SinghLab@SNIoE

SinghLab@SNIoE

@singhlab.bsky.social

Regenerative & Stress Biology Lab, Shiv Nadar University, Delhi-NCR, India Regenerative Plasticity | Zebrafish | In vivo Fluorescent Imaging | Liver Regeneration | Starvation Response | Alcoholic Liver Disease

898 followers1.2K following61 posts

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SinghLab@SNIoE
@singhlab.bsky.social

🚨 Just out in Nature Communications: Cholangiocytes contribute to hepatocyte regeneration after partial #liver #injury during growth in #zebrafish We challenge old assumptions on liver regeneration! 🔗 nature.com/articles/s41... 🧵(1/8)

nature.comCholangiocytes contribute to hepatocyte regeneration after partial liver injury during growth spurt in zebrafish - Nature CommunicationsDuring partial liver injury in growing zebrafish, cholangiocytes regenerate hepatocytes through transdifferentiation, revealing a stage-specific regeneration mechanism regulated by mTORC1 signaling.
SinghLab@SNIoE
@singhlab.bsky.social

As part of the Faculty Development Program at Shiv Nadar University , received these 10 commandments for faculty. This is the best advice I have seen, and certainly hits the nail on the head. Especially about focusing and getting the first study out early on.

SinghLab@SNIoE
@singhlab.bsky.social

If you’re interested in liver regeneration, cholangiocyte biology, or cell fate plasticity, we’d love to hear your thoughts! #LiverRegeneration #Zebrafish #RegenerativeBiology #CellPlasticity (8/8)

SinghLab@SNIoE
@singhlab.bsky.social

This reveals that cholangiocyte plasticity is not just a backup plan, but an active, physiological response during liver growth and moderate injury. A new paradigm: transdifferentiation can occur without senescence, fibrosis, or chronic damage. (5/8)

SinghLab@SNIoE
@singhlab.bsky.social

Enter CellCousin: a new zebrafish tool we designed to trace liver regeneration. 🟢 Spared hepatocytes 🔴 Ablated hepatocytes 🔵 De novo hepatocytes (not hepatocytes before injury) This lets us precisely map where new hepatocytes come from after injury. (3/8)

SinghLab@SNIoE
@singhlab.bsky.social

Surprisingly, de novo hepatocytes emerged even when spared ones were present—a key deviation from the idea that cholangiocytes only activate after massive hepatocyte loss. We confirmed this with a 17% partial hepatectomy model. ✂️🐟 (4/8)

SinghLab@SNIoE
@singhlab.bsky.social

The liver can regenerate itself—a fact known since the myth of Prometheus. But when hepatocytes are damaged, can cholangiocytes step in to rebuild the liver even if some hepatocytes remain? We built a new zebrafish model to test it. (2/8)

SinghLab@SNIoE reposted

Filipa Simões
@simoesfilipa.bsky.social

🫀Job alert 🧬 come and be our colleague! Join a BBSRC-funded consortium aiming to understand how the human heart develops - you will be contributing multimodal single cell & spatial transcriptomics data using zebrafish and human cardiac organoids as models. Learn more: www.heartdevelopment.org (1/4)

heartdevelopment.orgCellTalk HHD | Human Heart Development ResearchCellTalk-Human Heart Development is a BBSRC funded consortium established to find out more about how cells come together to build the heart as it grows inside the womb.
SinghLab@SNIoE
@singhlab.bsky.social

Pleased to have contributed commentary to this recent article by Maria Bolevich for Interesting Engineering, which explores the science of tissue #regeneration and its potential applications in medicine. Read the full article here: interestingengineering.com/health/body-...

interestingengineering.comWhy the body's natural regeneration isn't enough to stop chronic diseaseFrom skin renewal to liver repair, the body is rebuilding itself. Scientists are now uncovering why some organs heal and others don’t.
SinghLab@SNIoE
@singhlab.bsky.social

How does DDCreER work? We fused CreER to a destabilizing domain (DD) that is degraded unless stabilized by TMP. 💊 TMP = protein stabilization 🟣 4-OHT = nuclear translocation Only when both are present does recombination occur—ensuring tight temporal control.

