Felipe Opazo

Just published 🎉🥳 Two-photon #FLIM of NAD(P)H is a promising readout for cellular metabolic changes. However, multiplexing with fluorescent proteins is surprisingly hard🤔😅 ... But it can be navigated! www.cell.com/biophysrepor... @biophysreports.bsky.social

Neue Einblicke in die kleinsten Strukturen des Lebens: Forschende unter Leitung unserer Uni und der UMG konnten mit sogenannten selbstblinkenden Farbstoffen #Zellstrukturen präziser und zuverlässiger abbilden, als es mit konventionellen Farbstoffen möglich ist: s.gwdg.de/veokJA; doi.org/rrwk

Registration is finally OPEN for the 5th Single Domain Antibodies Conference 2027! 🦙 📍 Göttingen, Germany · 14–16 June 2027 Nanobody / VHH / sdAb aficionados, come join us. Strong speaker lineup, and still growing. Please share the news! 👉 single-domain-antibodies.org #Nanobodies #VHH #sdAb2027

📄This new Journal of Microscopy 🔬 paper uses rigorous single-molecule assays to benchmark secondary nanobodies and shows NanoTag #SmartSecondaries® deliver high-affinity binding, efficient saturation, and exceptionally low off-target labeling for robust #multiplexed immunoassays 💪

Never worked with #SmartSecondaries® (secondary nanobodies) for detecting conventional or recombinant primary antibodies? Here you can find more information: nano-tag.com/technology/s...

Spontaneous blinking. OneStep IF. Precise #SMLM. 🙌 Our Smart Secondaries® JF635b combine a self-blinking dye with small secondary #nanobodies for reduced linkage error, no clustering & fast OneStep IF. For #dSTORM & #MINFLUX 🔬✨ 🦙 Browse our JF635b-conjugated nanobodies here: bit.ly/4ffIkdB

Are you looking for a postdoc exploring the cell biology of development, defining how cells change shape and move? Do you want to develop a research program & teaching skills preparing you for a position at a college or small University? Our new NIH grant will support those goals 1/n Please share

✨🔬 Want to see our JF635b-conjugated #nanobodies in action? 📄 Check out the recent NanoLetters paper by Samrat Basak @samratb.bsky.social, Roman Tsukanov @romantsukanov.bsky.social, Felipe Opazo @opazo.bsky.social et al., doi.org/10.1021/acs....

Here's a balanced account of where the microscopy community stands on AI use by this year's Small World in Motion winner. My main issue is that no one seems to have seen the original data despite requests (no mention of it being reviewed by the organizers) www.the-scientist.com/allegations-...

Bei einer neuen Markierungsmethode werden fluoreszierenden Marker nicht anhand ihrer Farbe, sondern ihrer Leuchtdauer unterschieden. Dadurch lassen sich mit Standardmikroskopen acht verschiedene Proteine in einer Zelle gleichzeitig sichtbar machen. @opazo.bsky.social pubs.acs.org/ancac3/artic...

🚨 Our new @elife.bsky.social study dissects how a dividing cell keeps its septin scaffold and its contractile ring in step. 👉 elifesciences.org/articles/106... Spearheaded by @bindubhojappa.bsky.social #cellbiology #celldivision #septins #yeast #kinases

#NanoTag mindset: 𝗡𝗼 𝗦𝗹𝗼𝗴𝗮𝗻𝘀. 𝗝𝘂𝘀𝘁 𝗦𝗰𝗶𝗲𝗻𝗰𝗲. We started in academia, developing #nanobodies for our own research - and we still approach our reagents as scientists: develop in-house, test thoroughly, characterise and share the data 📄. Because only reliable tools move #science forward 🚀

New publication alert 🚨 Our Nano Letters paper on self-blinking dyes with nanobodies for robust SMLM is now Open Access! pubs.acs.org/nalefd/artic... Commercial Nb–JF635b probes are available from @nanotag.bsky.social. Joint effort of amazing collaborators, inspired by the great @opazo.bsky.social

We love using mNeonGreen for live but have failed to find good reagents for things like expansion microscopy. This is a fantastic addition to the toolbox. Great work!

Proud of our team and grateful to everyone who contributed to this journey. This paper reflects our shared passion for science and our commitment to developing reliable research tools for the scientific community. 🦙💚🔬

A High-Affinity Nanobody Recognizing mNeonGreen Enables Versatile Biochemical, Cellular, and in vivo Applications. by Steffen Frey and team: www.biorxiv.org/content/10.6...

8 hours. That’s all fish need to heal wounds 🐟⏱️ Why are they so fast compared to humans? I break down the science of rapid regeneration in a microscopy story 🔬 Watch here: youtu.be/Mt9f61adaP0 #FluorescenceFriday #Microscopy

Our new paper in @plosbiology.org introduces IntAct-U-ExM enabling isoform-specific imaging of actin at nanoscale across yeast, mammalian cells & primary neurons. Fabulous work spearheaded by @anubhavdhar.bsky.social along with an amazing team! #actin #U-ExM journals.plos.org/plosbiology/...

