Sasha | Kai Dao 🔬

8 hours. That’s all fish need to heal wounds 🐟⏱️ Why are they so fast compared to humans? I break down the science of rapid regeneration in a microscopy story 🔬 Watch here: youtu.be/Mt9f61adaP0 #FluorescenceFriday #Microscopy

Regarding the Nikon Small World controversy, I made a short explaining our concerns to the public—and why data integrity in microscopy matters: youtube.com/shorts/t1hlH... And just now seeing the LinkedIn breakdown showing Gemini generative AI altered the entry makes the whole outcome even sadder.

🔬 New publication, now out in #ScienceImmunology led by Mathieu Deygas in Pablo Vargas team, shows that Adaptive motility enables neutrophils to rapidly navigate confined capillaries 📰 To read the article: tinyurl.com/52vxuhmp

Time-lapse video of vascular development in a transgenic #zebrafish embryo. Credit to @kuglerelisabeth.bsky.social. #ZebrafishZunday

Light sheet fluorescence microscopy (LSFM) movie of axon branching during #zebrafish development in a neurodegenerative disease gene (KLC4) mutant. Credit to @drlizhaynes.bsky.social. #ZebrafishZunday

Happy #FluorescenceFriday. Its always exciting when i get to test out new constructs in my cells. It's not common that I get to see the ER network in an immune cell. This construct is not strictly ER but it's so overly expressed that it labels the ER in an embryonic macrophage in 🐸. How cool?

#fluoroscencefriday It is all about blood vessels and plaques by the @mind-western.bsky.social

This afternoon's imaging lab at #IALSJanelia is all about subcellular structures, highlighted here by myosin (white) and actin (orange). These membrane ruffles were captured on our lattice light-sheet microscope by @cmhobson.bsky.social

Excitable RhoA dynamics drive pulsed contractions in the early #Celegans embryo. From Jonathan B. Michaux, François B. Robin, William M. McFadden, and Edwin M. Munro: rupress.org/jcb/article/... 📕 Part of "Collective Behaviors and Self-Organization in Cells" rupress.org/jcb/collecti...

Do #tintinnids dream of electric sheep? 🤯 Here is a single cell capable of building a lorica and living inside it for protection... it also swims, feeds, hunts Can even detect and habituate to mechanical cues! #celllearning #protistsonsky #cilia Read more in our preprint doi.org/10.64898/202...

I'm happy to share a recent study by @cathygalbraith.bsky.social & James Galbraith, along with @sciencethecat.bsky.social & myself. It challenges existing views on intracellular transportation… 🔗 www.nature.com/articles/s41...

An intact human heart, rendered transparent. Because disease doesn't happen in 2D. Whole-organ tissue clearing and light-sheet microscopy reveal coronary vessels (magenta) and atherosclerotic plaques (gold) across the intact organ. More at: shorturl.at/iqTBh 🫀🔬 #FluorescenceFriday!

Two cells videoed through a microscope. One has big membrane blebs (right) and one has little membrane blebs (left). What do they have in common. They will be dead soon...... #CellBiology #Apoptosis

(3/n) We engineering a synthetic 339 kb E-P pair in 'vacant' region - Vary E-P dist - +/- CTCF at E and P - See nice dot in RCMC maps - Can track E-P 3D distance and nascent RNA from 2Hz to 6+ hours using Super-Res Live Cell Imaging - Txn strictly dependent on E. - New DNA fluor label: synBsr1 (!)

I had fun editing these videos 😎 Whole-body imaging is really opening new possibilities for biology. Seeing the whole picture changes the questions we can ask, and ultimately the discoveries we can make. Read more: 🔬 DISCO-seq www.biorxiv.org/content/10.6... 🚀 LipiGo www.biorxiv.org/content/10.1...

