Wilson Lab

Wilson Lab

@wilsonlab.bsky.social

Ribosomes & Translation Regulation; Antibiotics & Resistance Mechanisms; Structural Biology & Cryo-EM; University of Hamburg.

604 followers131 following42 posts

Wilson Lab reposted

Israel S. Fernandez
@piratefernandez.bsky.social

Today together with Prof. John McCutcheon lab @arizonastateuni.bsky.social we have visualized by cryoEM what looks like a very funky ribosome from an insect endosymbiont. We believe there are important evolutionary implications. Here the pre-print: www.biorxiv.org/content/10.6...

biorxiv.orgA mitochondrion-like ribosome in an insect endosymbiontUnderstanding how the mitochondrion evolved from a bacterial endosymbiont to an organelle is obscured by time and a lack of comparative examples. It is clear that mitochondrial ribosomes are derived f...

Wilson Lab reposted

Zeynep Baharoglu
@zeynepbaharoglu.bsky.social

EF-P-resolved ribosome stalling and collision trigger Rae1-mediated mRNA decay 🦠https://www.biorxiv.org/content/10.64898/2026.10.09.757949v2 Rae1 cleaves mRNAs with ribosomes stalled on truncated transcripts lacking a stop-codon in B. subtilis

biorxiv.orgEF-P-resolved ribosome stalling and collision trigger Rae1-mediated mRNA decayRibosome stalling compromises proteome integrity and cellular fitness, and requires efficient mechanisms to eliminate problematic mRNAs and rescue blocked ribosomes. We previously identified Rae1 as an endoribonuclease that cleaves mRNAs at the tail end of stalled ribosomes to promote mRNA degradation in Bacillus subtilis. Here, we show that Rae1 has a broader role in the quality control of mRNAs that encounter translation problems. Global 5-end sequencing identified 31 previously unknown Rae1 cleavage sites, many of them associated with ribosome pausing at polyproline motifs in the absence of elongation factor P (EF-P). We also demonstrate that Rae1 cleaves mRNAs with ribosomes stalled on truncated transcripts lacking a stop-codon (non-stop mRNAs), provided that the transcript is sufficiently long to accommodate multiple collided ribosomes. Rae1 cleavage depends on a cytosine-poor context at the cut site, showing that both ribosome collisions and RNA sequence at the cleavage site govern the ability of Rae1 to promote the removal of stalled ribosomes from mRNA. ### Competing Interest Statement The authors have declared no competing interest.

Wilson Lab reposted

Zeynep Baharoglu
@zeynepbaharoglu.bsky.social

The ABC-F protein Uup, Rescues Stalled Ribosomal Termination Complexes to Modulate DNA Maintenance 🦠https://www.biorxiv.org/content/10.64898/2026.10.08.757592v1

biorxiv.orgThe ABC-F protein Uup, Rescues Stalled Ribosomal Termination Complexes to Modulate DNA MaintenanceThe cytosolic ATP-binding cassette F (ABC-F) family modulates ribosomal activity in eukaryotes and prokaryotes. Although they all bind to the E site of the ribosome, some bacterial ABC-Fs confer resis...

Wilson Lab reposted

OMühlemann lab
@omuhlemann-lab.bsky.social

Today, Wojciech Teodorowicz's PhD work on the role of USP9X in occluded A-site RQC was published. Congrats to Wojciech and Lukas, this was not an easy and definitively not a straightforward project. www.cell.com/cell-reports...

cell.comUSP9X promotes the degradation of trapped translation factors on collided ribosomesTeodorowicz et al. show that USP9X, in conjunction with the E3 ligases RNF14 and RNF25, is required to promote the VCP-dependent proteasomal degradation of eRF1 and eEF1A1 trapped in the ribosomal A-site and identify 4EHP as a mediator of translational shutdown following translation factor clearance.

