Gabriel Rocklin

New preprint! We measured temperature- and pH-induced aggregation for over 18,000 natural and de novo designed protein domains!

🚀 Excited to share our new work: Absolute Stability Predictor! 📊: forms.gle/4ZnXZSnTBvay... Built the MGnify Stability Dataset (1.8M+ measurements) and developed stability prediction models, together with @grocklin.bsky.social @KotaroTsuboyama, @sokrypton.org and teams.

We've actually been working on this very actively and have quite promising results on larger/higher stability proteins using an improved method. Looking for funding to buy some (a lot of) oligos... any pointers? It's within reach...

Another banger

Thread coming later but I think people will like this!! 2 million stability measurements plus a predictive model for deltaG (not ddG)

Accurate protein stability prediction for small domains using mega-scale experiments https://www.biorxiv.org/content/10.64898/2026.05.19.726285v1

If you missed the preprint, now is a good time to read the journal version of @grocklin.bsky.social et al’s fantastic paper on multiplexed HDX measurements Large-scale discovery, analysis and design of protein energy landscapes doi.org/10.1038/s415...

New lab preprint: if ML struggles with extrapolation, let's expand the diversity of training data with gene synth, DNA shuffling, and ML gen.... also lots of #FluorescentProteins #ProteinEngineering #MachineLearning #SyntheticBiology 1/n

High Diversity Gene Libraries Facilitate Machine Learning Guided Exploration of Fluorescent Protein Sequence Space https://www.biorxiv.org/content/10.64898/2026.03.01.706892v1

I’m looking to hire a research technician for my lab at Harvard & DFCI, who would primarily work in the wet lab expressing and characterizing designed proteins, starting this summer. A great role for a recent college grad looking for an immersive research experience before grad school.

Here’s a preprint from the Keating Lab: www.biorxiv.org/content/10.6.... Foster Birnbaum and Amy E. Keating demonstrate that sequence design models, such as ProteinMPNN, are limited because they were trained only on native sequences.

The final Calvin and Hobbes, which appeared in papers 30 years ago today.

Large scale prospective evaluation of co-folding across 557 Mac1-ligand complexes and three virtual screens [new] Mac1 co-folding eval: Pose prediction, conform. change, & hit ID post-training.

The most precious commodity you have is your attention. You don’t have to waste it on poor-faith debates or arguments with strangers if you don’t think they’ll be productive. You can prioritize the things that matter to you and make your life richer.

Excited to share our newest manuscript on antigen discovery and vaccine design for tuberculosis lead by Owen Leddy (everyone hiring new faculty in a few years, remember this name!) www.science.org/doi/10.1126/... #TBsky

Global Analysis of Aggregation Determinants in Small Protein Domains https://www.biorxiv.org/content/10.1101/2025.11.11.687847v1

quick reminder to come see @grocklin.bsky.social tonight at @mit.edu!

Join us in Room 181, Building 68 @mit.edu on October 22nd 2025 @ 7pm EDT to see @grocklin.bsky.social present a mind blowing amount of data and learn what becomes possible at this scale "Predicting protein folding stability and aggregation propensity using large-scale experiments" bpdmc.org

Announcing such a major change to NSF GRFP this late in the cycle is incredibly cruel. 2nd yr students (mine included) have been working so hard on their proposals despite ongoing uncertainty. They are driven and passionate about being outstanding scientists and helping those coming up behind them.

Cecilia Payne-Gaposchkin ✨ figured out what stars are made of ✨ when she was just 25. 🔭🧪 Her PhD thesis basically established the Harvard astro department — at a time when Harvard didn't officially allow woman students. I wrote this little profile to mark the 100th anniversary of her thesis:

Vought and his “no more NIH money out the door” footnote is a power struggle. Between R’s getting antsy about hard votes and Senate signaling support for science funding, Vought had to signal his dominance in some way. Now is the real test: are R’s more afraid of Trump et al. or their constituents?

The NIH can’t award ANY grants to outside researchers under new WH restriction, reports @wsj.com. The pause came in the form of a footnote from OMB Director Vought, in a document that doles out federal funds to the NIH. Prelude to rescissions, especially after his comments over the weekend?

PO just confirmed that our 5th percentile R01 is not getting funded. Past NCI Payline at 4 percent. Cant believe it.

NCI's payline will be the 4th percentile. The Trump administration has effectively killed Cancer Research in the US www.cancer.gov/grants-train...

A motivating quote from @carolynbertozzi.bskyverified.social: “…You are discovering knowledge and gifting that to humanity for all of posterity. Once you learn something no one can take that away. It is not an object. It is an intellectual currency that will be shared throughout the generations…”

#MorganLabatND is hiring a research technician!! We are looking for an aspiring biochemistry researcher that shares our community values, is curious about the world around them, and brings a unique perspective to our team. (1/2) jobs.smartrecruiters.com/UniversityOf...