Charles Bayly-Jones

I’ve officially started my lab at Monash University! 🎉 We’re diving into #cellgrowth and #lysosome biology, through the lens of molecular #structure and mechanism. I’m thrilled to say that PhD student scholarships are available—so please share widely and get in touch if you’re interested. 🤩

Replacing H2O with D2O in cryo-EM reduces radiation damage in biological samples https://www.biorxiv.org/content/10.64898/2026.10.24.761413v1

Anyone else seen this? CryoEM models without any volume reconstruction. Straight from the micrographs, inferred structures restrained by the actual particle data. So sequence + limited particles = structure?! Bypassing all that time making a map just to still model after arxiv.org/html/2610.01...

Happy to share my PhD work on the regulatory mechanism of E3 ligase RNF213! RNF213 is not a monomer, but a dimer that switches between inhibited and activated states in an ATP-dependent manner. We tracked the activation mechanism and showed how disease mutations can destabilize the inhibited state.

Chlamy spotted in the wild @cellarchlab.com

Amazing structure

Nice review covering AFM state-of-the-art

“It is unprecedented. We have two major waterways obstructed, active attacks, Iran escalating attacks, proxies being active and no signs of diplomatic talks. It’s a disastrous situation when you’re looking at it from an energy security angle.” www.nytimes.com/2026/09/16/b...

ColabFold 1.6.3 is out! 2.5x faster, pip-installable, ipSAE+pDockQ2 scores. Thanks Choonghwan Lee, Marielle Russo, Gyuri Kim 🐍pip install colabfold[alphafold] CF2 Sneak Peak with AF3/Boltz/Protenix/ESMFold2… 🐍pip install "colabfold[alphafold3]@git+https://github.com/sokrypton/ColabFold@af3-preview"

New preprint! 👀RNAse activity has to be tightly regulated.✂️🧬Here, we present the dynamic architecture of the conserved Rixosome complex – a major player in ribosome maturation and heterochromatin maintenance. Our results give insights into its mode of activation.👇 #cryoEM #Ribosome #RNA

New online: Cryo-EM structure, enzymatic activity and genome targeting of canonical PRC1

De novo design of autocatalytically forming intra- and intermolecular isopeptide bonds to construct rigid covalent protein assemblies https://www.biorxiv.org/content/10.64898/2026.08.13.744004v1

It’s Census night! For all the unconventional and conventional households out there - let’s stand up and be counted. Every answer matters. Every Australian matters.

Binders for all!

Amazing, well done Bronte!

Take a look at our new paper on the structure of the EHD2 that, upon oligomerization, forms rings that remodel the plasma membrane and form caveolae. Beautiful work by Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff & Oliver Dumke's team; take a look: www.nature.com/articles/s41...

Sometimes I really miss the old Science Twitter that fizzled out around 2023. 🥺

Very excited to see this work, on our insecticidal pore forming protein come out. This was an enormous amount of work and outstanding collaboration between Monash and Cardiff Uni. first put together by Prof Colin Berry. www.nature.com/articles/s41...

#DiscoveryProjects #DP27 Detailed assessor reports should be released in ARC's RMS tomorrow (23 Jun). Normally about 11am, Canberra time (but it varies). Remember the #ARC3DayRule: Think an assessment is "inappropriate"? You've only got 3 days to lodge a "request for review" 👇

Oooooh I'm feeling inspired

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

Well done! Congratulation Jordan! 🍾🎉

1) The Herzik lab is excited to announce our newest preprint in collaboration with the Grotjahn lab! We have developed a surfactant cocktail that seeks to overcome one of the more nefarious problems in #cryoEM, Air-water interface (AWI)-induced damage and preferred orientation (PO).

Sad news coming from our colleagues in the US. A friendly reminder to those who have missed out that we have grad school positions available and we value you! Maybe this is an opportunity to make Melbourne, Australia your new home. Please boost.

FlashPPI is our new proteome-wide protein-protein interaction prediction model. If you haven't had a chance to try FlashPPI yet, here's what the end-to-end flow looks like in SeqHub:

🌀 We solved the structure for T. denticola periplasmic flagella! I'm very happy to finally showcase this work. A huge collaborative effort between myself, @bindusmitapaul.bsky.social, @debnathghosal.bsky.social, Jack Kim, @banfieldlab.bsky.social, Eric Reynolds and the Chris Fenno lab. 🦠 Enjoy!

