International Society for Protein Termini (ISPT)

International Society for Protein Termini (ISPT)

@ispt-proteinterm.bsky.social

Non-profit society of scientists devoted to understanding the molecular mechanisms and biological role of protein termini in plants, animals, fungi and bacteria. Conference every second year. Website: https://proteintermini.org/

196 followers229 following26 posts

International Society for Protein Termini (ISPT) reposted

Isa Manrique
@isamanrique.bsky.social

Our paper is out! 🤩 HYT1, a new Cys2- substrate of the #Ndegron pathway to add to the list! 🌱 Thanks to @n-end-rules.bsky.social @charlene-kunaka.bsky.social @gunjansharma88.bsky.social , Susana and all the people involved in this work! You can read it on @natcomms.nature.com👇🏼

nature.comHierarchical regulation of oxygen-sensing through coupled transducers in Arabidopsis thaliana - Nature CommunicationsThe PLANT CYSTEINE OXIDASE (PCO) N-degron pathway contributes to oxygen sensing in plants. Here the authors identify HYPOXIA TRANSDUCER1 (HYT1) as a substrate of PCO and show it forms a coherent feed-...
N End Rules@n-end-rules.bsky.social

We introduce a new transducer of oxygen sensing: Cys2-HYT1 @isamanrique.bsky.social @charlene-kunaka.bsky.social Susanna Ubeda Tomas & @gunjansharma88.bsky.social ma88.bsky.social lead work defining HYT1 contribution to root hypoxia tolerance. @natcomms.nature.com www.nature.com/articles/s41...

nature.comHierarchical regulation of oxygen-sensing through coupled transducers in Arabidopsis thaliana - Nature CommunicationsThe PLANT CYSTEINE OXIDASE (PCO) N-degron pathway contributes to oxygen sensing in plants. Here the authors identify HYPOXIA TRANSDUCER1 (HYT1) as a substrate of PCO and show it forms a coherent feed-...

International Society for Protein Termini (ISPT) reposted

Molecular Cell
@cp-molcell.bsky.social

Online Now: Proteome-wide C-degron activity profiling connects conditional regulation of the CTLH E3 ligase complex to ribosome biogenesis Online now:

dlvr.itProteome-wide C-degron activity profiling connects conditional regulation of the CTLH E3 ligase complex to ribosome biogenesisExploiting ORFeome libraries with different C-terminal architectures, Grant et al. perform comparative stability profiling to identify a conditionally regulated C-degron pathway governed by the CTLHMuskelin E3 ligase complex. Its substrate AAMP is the human ortholog of yeast Sqt1 and acts similarly as a uL16 chaperone, connecting nutrient status to control of ribosome biogenesis.

International Society for Protein Termini (ISPT) reposted

Mikel Lavilla-Puerta
@mikellavi.bsky.social

Very happy to see this out!! Thank you all! Check out or latest publication www.pnas.org/doi/10.1073/...

pnas.orgA synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants | PNASPlants face hypoxic conditions either chronically, as particular tissues are characterized by fluctuating or stable low oxygen levels, or acutely, ...
Oxford Biology@biology.ox.ac.uk

Without oxygen, the first minutes matter 🌱 In low oxygen, eg when flooded, plants need to act quickly to survive. New research led by @mikellavi.bsky.social has revealed a molecular circuit that kicks into gear in minutes - and it can work completely independently 👇

bit.lyWithout oxygen, the first minutes matter: a molecular circuit helps plants survive

International Society for Protein Termini (ISPT) reposted

Thomas Arnesen
@natmachinery.bsky.social

Impaired protein N-terminal acetylation may reduce sensitivity to skin pain, strongly spiced food, being tickled, and cold weather | new case report by Bjørnar Hassel and Nina McTiernan et al. @ispt-proteinterm.bsky.social @bmc.springernature.com link.springer.com/article/10.1...

link.springer.comInsensitivity to skin pain, tickling, and spicy food in a woman with a pathogenic NAA10 variant: a case report - BMC Medical GenomicsBackground The NAA10 gene on the X chromosome encodes NAA10, the catalytic subunit of the N-terminal acetyltransferase complex NatA, which co-translationally acetylates nearly half the human proteome....