Wilson Lab reposted

Green Lab JHMI
@greenlab.bsky.social

U should check DIS out! In our new paper (and sequel to our RIOK3 story), we find that rRNA oligo(U) tailing and DIS3L2-mediated decay drive 40S ribosome degradation 💫 www.cell.com/molecular-ce...

cell.comOligo(uridine) tailing and exonucleolytic decay drive 40S ribosome degradation in response to stress40S ribosomes are degraded in diverse stress conditions. Diehl et al. find that in response to stress, TUT7 is recruited to ubiquitylated 40S ribosomes and adds 3′ oligo(U) tails to the 18S rRNA. Iter...

Wilson Lab reposted

Nobel Prize
@nobelprize.org

BREAKING NEWS The Royal Swedish Academy of Sciences has decided to award the 2026 #NobelPrize in Chemistry to Henri B. Kagan and Kenso Soai “for the discovery of non-linear effects and autocatalysis in asymmetric organic synthesis.”

Wilson Lab reposted

Nobel Prize
@nobelprize.org

BREAKING NEWS The 2026 Nobel Prize in Physiology or Medicine has been awarded to Karl Deisseroth, Peter Hegemann and Georg Nagel “for their discoveries concerning light-gated ion channels and optogenetics.”

Wilson Lab reposted

Diorge Souza
@diorgeps.bsky.social

Finally out! Our work uncovers evolutionary links between bacterial and archaeal ribosome hibernation and eukaryotic energy sensing, through the archaeal hibernation factor AHA link.springer.com/article/10.1...

link.springer.comA conserved archaeal protein with evolutionary links to bacterial ribosome hibernation and eukaryotic energy sensing - The EMBO JournalRibosome hibernation helps cells survive stress by reversibly silencing translation and preserving ribosomal complexes. Although well characterized in bacteria and eukaryotes, archaeal hibernation rem...

Wilson Lab reposted

Structura Biotechnology
@structurabio.bsky.social

🔎 A classic #cryoEM particle curation conundrum: 2D or 3D classification? "Decoy Classification" using Heterogeneous Refinement in #CryoSPARC curates particles directly in 3D, helping remove junk particles while preserving valuable views. Read more in the CryoSPARC Blog 👉 bit.ly/3SXfwS0

Wilson Lab reposted

Beckmann Lab
@beckmannlab.bsky.social

Frank Nijpels from VitriMatics paid us a visit today. Not only did he repair our broken VitroBot Mark IV and returned it in person, he also brought some extremely cool tools ❄️ for reliable cryo-EM grid freezing 🔬. Check out the VitriClip and the SupraBlot at vitrimatics.com. #cryoEM #cryoET

Wilson Lab reposted

Beckmann Lab
@beckmannlab.bsky.social

New preprint! 👀RNAse activity has to be tightly regulated.✂️🧬Here, we present the dynamic architecture of the conserved Rixosome complex – a major player in ribosome maturation and heterochromatin maintenance. Our results give insights into its mode of activation.👇 #cryoEM #Ribosome #RNA

Wilson Lab reposted

Beckmann Lab
@beckmannlab.bsky.social

Preprint alert: a helicase watching the ribosomal A-site 👀🧬 Our cryo-EM structure reveals how yeast Dhx29 senses mRNA hairpins forming in the A-site and positions its helicase at the mRNA entry channel to resolve them during translation elongation: doi.org/10.64898/202... #cryoEM #ribosome #RNA

Wilson Lab reposted

Stella Hurtley
@smhsci.bsky.social

Out now in @science.org Nonviral delivery of chemically modified tRNA rescues nonsense mutations in cystic fibrosis | Science www.science.org/doi/10.1126/...

science.orgNonviral delivery of chemically modified tRNA rescues nonsense mutations in cystic fibrosisSuppressor transfer RNAs (sup-tRNAs) can rescue disease-causing nonsense mutations by promoting readthrough of premature termination codons (PTCs). Their clinical translation is limited by suboptimal ...

