Chi-Min Ho

Incredibly proud of Messi Haile @mehsehret.bsky.social, a founding member of the Ho Lab, for her beautiful work uncovering the inner workings of a central piece of the malaria parasite invasion machinery: the malarial moving junction, out today in @cellpress.bsky.social! tinyurl.com/4p3md2eb 🦠❄️🔬

To invade red blood cells, malaria parasites create a temporary molecular portal that lets it slip into the cell — and then disappears behind it. Researchers have finally detailed the proteins that let it break in without tripping the alarms. www.quantamagazine.org/malaria-para...

thrilled to share that my main phd project is finally out in @natcomms.nature.com we present high-resolution structures of RyR1 in native membranes across multiple functional states by both cryo-ET/StA #TeamTomo and SPA! paper: www.nature.com/articles/s41... and a thread below⬇️⬇️⬇️

Cryo-EM peeps are living the dream www.science.org/doi/10.1126/...

Sailing to a deeper understanding of malaria parasite invasion

Millions of beating cilia keep airways clean. At the base of each is the transition zone (TZ), a gate that controls which proteins enter and leave. In our paper out today in Science, we used cryo-FIB-ET to image the TZ inside human airway cells www.science.org/doi/10.1126/...

Mechanism of membrane perforation in rotavirus cell entry | Science www.science.org/doi/10.1126/...

MemBrain v2 is on @natmethods.nature.com now? I never had a @biorxivpreprint.bsky.social with 164 citations before😅 ...Which is also to say: Thank you to the amazing #TeamTomo community, many of whom not only beta tested, but also contributed diverse training data. This one belongs to all of us! 🤗🔬🧪

Check out our preprint on the structural mechanisms of how LEM2 and ESCRTs seal the nuclear envelope. Awesome collaboration with @karenpalacior.bsky.social who brought my structures to life 💃🔬❄️🌀 @hummerlab.bsky.social www.biorxiv.org/content/10.6...

The singular job of all known ribosomes: synthesizing protein. Enter the malaria parasite. In our new work, we show that Plasmodium evolved an entire new kind that does just the opposite - inhibit mRNA translation. www.biorxiv.org/content/10.6...

How do African trypanosomes regulate the complement system? Check out our new study from @BiochemCook, in which he identified a new trypanosome receptor which binds to the core C3bBb convertase of the complement system and blocks its activity. Real all about it! doi.org/10.64898/202...

Thank you @structurabio.bsky.social for the beautiful write-up featuring our recent work on the #CryoSPARC Blog! We are huge fans of both the Blog and the CryoSPARC Case Studies in the @cmholab.bsky.social 🔬❄️🦠❤️

Take a look at our new paper on the structure of the EHD2 that, upon oligomerization, forms rings that remodel the plasma membrane and form caveolae. Beautiful work by Elena, Vasya @vasiliimikirtumov.bsky.social, Jeff & Oliver Dumke's team; take a look: www.nature.com/articles/s41...

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We are excited to share two preprints reporting a mosquito-specific receptor for chikungunya virus and other alphaviruses! 🤩 www.biorxiv.org/content/10.6... www.biorxiv.org/content/10.6... @harvardmicro.bsky.social @harvardvirology.bsky.social @hhmi-science.bsky.social

#mSphere started a new article collections titled mSphere Legacy. These articles will be authored by our senior colleagues in the microbial sciences community to not only reflect back on their own careers and journey's but how they see the future unfolding. @asm.org journals.asm.org/eprint/PZEHP...

Using spIsoNet to address the preferred-orientation problem in cryoEM reconstructions pubmed.ncbi.nlm.nih.gov/42427643/ #cryoEM

New paper from the @xionglab.bsky.social and Mogessie labs at Yale! We uncover the molecular architecture of mammalian oocyte cytoplasmic lattices (CPLs), revealing GTP-bound tubulin associated with a degradative protein complex. Read the paper: doi.org/10.1038/s415...

Now online! Structural basis for host membrane binding and remodeling by invading malaria parasites

#NatMicroPicks Hidden in plain sight! 💊🦠 Assessing overlooked minor compounds uncovered a new antibiotic, manikomycin, from well-studied Streptomyces species that kills bacteria by uniquely targeting the ribosomal E-site #MicroSky www.nature.com/articles/s41...

Now published! We investigated how polyadenylated RNAs are targeted for decay in the human nucleus. www.nature.com/articles/s41... (1/5)

We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇

Nanobodies against malaria parasite adhesins that block receptor engagement and invasion Lovely work by @jaisondsa.bsky.social and Jill Chmielewski on PfRh5, PfRh4 & PvRBP2b nanobodies + stabilized PfRh4 🦙🩸⛰️ Have a read, share widely and please give us feedback! www.biorxiv.org/content/10.6...