International Society for Protein Termini (ISPT) reposted

Thomas Arnesen
@natmachinery.bsky.social

Coenzyme A biosynthesis is regulated by two distinct Ubiquitin E3 ligase complexes | UBR4-KCMF1 and MKLN1-CTLH independently converge on a common N-terminal MK Arg/N-degron to promote PANK degradation and restrict CoA biosynthesis | @ispt-proteinterm.bsky.social www.biorxiv.org/content/10.6...

biorxiv.orgParallel Arg/N-degron recognition systems regulate coenzyme A biosynthesisCoenzyme A (CoA) is an essential metabolic cofactor whose biosynthesis is controlled by pantothenate kinases (PANKs), the rate-limiting enzymes in CoA synthesis. Although CoA production is extensively...

International Society for Protein Termini (ISPT) reposted

N End Rules
@n-end-rules.bsky.social

We introduce a new transducer of oxygen sensing: Cys2-HYT1 @isamanrique.bsky.social @charlene-kunaka.bsky.social Susanna Ubeda Tomas & @gunjansharma88.bsky.social ma88.bsky.social lead work defining HYT1 contribution to root hypoxia tolerance. @natcomms.nature.com www.nature.com/articles/s41...

nature.comHierarchical regulation of oxygen-sensing through coupled transducers in Arabidopsis thaliana - Nature CommunicationsThe PLANT CYSTEINE OXIDASE (PCO) N-degron pathway contributes to oxygen sensing in plants. Here the authors identify HYPOXIA TRANSDUCER1 (HYT1) as a substrate of PCO and show it forms a coherent feed-...

International Society for Protein Termini (ISPT) reposted

Frederica Theodoulou
@freddietheodoulou.bsky.social

Ever wondered why mutating BIG/AtUBR4 causes pleiotropic 🌿 phenotypes? Find a whistle stop tour of the literature in our latest review, with expert structural insight from @dangrabarczyk.bsky.social and @clausenlab.bsky.social academic.oup.com/jxb/advance-...

academic.oup.comDecoding BIG: how one protein influences multiple pathwaysAbstract. A major strength of forward genetic screens is their unbiased nature: the plant reveals which genes underlie a physiological process. Yet this st

International Society for Protein Termini (ISPT) reposted

Thomas Arnesen
@natmachinery.bsky.social

Symbiosis: An N-terminally acetylated peptide from the bacterium Photorhabdus modulates nematode development | Novel prokaryotic NAT enzyme PasC | @ispt-proteinterm.bsky.social www.pnas.org/doi/10.1073/...

pnas.orgAn N-acetylated daropeptide modulates nematode development | PNASThe symbiotic bacterium Photorhabdus is a rich source of bioactive secondary metabolites that mediate tripartite interactions with nematodes and in...

International Society for Protein Termini (ISPT) reposted

Laurenz Rabl
@laurenzrabl.bsky.social

More on NAC and cotranslational Nt-acetylation by NatB. Congratulations to the first authors Natalia and Pawel! rdcu.be/ftSay

rdcu.beStructural basis of cotranslational protein N-terminal acetylation by NatB in human cellsNature Communications - N-terminal acetylation is one of the most common protein modifications. Here, the authors show how NAC recruits the essential enzyme NatB to translating ribosomes, revealing...

International Society for Protein Termini (ISPT) reposted

Thomas Arnesen
@natmachinery.bsky.social

α-Synuclein N-terminal acetylation tunes both its structural and physicochemical properties to enhance binding to lipid bilayers @ispt-proteinterm.bsky.social pubs.acs.org/bichaw/artic...

pubs.acs.orgN-Terminal Amine Chemistry Influences α-Synuclein Interactions with Lipid BilayersAbstract. α-Synuclein (αSyn) is an intrinsically disordered protein whose reversible membrane binding via amphipathic α-helix formation is central to its f

International Society for Protein Termini (ISPT) reposted

Jorge Vicente
@yorgsvicen.bsky.social

Our new preprint shows that metacaspase-mediated proteoform generation and N-degron pathway-dependent proteoform stability act together to diversify UBP6 function during Arabidopsis immunity www.biorxiv.org/content/10.6...

biorxiv.orgFunctional diversification of UBP6 in plant immunity through N‑degron pathway regulationDeubiquitylases are key proteolytic regulators of ubiquitin-dependent cellular processes, catalyzing the removal or remodelling of ubiquitin modifications on substrate proteins, including those target...

