Viroscope

Actin 🩵 , Tubulin 🩷, DNA 🧡 for this #FluorescenceFriday. Highly confluent cells are chaotic, but that means there is always something new to notice when you look at the picture again! Different shapes, sizes and so much biology all in one small crowded space 😄 🔬: Joe McKellar, Viroscope Imaging

We get to work with so many cool cell models: Primary, immortalized, transformed... Brain, lung, liver... Human, mouse, reptile... But HEK... ...293Ts can be pretty cool too! 😉 💜 Purple: Actin 💛 Yellow: Ectopically expressed protein 🤍 White: DNA 🔬: Joe McKellar #FluorescenceFriday #SciArt

Nuclear Stars and Viral Auroras ✨ Red-hot nuclear constellations are frozen inside grey microcosmic vaults, while a bright viral aurora washes across the cytoplasm 🔴|🟠: Mx1-L7A 🔵: Influenza A virus nucleoprotein ⚪: Nuclei 🔬: Joe McKellar #SciArt #MicroscopyMonday

Wild vs. Domesticated 🐯🐱 ​Immortalized cell lines live a cozy life, but these rugged primary mouse ear skin fibroblasts vibe differently: ​🩷: Actin 🩵: DNA ​Look at those actin cables and cells climbing over each other, they haven't forgotten where they came from! 🔬: Joe McKellar

Welcome to The Grid 🕹️⚡ ​Mouse cells playing TRON 8 hours post-infection with VSV. ​🟧 VSV-G: Membranes 🟦 GFP: Cytoplasm ⬜ DAPI: Cores ​The viral program has rewritten the host code. Orange circuits queuing at the perimeter. Next sector loading... 🔬: Joe McKellar #SciArt #MicroscopyMonday

Cellular Wormhole 🌀 The images I've been processing recently have me in a major sci-fi mood! A glowing microscopic wormhole: 🟢: Actin 🟣: Tubulin The microtubule web pulling toward the dark core gives event horizon vibes 👀 Where does it lead? 🔬: Joe McKellar #FluorescenceFriday

Actin in a fibroblast for #MicroscopyMonday. What type of actin structure is your favourite? :) 🔬 : Joe McKellar, Viroscope Imaging

Cellular Uniqueness 🧬✨ ​Clonal cell lines can seem uniform, but under the scope, every cell has its own spatial secrets. 💜 Magenta: Membrane 💛 Yellow: DNA 🩵 Cyan: Microtubules ​Notice the two microtubule exclusion zones? What's your theory? 👇 ​ 🔬: Joe McKellar

End of a Microscopic Galaxy 🌌🔥 This high-density neuronal culture screams cosmic web collapsing into stellar fire. 🔥Fiery Orange: Actin ❄️Cool Blue: DNA Sometimes, looking down through a microscope allows us to look up at the cosmos. 🔬: Joe McKellar

Viral Takeover ☣️ A microscopic siege frozen in time, as cellular life gives way to viral progeny. HEK293T cells infected with VSV: 🔴 Viral glycoprotein 🔵 DNA 🔬: Joe McKellar, @viroscope.bsky.social

Cellular Ballet 💃 Two boa constrictor brain cells reaching through the dark to perform a microscopic ballet. 🟢 Membranes 🟡 DNA ⚪ Actin The dance of life is always a spectacle to behold. 🔬: Joe McKellar

The final part of Ask the Expert! Joe McKellar PhD (@viroscope.bsky.social) closes the series with one tip for every researcher just starting out in IF. Simple. Essential. Thank you, Joe 🔬 Antibodies by Proteintech. Imaged using @zeiss-microscopy.bsky.social LSM880. #Immunofluorescence

Reconstituting mitosis ✨ I recreated a full cell division sequence using 9 separate cells I found from old files! 🟠 DNA ⚪ Tubulin (look the different structures!) 🟣 Actin Which stage is your favorite? 👇 🔬: Joe McKellar

Does the colour scheme you choose for a multiplex IF image matter scientifically? Joe McKellar PhD (@viroscope.bsky.social) breaks it down in Part 7 of Ask the Expert. 🟡 KIF5B (21632-1-AP) 🔵 IAV nucleoprotein 🟣 DNA Imaged on @zeiss-microscopy.bsky.social LSM880 Airyscan. #Immunofluorescence

