidworld.bsky.social

music nerd, book absorber, microscope builder | postdoc @ Gershlick Lab and TheLeeLab, Cambridge and aspiring communicator | they/them/their 🏳️‍⚧️ ORCID: 0000-0002-8559-3161

570 followers530 following30 posts

Sam Daly reposted

LGBTQ+ STEM
@lgbtqstem.bsky.social

Look out for this event! Focussed on creating change and trading experiences and ideas about positive research culture for LGBTQ+ people working in STEM.

Queer in STEM Workshop@queerstemworkshop.bsky.social

Registration is now open for the LGBTQ+ in STEM Workshop! 22nd-23rd September, Cambridge. Exciting speaker line-up, with subsidised accommodation and travel bursaries. Come along, share your experiences of being queer in STEM www.outsavvy.com/event/35459/...

Sam Daly
@samofmidworld.bsky.social

Excited to share some recent work tracking the 3D movement of individual molecules inside the organelles of living cells (💡+ 🦠 + ⚽️💨) with the best team @joechambers.bsky.social @jamesdmanton.bsky.social @jsbeckwith.bsky.social @dgershlick.bsky.social @stevethechemist.bsky.social

bioRxiv Biophysics@biorxiv-biophys.bsky.social

Volumetric single-molecule tracking inside subcellular structures https://www.biorxiv.org/content/10.1101/2025.06.09.658280v1

View full thread
View full thread
Sam Daly
@samofmidworld.bsky.social

We're now well into the second session of the first day, looking at further method development! We're listening to James Simon who is discussing the use of conductance microscopy in battery research, and Stefan Oswald on tracking battery ions with microscopy! #CMM2025

Sam Daly reposted

Natural History Museum, London
@nhm-london.bsky.social

We’re thrilled to announce our exhibition, Space: Could Life Exist Beyond Earth?, opening 16 May 2025. 🚀 Rooted in the latest cutting-edge scientific research from the Museum, the exhibition will uncover evidence that life could exist beyond our planet.

Sam Daly reposted

Zach Marin
@zacsimile.bsky.social

By putting a donut between our faces and each article we read, we were able to extract more information than ever before about MINFLUX. You can read the new review article from @maxperutzlabs.bsky.social by Lukas Scheiderer, myself, and @jonasries.bsky.social at www.nature.com/articles/s41...

nature.comMINFLUX achieves molecular resolution with minimal photons - Nature PhotonicsThe authors review MINFLUX super-resolution microscopy, outlining its advantages and limitations, recent progress, and an outlook for future developments.
Sam Daly
@samofmidworld.bsky.social

Excited to see that my first, first author paper out of my PhD was selected among the Top 25 Physics Research papers of 2024 by Nature Communications! @naturecomms.bsky.social www.nature.com/articles/s41...

nature.comHigh-density volumetric super-resolution microscopy - Nature CommunicationsCurrent approaches for volumetric super-resolution microscopy can yield large and complex PSF spatial footprints. Here, the authors show a super-resolution microscopy approach using a hexagonal microl...
Sam Daly
@samofmidworld.bsky.social

If you're interested in advanced 3D super-resolution microscopy then there is an exciting new job opportunity in TheLeeLab here in Cambridge. Happy to answer any questions! 🔬🧪🦠 www.jobs.cam.ac.uk/job/50547/

jobs.cam.ac.ukPostdoctoral Research Associate in Single-Molecule Imaging and Super-Resolution Microscopy (Fixed Term) - Job Opportunities - University of CambridgePostdoctoral Research Associate in Single-Molecule Imaging and Super-Resolution Microscopy (Fixed Term) in the Yusuf Hamied Department of Chemistry at the University of Cambridge.
Sam Daly
@samofmidworld.bsky.social

First paper out of my postdoc has just dropped! We map the 3D spatial relationship of PSD95 in mouse brain using DHPSF super-resolution microscopy… and in just enough time for the last #FluorescenceFriday before Christmas! 🎄 pubs.acs.org/doi/10.1021/...

pubs.acs.org3D Super-Resolution Imaging of PSD95 Reveals an Abundance of Diffuse Protein Supercomplexes in the Mouse BrainPSD95 is an abundant scaffolding protein that assembles multiprotein complexes controlling synaptic physiology and behavior. Confocal microscopy has previously shown that PSD95 is enriched in the postsynaptic terminals of excitatory synapses and two-dimensional (2D) super-resolution microscopy further revealed that it forms nanoclusters. In this study, we utilized three-dimensional (3D) super-resolution microscopy to examine the nanoarchitecture of PSD95 in the mouse brain, characterizing the spatial arrangement of over 8 million molecules. While we were able to identify molecular arrangements that have been previously reported, imaging in 3D allowed us to classify these with higher accuracy. Furthermore, 3D super-resolution microscopy enabled the quantification of protein levels, revealing that an abundance of PSD95 molecules existed outside of synapses as a diffuse population of supercomplexes, containing multiple copies of PSD95. Further analysis of the supercomplexes containing two units identified two populations: one that had PSD95 molecules separated by 39 ± 2 nm, and a second with a separation of 94 ± 27 nm. The finding that there exists supercomplexes containing two PSD95 units outside of the synapse suggests that supercomplexes containing multiple protein copies assemble outside the synapse and then integrate into the synapse to form a supramolecular nanocluster architecture.

Sam Daly reposted

Jonny Nixon-Abell
@abelljonny.bsky.social

Hullooooo Cell Biology friends. If you're heading to #CellBio2024 in (hopefully!) sunny San Diego this year, then come and check out our session on 'ER structure and function across cell types' Room28C- Dec18th 09:00am! See you there! **Please RT** 🥳

Sam Daly
@samofmidworld.bsky.social

Exciting new preprint just dropped by the Lee lab! Using 3D super-resolution microscopy to map the distribution of protein PSD95 in brain tissue. www.biorxiv.org/content/10.1...

biorxiv.org3D super-resolution imaging of PSD95 reveals an abundance of diffuse protein supercomplexes in the mouse brainPSD95 is an abundant scaffolding protein that assembles multiprotein complexes controlling synaptic physiology and behavior. Confocal microscopy has previously shown that PSD95 is enriched in the post...

You are up to date.