International Society for Protein Termini (ISPT) reposted

Frederica Theodoulou
@freddietheodoulou.bsky.social

A conference about #proteostasis in a palazzo in Palermo; inclusive, affordable, and organised by the marvellous @giglionelab.bsky.social - what’s not to like? Oh, and there is a fantastic lineup of talks plus slots to spare #ProteinTermini2026

International Society for Protein Termini (ISPT)@ispt-proteinterm.bsky.social

See you at Palazzo dei Normanni in Palermo for the 2026 Protein Termini Conference | Co- and post-translational modifications | Proteolytic processing and degradation pathways | Microbes, plants, animals #ProteinTermini2026 #Proteostasis #ProteinModifications #PalazzoDeiNormanni #Palermo2026

International Society for Protein Termini (ISPT) reposted

giglionelab.bsky.social
@giglionelab.bsky.social

The countdown to Palermo is on! 🇮🇹 The FEBS Workshop "Protein Termini 2026" is set. • 151 participants from all over the world. • >50% ECRs! • 30+ travel grants. Thx to sponsors: FEBS, IUBMB, CDBC, ARS, and others.. See you at Palazzo dei Normanni! #ProteinTermini #FEBS #Proteostasis

International Society for Protein Termini (ISPT) reposted

Thomas Arnesen
@natmachinery.bsky.social

New review out on protein N-terminal formylation - a stress-inducible modification of eukaryotic proteins #N-degron @ispt-proteinterm.bsky.social www.nature.com/articles/s12...

nature.comN-terminal formylmethionine as a degron and a specific signal in proteostasis and stress adaptation - Experimental & Molecular MedicineN-terminal methionine formylation, a protein modification once thought to be exclusive to bacteria and organelles, also occurs on proteins synthesized in the cytosol of yeast and human cells, challeng...

International Society for Protein Termini (ISPT) reposted

dave dougan
@douganlab.bsky.social

A C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation www.pnas.org/doi/10.1073/...

pnas.orgA C-degron regulates Chk1 kinase by allowing stability of inactive Chk1 and by making it short- lived upon activation | PNASThe Arg/N-degron pathway of Saccharomyces cerevisiae is mediated by two interacting E3 ubiquitin ligases, Ubr1 and Ufd4. We show here that the mito...
International Society for Protein Termini (ISPT)
@ispt-proteinterm.bsky.social

Resistance to prolonged darkness in plants steered by N-terminal acetylation | New study by Gong et al. Markus Wirtz lab @giglionelab.bsky.social Tanja Bange lab #NatB #autophagy #N-degron #proteostasis www.nature.com/articles/s41...

nature.comThe ribosome-associated N-terminal acetyltransferase B coordinates global proteostasis and autophagy in plants by creating Ac/N-degrons - Nature CommunicationsProtein N-terminal acetylation profoundly influences protein fate. Here, the authors establish NatB as a central regulator of the interplay between ubiquitin–proteasome system and autophagy, and highl...

International Society for Protein Termini (ISPT) reposted

Beckmann Lab
@beckmannlab.bsky.social

How are histones acetylated as they emerge from the ribosome? We show that NAA40 cooperates with the NAC complex to enable co-translational acetylation of H2A/H4, revealing NAC as a coordinator of nascent protein modification. Read more about our collaboration with @kirmizislab.bsky.social #cryoEM

rdcu.beNAA40 and NAC cooperate in co-translational histone acetylation in humansNature Communications - N-terminal histone acetylation by human NAA40 alters epigenetic signaling and affects gene regulation. Here, the authors combine biochemistry and cryo-EM to unravel the...

International Society for Protein Termini (ISPT) reposted

Mikel Lavilla-Puerta
@mikellavi.bsky.social

Finally officially out!! www.pnas.org/doi/10.1073/... We moved the main oxygen sensing machinery from plants to yeast and we learned a few things!

pnas.orgA synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants | PNASPlants face hypoxic conditions either chronically, as particular tissues are characterized by fluctuating or stable low oxygen levels, or acutely, ...

International Society for Protein Termini (ISPT) reposted

Dan Gibbs 🌱🧬
@djgibbs.bsky.social

My review “Co-translational control of protein stability and quality in plants” is now online at @jxbotany.bsky.social, in which I describe how co-translational processing and ribosome-associated quality control together establish protein stability and fate early in synthesis. tinyurl.com/5dhhz9h6

tinyurl.comCo-translational control of protein stability and quality in plantsAbstract. Proteostasis relies on the coordinated control of protein synthesis, folding, modification and degradation, and an increasingly clear picture is

International Society for Protein Termini (ISPT) reposted

David Balchin
@davidbalchin.bsky.social

Join us at the @crick.ac.uk for the 2026 meeting of the UK proteostasis community! We especially encourage students and postdocs to attend and share their work. All talks (except the keynotes) will be selected from abstracts.