Wilson Lab reposted

Fusion Conferences
@fusionconf.bsky.social

Our speaker line-up is almost complete for #TCCO, do you wish to be a part of it? There are several presentation opportunities available at this meeting, send your abstract to amy@fusion-conferences.com before 19 November to be considered. Sign up for updates here - bit.ly/4yAFomu

Wilson Lab reposted

Chris H. Hill
@chillzaa.bsky.social

Thrilled to share that our paper describing protein-dependent frameshifting is now out in Molecular Cell @cellpress.bsky.social. Huge congratulations to @jemmabetts.bsky.social for publishing the first research paper from her PhD 🧬🧪🥳 www.sciencedirect.com/science/arti... 1/8

sciencedirect.comA protein-dependent riboswitch activates ribosomal frameshifting in cardiovirusesProgrammed −1 ribosomal frameshifting (PRF) is a translational control mechanism used by RNA viruses to regulate the relative abundance of proteins en…

Wilson Lab reposted

Ivana Bukvin
@ivanabukvin.bsky.social

Very happy to share a new preprint characterising the unfolded state of a folding-competent domain at the cusp of folding initiation on the ribosome. Co-led by @julianstreit.bsky.social from my PhD in John Christodoulou's lab. Many thanks to all the co-authors!

biorxiv.orgThe initiation of de novo protein folding on the ribosomeHow the earliest structure within the unfolded state is formed during biosynthesis on the ribosome and whether it has any consequences for downstream folding remain open questions. Here, we combine 15...

Wilson Lab reposted

Florent Waltz
@florentwaltz.bsky.social

This story is now published in @natplants.nature.com ! 🎉🎩 Check out the final article here: nature.com/articles/s41477-026-02361-1 Very similar to the original bioRxiv preprint, with the addition of some cool polysome analysis (in the supplementals)! Thanks and congrats to everyone involved! 🙏

nature.comChloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome - Nature PlantsUsing in situ cryo-electron tomography, researchers found a unique ‘arm’ domain on the small subunit of Chlamydomonas chlororibosomes. This discovery suggests that chlororibosome structure is more evo...
Florent Waltz@florentwaltz.bsky.social

Long in the making, but happy to present the Chlamydomonas chlororibosome! Cryo-ET🔬reveals a large new domain on the small subunit, built from multiple extensions in conserved ribosomal proteins. bioRxiv 📖: shorturl.at/q44tG This suggests greater chlororibosome diversity than expected! 1/n 🧵

Wilson Lab reposted

Nature Plants
@natplants.nature.com

New OA Article: "Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome" rdcu.be/fwoSj In situ cryo-electron tomography reveals a unique ‘arm’ domain on the small subunit of Chlamydomonas chlororibosomes. #PlantScience

Wilson Lab reposted

Zeynep Baharoglu
@zeynepbaharoglu.bsky.social

journals.asm.org/doi/10.1128/... comprehensive review of bacterial RNA modifications 🦠

journals.asm.orgCompendium of RNA modifications for bacterial stress adaptation | Microbiology and Molecular Biology ReviewsSUMMARYRNA modifications in the tRNA, rRNA, and mRNA constitute a widespread layer of post-transcriptional gene regulation, commonly known as the epitranscriptome. In bacteria, tRNA and rRNA modifications are well documented and play essential roles in tRNA folding, rRNA maturation, translation, and peptidyl transfer. Although mRNA modifications have been extensively characterized in eukaryotes, their functional implications for mRNA fate in prokaryotes remain uncertain, and their identities and locations are the subject of an emerging field of research. Importantly, RNA modification is a dynamic process, and variation in the presence and abundance of these modifications has significant consequences for bacterial physiology. Here, we discuss current knowledge of modified RNA nucleotides in bacteria, with a particular focus on their roles in bacterial adaptation to environmental stress.