SinghLab@SNIoE
@singhlab.bsky.social

What is CellCousin? It uses stochastic recombination and NTR-based ablation to remove only a subset of hepatocytes. 🟢 Spared cells 🔵 New (De novo) hepatocytes This allows direct comparison of lineage contributions during liver repair.

SinghLab@SNIoE
@singhlab.bsky.social

What’s new in CellCousin2? ✅ DDCreER: chemically inducible Cre with low leakiness ✅ NTR2.0: ablation with 10× less metronidazole ✅ Cleaner, more precise regeneration readouts The system is modular and adaptable to other tissues or models.

SinghLab@SNIoE reposted

Carol Yang
@yhcarolyang.bsky.social

Interested in understanding how neural-pancreas interplay contributes to blood sugar control? We have an exciting PhD project to study galanin regulation of pancreatic #islet hormone secretion in live #zebrafish www.exeter.ac.uk/research/ins...

LSI-Exeter@lsiexeter.bsky.social

Be part of the great PhD community at the Living Systems Institute, University Exeter @exeter.ac.uk. Applications for LSI-funded PhD studentships are open until Monday, 9th June 2025. Apply now! www.exeter.ac.uk/research/ins...

SinghLab@SNIoE
@singhlab.bsky.social

We celebrated a big milestone in our lab: our first #Undergrad intern presented her work at lab meeting! She helped develop two new #liver #injury models, and is on her way to becoming a fantastic scientist. Thanks to PhD student Garnik for mentoring her so well! 👏🧪 #TeamScience

SinghLab@SNIoE
@singhlab.bsky.social

I'm excited to share that I will join School of Natural Sciences, Shiv Nadar University as an Associate Professor! Looking forward to working with amazing students and being part of a thriving research community.

SinghLab@SNIoE reposted

Zebrafish Rock! 🎃
@zebrafishrock.bsky.social

Come help us build an inclusive community, for those finterested! Did they mention it’s FREE?!? 🧪

International Zebrafish Society@izfs.bsky.social

The first IZFS and ZDMS joint webinar promoting global awareness about our varied backgrounds and experiences in science takes place tomorrow, March 6th at 10am EST! Featuring speaker Dr. Rachel Brewster, you will not want to miss this! Register here today: forms.gle/5uWMpdspRhtz...

forms.gleJoint DEI session IZFS/ZDMSDoes this form look suspicious? Report

SinghLab@SNIoE reposted

Wehner lab | Neuroregeneration
@wehnerlab.bsky.social

Replying to @wehnerlab.bsky.social

We demonstrate that this biphasic regulation limits the neutrophil-driven acute inflammatory phase, preventing chronic inflammation, fostering a lesion environment with mechano-structural properties conducive to regeneration.

SinghLab@SNIoE reposted

Wehner lab | Neuroregeneration
@wehnerlab.bsky.social

Excited to share the latest work from our lab: www.biorxiv.org/content/10.1...

biorxiv.orgBiphasic inflammation control by dedifferentiated fibroblasts enables axon regeneration after spinal cord injury in zebrafishFibrosis and persistent inflammation are interconnected processes that inhibit axon regeneration in the mammalian central nervous system (CNS). In zebrafish, by contrast, fibroblast-derived extracellu...

SinghLab@SNIoE reposted

Valerie Tornini
@valetornini.bsky.social

We are openly recruiting at all levels at the Tornini Lab at the University of California, Los Angeles (UCLA)! Please share with your networks. For more information, please check the info below, visit www.torninilab.org, or email me directly at vtornini at ucla dot edu. Thank you in advance!