Make HDAC6 visible again! 6SiR-C3-NextA is a selective far-red probe for imaging endogenous HDAC6 in living cells across diverse mammalian cell types. Done by @vanthang.bsky.social at @mpi-nat.bsky.social Now in JACS @pubs.acs.org pubs.acs.org/doi/10.1021/...

Resolution isn't just optics. Structural resolvability also depends on probe size, linkage error, labelling efficiency, steric hindrance and, molecular organisation. VLab4Mic models all of these starting from PDB or AlphaFold structures

It is finally out there in published form. 🎉 #MicroSplit (with an 𝕊) #Microscopy 🔬 #AI 🤖 www.nature.com/articles/s41... And no paywall... so... enjoy the read! 💸 👋 This is where the post could end... but I will have to go on for a bit below... 😉 🧵

New paper out! Left-handed DNA cannot bind to natural, right-handed DNA. This leads to lower background if it is used as a probe in DNA-PAINT. Using Fluorigenic DNA-PAINT with left-handed DNA allows for low background, fast 3D DNA-PAINT! Congrats, Bas! skeetorial: 1/x pubs.acs.org/doi/full/10....

There is still time to apply. I'm now looking for someone who likes imaging, for a project like this: www.cell.com/neuron/fullt...

📣 Neuroscientists – something NEW for you! NanoTag expands its portfolio of neurobiological #rHcAbs 🧠. Nanobodies targeting #SYT1 and #CNPase are now available as Mouse or Rabbit IgG Fc fusions – ideal for ICC and IHC 🔬. Learn more 👉 bit.ly/4cKITOd #Neuroscience #RecombinantAntibody

🚀 Just published! In PhD work by first author Dongchen Du, we’ve developed a simple, byproduct-free labeling strategy using imidazopyridinium dyes formed in situ from isoquinolines! onlinelibrary.wiley.com/doi/full/10.... #Fluorescence #Bioconjugation #ChemicalBiology #InSituLabeling

Delighted to share our latest preprint "Filopodia-mediated trans-endocytosis" www.biorxiv.org/content/10.6... Work driven by many people including Hanna, Marcela, Sujan, Marie-Catherine, Anna and Monika. Fantastic collaboration with Johanna Englund, Emilia Peuhu, and Eija Jokitalo

🧪🧬 New preprint! What if the same biosensor could reveal how cysteine is regulated across life domains? We introduce Cystector, a genetically encoded fluorescent biosensor for real-time cysteine monitoring in living cells. www.biorxiv.org/content/10.6... 🧵A thread ⬇️

Last project of the year: self blinking JF635 + lattice light sheet + #BigVolumeBrowser to deskew both volume and localization data (and render) @zeiss-microscopy.bsky.social

We also tried it recently and indeed, it blinks nicely without any smelly buffers or extra stuff added. Makes SMLM sample preparation a breeze. I hope soon new additional colors will be discovered, apart from the far red.

🎄 A glimpse into #NanoTag Christmas dinner - Glühwein outdoors, a shared dinner, and Schrottwichteln 🎁♻️ where funny, used items are exchanged and find a new home. The past year has been special for us as we celebrate 10 years. Grateful for our team, customers, and partners. Here’s to what’s next 🥂

Happy to tell that I got my tenured professorship at the University Medical Center in Göttingen 🇩🇪! Grateful for my students, mentors, international collaborators, the @nanotag.bsky.social team, and my family (dog included 🐾). Motivated to keep pushing science, innovation, startups, and education 🌍✨

#NanoTag joins the Life Science Ecosystem at Nordic Life Science Days #NLSDays2025 in Gothenburg!🇸🇪 Meet our CEO Hansjörg Götzke at the Booth B03:46 (German Pavilion), and discover our sdAb platforms🦙, protein production services, and research tools🔬advancing #LifeScience & #Biomedicine.

Fantastic talk of @ewerslab.bsky.social with ExM data on Septins and Unroof Cells in #GEF25!

🦙 NanoTag at #SingleDomainAntibodies Conference 2025! Our CEO Hansjörg Götzke will be in Utrecht sharing how our immune & synthetic sdAb platforms drive advances in research, diagnostics & therapeutics. Our founder Felipe Opazo @opazo.bsky.social will also be there to connect & share insights.

#ExM turned 10 this year - just like #NanoTag! 🎉 Join us & the super-resolution community in Göttingen for the ExM Forum! 🔬Hear from our founder Felipe Opazo @opazo.bsky.social and CSO Steffen Frey on how NanoTag’s sdAb solutions empower nanoscopy & #multiplex imaging. See you in Göttingen! #GEF25

Prefusion gB of HSV bound with a neutralizing nanobody www.nature.com/articles/s41...

🔬Wrapping up #SMLMS2025 @smlm-symposium.bsky.social in Bonn! Thanks to everyone who stopped by our booth! It was great connecting and sharing how our #sdAb-based solutions enhance precision, simplify #Multiplexing, and streamline workflows in #SuperResolution microscopy. See you next year! 🦙

Out today in @nature.com: Together with the Honigmann, Shevchenko, Drobot and Hof labs, we present a general workflow for imaging the localization and transport of individual lipids in cells and mapping their metabolism. www.nature.com/articles/s41...