Wilson Lab reposted

Green Lab JHMI
@greenlab.bsky.social

Wait, what?! This isn’t about ribosomes? Check out our new work where we find that a #Legionella effector thought to target host translation instead targets metabolism by modifying a host glycolytic metabolite. Led by @drjoshblack.bsky.social www.cell.com/molecular-ce...

cell.comThe Legionella pneumophila effector SidL is an adenylyltransferase that modifies the glycolytic intermediate 3-phosphoglycerateBlack et al. identify the L. pneumophila effector SidL as an adenylyltransferase that targets host glycolysis. SidL covalently modifies the glycolytic intermediate 3-phosphoglycerate (3PG) with AMP, g...

Wilson Lab reposted

Kurianlab
@kurianlab.bsky.social

How cool!!

Nicholas Bellono@nbellono.bsky.social

How did octopuses evolve novel traits? Rishav Mitra, Richard Han, Trey Scott, et al serendipitously discover a unique ribosome adaptation that increases the accuracy of protein synthesis and evolved alongside their elaborate nervous systems vs deep sea relatives. www.biorxiv.org/content/10.6...

Wilson Lab reposted

Di Jiang
@dijiang319.bsky.social

In print today @science.org Laser phase plate improves structure determination of small proteins by cryo-EM | Science www.science.org/doi/10.1126/...

science.orgLaser phase plate improves structure determination of small proteins by cryo-EMPhase plates can, in principle, overcome the poor image contrast in cryo–electron microscopy (cryo-EM) and the resulting limits on the structural reconstruction of small proteins. However, previous de...

Wilson Lab reposted

Osterman Ilya
@ostermanilya.bsky.social

Excited to share that our Metis story is now published in Science! 🎉 Bacteria can sense phage-induced degradation of their genome and activate immunity. Many thanks to everyone who contributed to this work. 📄 www.science.org/doi/10.1126/... More details in my earlier thread 🧵⬇️

science.orgBacteria sense virus-induced genome degradation via methylated mononucleotidesPhages often degrade the genome of their bacterial host to individual nucleotides. Here we describe Metis, a bacterial defense system that directly senses phage-mediated host genome degradation. Metis...
Osterman Ilya@ostermanilya.bsky.social

Bacteria can sense when a virus starts shredding their genome — by detecting methylated mononucleotides. Here’s the story of how we discovered the Metis defense system 👇 www.biorxiv.org/content/10.1...

Wilson Lab reposted

Misha Kudryashev
@mishakudryashev.bsky.social

New paper from the group! We were curious if the longevity of naked mole-rats has anything to do with their protein synthesis machinery. It was shown that the ribosomal 28S is split into 3 subunits Our cryo-EM study of the NMR ribosomes by @mehmetguel.bsky.social etal www.nature.com/articles/s41...

nature.comCryo-EM structure of the naked mole-rat ribosome reveals a stabilized split 28S rRNA - Nature CommunicationsThe long-living rodent naked mole-rat has a distinct break in its 28S ribosomal RNA. Here, the authors present cryo-EM structures showing this cleavage is stabilized by surrounding ribosomal proteins,...

Wilson Lab reposted

EMBL
@embl.org

Congratulations, Matthias Hentze! The EMBL Director has been awarded the German Order of Merit for his outstanding achievements in life science research and in building scientific structures across Germany and beyond. www.embl.org/news/awards-...

Wilson Lab reposted

The EMBO Journal
@embojournal.org

Substrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidC @kedrov-lab.bsky.social, Roland Beckmann and coworkers link.springer.com/article/10.1...

link.springer.comSubstrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidC - The EMBO JournalThe Sec translocon and the YidC/Oxa1-type insertases universally mediate biogenesis of α-helical membrane proteins, but the molecular basis of their cooperation has remained disputed. Recent discovery...

Wilson Lab reposted

Kedrov Lab @ HHU
@kedrov-lab.bsky.social

We are on air! Congratulations to everyone behind the story ❤️‍🔥 and thanks to @embojournal.org for providing the stage! link.springer.com/article/10.1...

link.springer.comSubstrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidC - The EMBO JournalThe Sec translocon and the YidC/Oxa1-type insertases universally mediate biogenesis of α-helical membrane proteins, but the molecular basis of their cooperation has remained disputed. Recent discovery...