Proteostasis UK@proteostasisuk.bsky.social

📣 UK Proteostasis Meeting 2026 – Registration Now Open! I’m delighted to share that registration is now open for the UK Proteostasis Meeting 2026, hosted by The Francis Crick Institute on 20–21 July 2026
. Please register here(lnkd.in/ervXMzWN) and through Eventbrite for payment (lnkd.in/eTxqjnQy)

International Society for Protein Termini (ISPT)
@ispt-proteinterm.bsky.social

Protein degradation in Turnip - an adapted Arg/N-degron pathway. Preprint from Brian Mooney et al. Emmanuelle Graciet lab. www.biorxiv.org/content/10.6...

biorxiv.orgFunctional divergence of the Arg/N-degron pathway between the crop Brassica rapa and the model plant Arabidopsis thalianaThe ubiquitin-dependent Arg/N-degron pathway relates the stability of a substrate protein to the nature of its N-terminal amino acid residue or its biochemical modifications, with some N-terminal residues being recognized by specific E3 ubiquitin ligases, resulting in the ubiquitylation and degradation of the substrate protein. Work in the model plant Arabidopsis thaliana has shown that the Arg/N-degron pathway is a key regulator of plant responses to hypoxia, which can be either physiological or a stress in the context of waterlogging or submergence. The role of the Arg/N-degron pathway in hypoxia response is mediated via the oxygen-dependent degradation of group VII ETHYLENE RESPONSE FACTOR (ERFVII) transcription factors, which act as the master regulators of the hypoxia response program in plants. Analysis of Arabidopsis mutants for different enzymatic components of the Arg/N-degron pathway has also revealed its roles in the regulation of responses to other abiotic stresses (e.g. salt stress), as well as to pathogens. Although much has been learned from studies in Arabidopsis about the functions of the Arg/N-degron pathway, very little is known about this pathway in crops, including in Brassica crops such as oilseed rape, cabbage or turnip. To determine functional similarities and divergence of the Arg/N-degron pathway between Arabidopsis and Brassica crops, we isolated and characterized the first Arg/N-degron pathway mutants in Brassica rapa (turnip, pak choi), a diploid Brassica crop closely related to oilseed rape. We focused on two enzymatic components, namely the arginine-transferases ( ATE s) and the E3 ubiquitin ligase PROTEOLYSIS6 ( PRT6 ). Our results show both similarities and divergence of function for these Arg/N-degron pathway components in B. rapa compared to Arabidopsis. Specifically, ATE mutants in B. rapa arrest their development at the seedling stage, which contrasts with the mild phenotypic defects of the equivalent Arabidopsis mutants. Double mutant lines for two of the three PRT6 genes in B. rapa indicated a constitutive activation of hypoxia response genes at the transcriptional level, as shown in the single prt6 mutant in Arabidopsis. However, contrary to Arabidopsis, the B. rapa double mutants were more sensitive to waterlogging and hypoxia, and did not show differential response to salt stress or to biotic stress compared to the wild type. The functional divergence identified likely reflects variability in each species in the substrate repertoire and/or in the regulation of pathways or targets downstream of Arg/N-degron pathway substrates. Such differences could be driven by direct selective pressures at N-termini (e.g. gain or loss of a destabilizing N-terminal residue), or by species-specific proteases that may generate destabilizing neo-N-termini after cleavage. These similarities and differences highlight the difficulties in translating research findings from Arabidopsis to crops, even within the same plant family (Brassicaceae) and highlight the need to study pathways in crops. ### Competing Interest Statement The authors have declared no competing interest. Science Foundation Ireland, https://ror.org/0271asj38, 13/IA/1870, 20/FFP-P/8433 Irish Research Council, https://ror.org/051xex213, GOIPG/2017/2
International Society for Protein Termini (ISPT)
@ispt-proteinterm.bsky.social

Ribosome-NAC collaboration: A regulatory platform for cotranslational chaperones, enzymes, and targeting factors. New review out in @cp-molcell.bsky.social from Elke Deuerling's lab. www.sciencedirect.com/science/arti...

sciencedirect.comRibosome-NAC collaboration: A regulatory platform for cotranslational chaperones, enzymes, and targeting factorsProtein biogenesis requires the ribosome to collaborate with a diverse set of cotranslational factors that shape the fate of nascent chains. These int…

International Society for Protein Termini (ISPT) reposted

Thomas Arnesen
@natmachinery.bsky.social

Complexity at the ribosomal polypeptide tunnel exit. The major N-terminal acetyltransferase NatA may form different complexes on the ribosome to facilitate protein maturation. By Marius Klein & Klemens Wild in Irmgard Sinning's lab BZH & Nina McTiernan in my lab. www.nature.com/articles/s41...