*cell culture vibes* 🔬: Joe McKellar

How do you design a multiplex IF experiment with zero bleed-through? Joe McKellar PhD (@viroscope.bsky.social) breaks it down in Part 6 of Ask the Expert. 🟡 KIF5B (21632-1-AP) 🔵 IAV nucleoprotein 🟣 DNA Imaged on @zeiss-microscopy.bsky.social LSM880 Airyscan. #AskTheExpert #Immunofluorescence

Cosmic horror at the sub-cellular scale 🌠🐙 Mutant mouse Mx1 (green) forming tentacle-like structures crawling through a genomic portal (pink; DNA). Like Cthulhu breaching the barriers between realms... Years later, these geometries remain unexplained. 🔬: Joe McKellar

Good antibody or great antibody: what's the difference, and can you see it in an image? Joe McKellar, PhD, @viroscope.bsky.social breaks it down in Part 5 of Ask the Expert. 🟡 CYFIP1/2 (16011-1-AP) 🔵 DNA Imaged on @zeiss-microscopy.bsky.social LSM880 Airyscan #AskTheExpert

The hidden geometry of the cytoskeleton 📐✨ Swipe to see the weird and wonderful world of Septin geometries in this giant human cell: 🟠Septin 7 🔵Actin 🟣DNA 🔬Joe McKellar

Check out the latest part of our Ask the Expert video series with @proteintech.bsky.social, where we talk about some common imaging questions ✨🔬

🔵 ⚪ 🔴 A fitting cellular tricolour: blue microtubule highways, white DNA cores, and red membranes. Endlessly grateful to France for the possibilities it has opened up for @viroscope.bsky.social. Bonne fête du 14 Juillet à tous ! #14Juillet #BastilleDay #FranceScience #Microscopy #SciArt

Your experiment "didn't work." But did it? Part 3 of Ask the Expert with @viroscope.bsky.social x Proteintech: negative result vs protocol failure, and how to tell them apart. 🟡 CYFIP1/2 (16011-1-AP) 🔵 DNA Imaged on @zeiss-microscopy.bsky.social LSM880 Airyscan. #AskTheExpert #Immunofluorescence

Painting the cellular cosmos🎨🔬 In science, the boundary between experiment and art can dissolve. Cells become a canvas in total darkness where fluorophores are paint and lasers are our brush. 🟩 DNA 🟧 Actin 🟦 Microtubules 🔬: Joe McKellar

Why does IF signal vary between cells in the same image? Spoiler: it's often not your protocol. Ask the Expert with @_Viroscope, answering the questions every IF researcher has. 🟢 MYC-tag (16286-1-AP): https://bit.ly/4gHF0vT 🟣 IAV nucleoprotein 🟡 DNA #Immunofluorescence

Transport through the microcosmos ✨ In a living cell, a standstill is an alien concept. Molecular machines travel tirelessly through the internal geography. 🟧 Microtubules: Structural hyperloops. 🟪 Vesicles: Cargo pods racing around the grid with incredible precision. 🔬: Joe McKellar

The inner cellular cosmos ✨ Mapping cells to bring an infrastructure that operates in the dark to the light. ​◻️ Nucleus: Quiet gravitational center 🟦 Actin: Radiant structural cables 🟧 Septin: Reinforcing scaffolding 🟪 Membranes: Glowing cytoplasmic network Follow for more SciArt! 🔬: Joe McKellar

10h into active viral pathogenesis. Lung cells infected with VSV, showing vesicle trafficking under siege. 🩷 GFP: Replicating virus 💙 Rab11: Endosome network Clear cytopathic distress: extensive membrane blebbing & abnormal nuclear shapes. 🔬 @viroscope.bsky.social. 1 slice, 1 img/sec.

SARS-CoV-2 replicating inside primary Human Airway Epithelia 🟩 Actin: Highlight brush-like cilia and cell-cell junctions 🟪 dsRNA: Replicating virus This image made the cover of Journal of Virology for April 2021.

Check out the final part in the series of #InTheLabWithProteintech !

#FluorescenceFriday A Maximum Projection of three U2OS cells capturing two different steps of cell division. Sister cells are split but tethered by a midbody remnant. 💛 Actin: Spiky/explosive 💙 DNA: Varing conformations 🖤 Microtubules: Dark depth Separation takes time.