Excited to highlight some exciting findings from our recent paper that uncovers how an unusual molecular motor, Myosin F, powers actin dynamics in Toxoplasma gondii. journals.biologists.com/jcs/article/...

Incredibly excited and grateful to be joining the Burroughs Wellcome Fund family! Thank you @bwfund.bsky.social and the selection committee members for supporting and believing in our vision and congrats to the other 2026 PATH Investigators!!! 🥳

The architecture of carriers formed by AP3:ARF1 for lysosomal membrane protein transport: www.science.org/doi/10.1126/.... Work from @jggkaufman.bsky.social, in collaboration with @dgershlick.bsky.social and David Owen. @mpibiochem.bsky.social @thecimr.bsky.social

Absolutely extraordinary work from Hui Gao, John Briggs and co @mpibiochem.bsky.social on the structure of the influenza matrix protein in situ, particularly in filamentous virions. It's beautiful, and answers questions I've been wondering about for twenty years www.biorxiv.org/content/10.6...

Excited about parasites? Love watching movies? Come join our Wellcome-funded project! Parasitology friends, please do share far and wide.

Applications and prospects of cryo-electron tomography in drug discovery and understanding disease Review article by @camilaclemente.bsky.social in our lab www.sciencedirect.com/science/arti...

Thrilled to share the updated video of my paper in Nature with Maciej (under supervision of @simonemattei.bsky.social & @jomaalab.bsky.social) Proud to use Cryo-ET to uncover how hibernation factors are key to translation restart! To model PTMs in situ and render animations fulfilled my PhD dream!🥹🎉

For 40 years the 8-nt ‘Kozak Sequence’ was thought to mark sites of translation initiation. In a new study, we revise this model by identifying an ~80-nt sequence—the extended Translation Initiation Sequence (eTIS)— that guides ribosomes to correct start sites. 🧵 www.biorxiv.org/content/10.6...

Happy to share our preprint! Using massively parallel reporter assays, single-molecule imaging, deep learning and cryo-EM, we decode the sequence requirements for translation initiation and describe the “extended translation initiation sequence” (eTIS) that modulates start codon recognition. 🧵👇

We're super excited to share MissAlignment: a new ML-based approach to reference-free tilt series alignment, spearheaded by @martenchaillet.bsky.social. We think it's going to make your cryo-ET life a lot better. Preprint: www.biorxiv.org/content/10.6... Code: github.com/warpem/miss-... 🧶 Thread:

Big news for correlative cryogenic SMLM/ET! With my colleague Soheil, we (Mahamid and @jonasries.bsky.social labs) indicate lamella-specific illumination can allow 500-1500x higher laser intensities, opening massive new possibilities for correlative imaging #teamtomo www.biorxiv.org/content/10.6...

Can enzymes be made to self-destruct for therapeutic purposes? “Reaction-hijacking of enzymes to generate potent therapeutic modulators in situ”. Very pleased that our Review is part of the 50th anniversary special edition of Trends in Biochemical Science. doi.org/10.1016/j.ti...

Was just asked to spread the word about this position. Structural biology and Chemical Biology positions in Barcelona, one of the great cities of the world! www.irbbarcelona.org/sites/defaul...

I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...

Check out our first lab preprint: A real team effort to understand the Plasmodium GAPM proteins, which form part of a essential bridge between the parasite's cytoskeleton and motility apparatus. Below is a sneak peek of some beautiful microscopy - light and electron! www.biorxiv.org/content/10.6...

Thrilled to share a new collaborative preprint, joint with Sean Prigge @johnshopkinssph.bsky.social, on the apicoplast ACP in #malaria parasites. @uofubiochem.bsky.social @uuhsresearch.bsky.social @parasitesrule.org www.biorxiv.org/content/10.6...

Our paper on the structural and functional basis of the human dimeric OS9–SEL1L–HRD1 ERAD complex, together with @qilabuva.bsky.social, is now out! Huge congratulations to Leo, Emir, and Elina! www.nature.com/articles/s41...

Subcellular Structures in Native Hippocampal Synapses Revealed by Cryo-electron Tomography pubmed.ncbi.nlm.nih.gov/41491937/ #cryoEM

🔬 Our first community-contributed image is here! Stunning SEMs of two Trichomonas vaginalis parasites connected by cytonemes. Want to share your parasite images? Visit parasitesrule.com to contribute! 📸 Salas et al., eLife 2023 doi:10.7554/eLife.86067 @eLife

Happy to share a new preprint exploring the unusual biochemistry of a "weirdly" diverged cytochrome c in malaria parasites that exemplifies a lineage common to many apicomplexan parasites. @uofubiochem.bsky.social @uuhsresearch.bsky.social @uofutahcihd.bsky.social www.biorxiv.org/content/10.6...