Happy #FluorescenceFriday! Eye of a zebrafish embryo🐟

Rhythmic beating of a transgenic zebrafish embryo’s heart. Credit to @helenevignes.bsky.social, @cvagenapantoula.bsky.social & @vermotlab.bsky.social. #ZebrafishZunday 🧪

From single-celled organisms to ciliated epithelia in metazoans, precise positioning of basal bodies (BBs), which anchor motile cilia, is essential for generating fluid flows. Here, I, @raghavan-thiaga.bsky.social, will take you on a brief tour of how cells build this spatial organization.

What if you got to work with some of your best friends on a science project? I can't publish how fun this was, but I can show you the data (🧵)! Last week, we posted our second neutrophil swarming paper to bioRxiv and I wanted to post my favorite videos here~ www.biorxiv.org/content/10.6...

Not many people know this but 🐸 embryos make an amazing system to study immune cells. If 🐟 embryos become less transparent as they grow, 🐸 embryos do the reverse, becoming progressively transparent. Their immune cells are very similar to 🐟 and 🚶‍♂️. Here are some 🐸 macrophages (🔴): #FluorescenceFriday

Far from a static structure, the ER actively shapes its environment. See how its motility allows it to physically shape the cellular environment in this microscopy footage from Dong Li (CAS) & Andy Moore (Janelia): youtu.be/DnOYwPLhq60 doi.org/10.1016/j.ce... #FluorescenceFriday #Microscopy

Annual killifish continue to surprise us: they make neutrophils before almost every other cell type, even before gastrulation. Neutrophils usually arise from the lateral plate mesoderm (forming in cyan), but in killifish they are already patrolling around the embryo in gold. tinyurl.com/killiphil

Sub-cellular architectures arise through integrating signaling and structure. @edenchang.bsky.social and @zjmaggiexu.bsky.social show how coupling reaction-diffusion signaling to protein condensation provides a tunable, regulatable landscape for sub-cellular structure www.biorxiv.org/content/10.6...

Working with complex systems makes even the most mundane thing fascinating. This is cortical reaction in 🐸 embryos. Upon fertilisation, ions are released and draw water in thru osmosis, making zygotes float and orient according to gravity. Do you see the waves?👀 not quite #fluorescencefriday #devbio

Fruit fly embryo development is wild! 1 nucleus clones into 6,000+ copies in just 2 hours. Perfectly synchronized. With built-in bumper system. And absolutely mesmerizing. I visualized the science behind it in a microscopy story 🔬👇 youtu.be/xyV9BhIVRjM #FluorescenceFriday #Microscopy #Drosophila

🤔How much energy do cellular processes cost, and how does this affect self-organization of living systems? 🔬The Yang group at PoL develops methods to map energy fluxes in cells with subcellular resolution, uncovering the energetic costs/constraints that shape life YouTube video👉 y2u.be/HDA6_58t0Jw

This is why we love #CalciumSignaling Look how mechanical damage triggers long range Ca2+ waves in this plant !! By @annalisabellandi.bsky.social, who is now around here ;) Full www.science.org/doi/10.1126/... @science.org #microscopy #cell #mechanobiology 🧪🔬

Happy #FluorescenceFriday! Just some random data I collected from a while back, actin and myosin in a U2OS cell imaged on our lattice light sheet microscope.

Ever seen mitochondria fly around like little rockets? 🚀 When cells divide, they don't just split DNA. I made a YouTube story based on a major Nature paper, showing how cells use actin waves to shuffle their powerhouses! 🔬 Watch the video here: youtu.be/udrVh_p7kCg #FluorescenceFriday #Microscopy

✨ Serving egg-cellent organization and fluorescent elegance 🥚 Partial Drosophila 🪰 ovariole with WGA staining of nuclear envelopes 🔬 📸 Image by Michelle Giedt #FluorescenceFriday 🐣

Have a nice #FluorescenceFriday This is an insect larvae with endosymbiotic bacteria enverywhere! (in orange) #Microscopy #Sciart

Captivated by the earliest stage of a fruit fly's life—which completely defies textbook biology—I reached out to the people behind groundbreaking papers. I wove their breathtaking live-cell imaging into a story of self-organization. Super fascinating! youtu.be/xyV9BhIVRjM #Microscopy #CellBiology