Inner Space: A Cellular Cosmos Two human fibroblasts mimicking cosmic solar bodies. 💛 Actin: Radiating star filaments 🩵 Tubulin: Plasma highways ❤️ DNA: Crimson core We are a universe ourselves. At Viroscope Imaging, we make data breathtaking. Let's elevate your R&D! 🔬 #FluorescenceFriday #SciArt

Septins: the oft forgotten 4th component of the cytoskeleton. This shot of the basal layer reveals SEPT7 (Gold) and SEPT8 (Cyan) in all their structural glory. Check out the blog post and the @proteintech.bsky.social antibody below! ⏬⏬

The art of the waiting game. Rodent deltavirus (RDeV) is a satellite virus. It doesn't kill, but moves in like a quiet tenant, replicating while waiting for a partner in crime, a helper virus, to provide its transport. 🟨 Actin 🟦 Delta Antigen ⬜ DNA 🔬: Imaged by me.

Building a cellular village 🔬🏘️ You've seen a Tasmanian Devil, but not like this! This super-res cluster of fibroblasts shows one cell building a new unit for the community. 💛 Actin 🩷 DNA 🩵 Tubulin The cyan mitotic spindle is ready for the heavy lifting! Fav mitosis stage? ⬇️

#InTheLabWithProteintech! Go check out the blog post behind the image, and how @proteintech.bsky.social antibodies helped me in my work on viral restriction : bit.ly/4242DX8

The high-speed logistics of a lung cancer cell. Watch GFP-Rab25 (Gold) move cargo through the internal recycling pathway of A549 cells. Static maps are great, but time-lapse shows the real traffic. Want your science/tech to be highlighted? DM me at Viroscope!

Sometimes, the data can wait✨ A COS-7 cell reminding us that biology is art. 🩵 Tubulin: Cyan sunburst 💛 Actin: Yellow lace 🩷 DNA Happy #FluorescenceFriday! I turn raw data into cinematic visuals at Viroscope. If you want your science/tech to stand out, reach out! #SciArt

The anatomical landscape of a zebrafish tail 🔬🐟 🔵 Nuclei: Hundreds of individuals in a dense tissular landscape. 🟠 Microtubules: Notice the axonal structural repeats! Want your science/tech to be highlighted? Reach out to me ! #SciArt #Zebrafish

If a cyberpunk metropolis was engineered by biology, it’d look like this. 🔵 Actin: neon structural cables 🟣 MX1: localized defense matrix 🟠 DNA: reactor cores Want your science/tech to look like a movie poster? Reach out to me! 🔬 #SciArt #FluorescenceFriday

Tracking the rapid recycling pathways of a living cell. This time-lapse captures an A549 lung cell expressing GFP-Rab11a. These fast-moving puncta are recycling endosomes, tirelessly shuttling sorted cargo back out to the plasma membrane. Check out the tubulations also! #SciArt #LiveCellImaging

Cellular Liquid Gold 🔬 This time-lapse shows an A549 cell over-expressing Sec61B, outlining the Endoplasmic Reticulum. Imaged at 1 frame per 500ms, it reveals just how violently active our cellular infrastructure really is! What is your favorite organelle to image? 👇 #SciArt #FluorescenceFriday

Mapping active herpesvirus replication inside human neurons 🧠🔬 A max intensity projection of HSV-1 infecting LUHMES cells. 🔵 Actin: The dense structural network. 🔴 HSV-1: Hotspots of active viral replication. Infections are undesirable, but beautiful! #sciart #virology

Kudos to @nadinelaguette.bsky.social team and all co-authors on this nice paper where our lab had a modest contribution! @viroscope.bsky.social 🔥

Thrilled to share that the French journal "Virologie" has chosen my work as the official 2026 cover!🔬 ➡️To see the original black-and-white capture. It shows primary boa constrictor brain cells infected for 1h with VSV. Thank you to @sfvirologie.bsky.social and @sebnisole.bsky.social ! #SciArt

Hepatitis D virus hitches a ride into host cells inside a helper virus such as hep B acting as a Trojan horse 📹 @viroscope.bsky.social et al @karimaj.bsky.social lab @umontpellier.bsky.social in @cellpress.bsky.social ➡️ bpod.org.uk/archive/2026...