To learn more, you can find our new preprint here! 13/13 www.biorxiv.org/content/10.6...

We developed a single-molecule, multiplexed, microfluidic force spectroscopy (SM3FS) assay that arrays many flow chambers in one FOV. Additional multiplexing enabled library-scale experiments. 4/13

At #BPS2026? 🧬 Come talk structural biology & mTORC1 signaling! I'll be at Poster B220 this Tuesday, 2:45–3:45 PM. I’m also building the Cui Lab at CU Anschutz and looking for postdocs/students interested in Cryo-EM and ML. Let's chat! 🧪🏔️ #Biophysics #CryoEM #StructuralBiology

www.nature.com/articles/s41...

Introducing AlphaFast 🚀. AF3 is transformative but too slow for large-scale protein design. My students Ben and Jeonghyeon made it 10-100x faster using GPU-accelerated sequence search. Preprint: www.biorxiv.org/content/10.6...  Code: github.com/RomeroLab/al...

New issue alert 👉 www.cell.com/cell/current On the cover, McCallum et al. depict a fluorescent micrograph showing a engineered biosensor strain in the mouse colon, illustrating how commensal bacteria can serve as in situ reporters of dynamic gut physiology.

Tried it. Its awesome. Shame the code isn't available. Webserver only, how long will it last??

I've heard this position articulated by a lot of people, but I think this is hard to make precise. Much of this tracks a problem we already know in science: standards of authorship are not stable and there are many difficult edge cases. Consider these cases...

Physically Grounded Generative Modeling of All-Atom Biomolecular Dynamics www.biorxiv.org/content/10.64898/2026.02.15.705956v1 #cryoEM

Metadynamics meets diffusion-based structure prediction: the same trick that drives enhance sampling of MD trajectories can be adapted and applied as steering terms during generation to sample diverse conformations. Seems obvious in hindsight!

How could a simple self-replicating system emerge at the origins of life? RNA polymerase ribozymes can replicate RNA, but existing ones are so large that their self-replication seems impossible. Could they be smaller? Excited to share our latest work in @science.org on a new small polymerase. 1/n

New online: Structure and function of the yeast amino acid-sensing SEAC–EGOC supercomplex

New preprint from the Robertson Lab! We used AF ensemble generation for rigidity filtering with generative design to make cryoEM fiducials, enabling rapid inactive states of four drug targets and a beta2 extracellular fiducial to study GPCR activation! www.biorxiv.org/content/10.6...

Nature research paper: Construction of complex and diverse DNA sequences using DNA three-way junctions go.nature.com/4qu2ExZ

Not just biomedicine! Chemistry, physics, computer science, climate? Both ARC and NHMRC massively under funded. Success rates abysmal. Skeptical of claim re budget increase by 250%? Aus R&D expenditure (%GDP) is stagnant, significantly below OECD average. Inflation. Rising costs. USA exit. ?!?

First steps toward magnetic-elution affinity chromatographies, easily pattern surfaces, microfluidics, also probably cheaper than mag beads for robotic pipelines. Keen to see the data!

The latest research from our team is out in Cell @cp-cell.bsky.social! We used #cryo-EM, #imaging, #BRET, and #biosensors targeted to the lysosome to understand how the KICSTOR-GATOR1 complex turns off #mTORC1 in response to low levels of amino acids.

Excited to share our latest research, out today in Cell (@cellpress.bsky.social) Using cryo-EM and cell-based studies, we reveal how large nutrient sensing protein complexes form at the lysosomal membrane to turn off mTORC1 and halt anabolic signalling. Read the paper here: doi.org/10.1016/j.ce...

The government can’t claim that medical research is a priority while failing to treat it as one. Nine in 10 leading researchers in Australia are missing out on government support for world‑class proposals, leaving exceptional talent uncertain about their future.

Now online! Structure of the lysosomal KICSTOR-GATOR1-SAMTOR nutrient-sensing supercomplex