SinghLab@SNIoE reposted

Sofia de Oliveira, Ph.D.
@sdeoliveira.bsky.social

Our debut here 🥰 Non-invasive intravital imaging of wound in skin epithelium of a juvenille zebrafish fed with a High-Cholesterol diet (HCD) versus a Normal Diet (ND) Zebrafish is an outstanding model to study how diet and metabolic disease impact neutrophils Keep following us, spread the word!

SinghLab@SNIoE
@singhlab.bsky.social

Finally, our work underscores the power of #zebrafish as a model for studying cellular plasticity and #regeneration. From developing new tools like CellCousin to exploring fundamental biology, this system is transformative for liver research. 🐟🔬 (9/12)

SinghLab@SNIoE
@singhlab.bsky.social

Our findings suggest that cholangiocyte-to-hepatocyte transdifferentiation isn’t just a backup plan—it’s an active, physiological response during liver repair during growth. 💡(7/12)

SinghLab@SNIoE
@singhlab.bsky.social

This challenges the current paradigm of liver regeneration, showing that cholangiocyte transdifferentiation, previously considered secondary to hepatocyte self-duplication, can occur independently of senescence or fibrosis—unlike its role in chronic liver disease (CLD). 🌟(8/12)

SinghLab@SNIoE
@singhlab.bsky.social

Surprisingly, we found that de novo hepatocytes emerged even when spared hepatocytes were present. This challenges the prevailing idea that cholangiocyte-driven regeneration only kicks in after severe hepatocyte loss. 🤔 (5/12)

SinghLab@SNIoE
@singhlab.bsky.social

To validate these findings, we added a partial #hepatectomy (PHx) model—removing 17% of liver mass. Again, de novo hepatocytes emerged, highlighting cholangiocyte plasticity as a key regenerative mechanism, even after moderate injuries. ✂️🩺 (6/12)

SinghLab@SNIoE
@singhlab.bsky.social

Using #zebrafish, a powerful model for studying liver biology due to its transparency and genetic tractability, we developed CellCousin—a system for lineage tracing and quantifying hepatocyte regeneration after injury. (3/12)

SinghLab@SNIoE
@singhlab.bsky.social

Here's how CellCousin works: Hepatocytes are stochastically labeled green (spared) or red (ablated). Upon injury, any blue-labeled hepatocytes that emerge are de novo—they weren’t hepatocytes before. This gives us a clear picture of regeneration dynamics. 🎨🐟 (4/12)

SinghLab@SNIoE
@singhlab.bsky.social

The liver’s regenerative capacity is legendary—think Prometheus. 🔥 But what happens when hepatocytes can't do the heavy lifting? Enter cholangiocytes, which can transdifferentiate into hepatocytes under certain conditions. But how? Why? And when? (2/12)

SinghLab@SNIoE reposted

Nikolai Slavov
@slavov-n.bsky.social

5,562 proteins were found to have tissue-specific enrichment across 32 human tissues. The majority of them (3,088) are enriched at the protein but not RNA level. The brain hosts the largest number of proteins that are only enriched at the protein level but not RNA level.

SinghLab@SNIoE
@singhlab.bsky.social

What shapes the #immune profile of organs? Study from Ninov Lab #CRTD, led by Nadeem, find that developmental #celldeath define the immune cells of the pancreatic #islet in #zebrafish ! 🎉👏 Read more @embojournal.bsky.social : embopress.org/doi/full/10.... #Diabetes

embopress.orgDevelopmental beta-cell death orchestrates the islet’s inflammatory milieu by regulating immune system crosstalk | The EMBO JournalimageimageDuring islet development, beta-cell death, rather than proliferation alone, regulates beta-cell mass and shapes the immune environment. This process triggers immune system crosstalk, influen...

SinghLab@SNIoE reposted

Phil Campbell
@pdcampbell.bsky.social

Replying to @pdcampbell.bsky.social

We are looking to hire at all levels - undergrads, research technicians, graduate students, postdocs. Experience with zebrafish, induced pluripotent stem cell models, molecular genetic approaches, imaging, or programming is desirable but not required.