Simon Roux

Simon Roux

@simrouxvirus.bsky.social

Virus-obsessed bioinformatician, DOE JGI Scientist, Enjoy exploring the viral world with #metagenomics and other cool #omics toys. He/him. Opinions my own.

4K followers1.9K following252 posts

Simon Roux reposted

doi.orgFrequency-dependent fitness effects are ubiquitous in rapidly adapting microbial populationsIn simple microbial populations, the fitness effects of selected mutations are typically assumed to be constant, regardless of mutant frequency. This assumption underpins predictions about evolutionar...
Joao Ascensao@joaoascensao.bsky.social

How common are frequency dependent fitness effects? New preprint out today 👇 doi.org/10.1101/2025...

doi.orgFrequency-dependent fitness effects are ubiquitousIn simple microbial populations, the fitness effects of most selected mutations are generally taken to be constant, independent of genotype frequency. This assumption underpins predictions about evolutionary dynamics, epistatic interactions, and the maintenance of genetic diversity in populations. Here, we systematically test this assumption using beneficial mutations from early generations of the Escherichia coli Long-Term Evolution Experiment (LTEE). Using flow cytometry-based competition assays, we find that frequency-dependent fitness effects are the norm rather than the exception, occurring in approximately 80\% of strain pairs tested. Most competitions exhibit negative frequency-dependence, where fitness advantages decline as mutant frequency increases. Furthermore, we demonstrate that the strength of frequency-dependence is predictable from invasion fitness measurements, with invasion fitness explaining approximately half of the biological variation in frequency-dependent slopes. Additionally, we observe violations of fitness transitivity in several strain combinations, indicating that competitive relationships cannot always be predicted from fitness relative to a single reference strain alone. Through high-resolution measurements of within-growth cycle dynamics, we show that simple resource competition explains a substantial portion of the frequency-dependence: when faster-growing genotypes dominate populations, they deplete shared resources more rapidly, reducing the time available for fitness differences to accumulate. Our results demonstrate that even in a simple model system designed to minimize ecological complexity, subtle ecological interactions between closely related genotypes create frequency-dependent selection that can fundamentally alter evolutionary dynamics. ### Competing Interest Statement The authors have declared no competing interest.

New pre-print and tool alert ! This one addressing a long-standing viromics issue: we were missing a tool that can reliably assign broad host categories (e.g. prokaryote vs eukaryote) to novel viruses. VHAMSTeR is now available for this thanks to very talented postdoc @susiegriggo.bsky.social !!

Susie Grigson@susiegriggo.bsky.social

🚨 New preprint 🚨 We’ve discovered millions of viruses from sequencing data but we don’t always know who they infect. Our new tool, VHAMSTeR generates broad virus host predictions across the tree of life (prokaryote, plant, animal, fungi and protist hosts) A thread 🧵👇 www.biorxiv.org/content/10.6...

View full thread

One thing I really like is that it automates a lot of analyses that were typically run in an ad hoc way (similarity to known viruses, identification of key features like prokaryotic vs eukaryotic RBS, etc), but this time in a strictly benchmarked and carefully designed framework (with AI !)

New pre-print and tool alert ! This one addressing a long-standing viromics issue: we were missing a tool that can reliably assign broad host categories (e.g. prokaryote vs eukaryote) to novel viruses. VHAMSTeR is now available for this thanks to very talented postdoc @susiegriggo.bsky.social !!

Susie Grigson@susiegriggo.bsky.social

🚨 New preprint 🚨 We’ve discovered millions of viruses from sequencing data but we don’t always know who they infect. Our new tool, VHAMSTeR generates broad virus host predictions across the tree of life (prokaryote, plant, animal, fungi and protist hosts) A thread 🧵👇 www.biorxiv.org/content/10.6...

You will want to check Susie's thread above for all the details about how vHAMSTeR works. My tl;dr version: it is a "first layer" host prediction, and is super useful if you want a big picture of which types of viruses you identified in a given dataset.

Simon Roux reposted

🚨 New preprint 🚨 We’ve discovered millions of viruses from sequencing data but we don’t always know who they infect. Our new tool, VHAMSTeR generates broad virus host predictions across the tree of life (prokaryote, plant, animal, fungi and protist hosts) A thread 🧵👇 www.biorxiv.org/content/10.6...

biorxiv.orgViral host assignment across the tree of life with VHAMSTeRViruses infect organisms across the tree of life, but many are not associated with a specific host. Here we present VHAMSTeR (Virus Host Assignment Model using Sequence Transformers and Reading-frames...

Simon Roux reposted

No smoking in pubs. Craft ale. Wine imports. Computing hardware. More good music. More good films. More good art in general to be honest. Protein structure prediction. Air fryers. Climate change shitshitshitshitshit that wasn’t the brief. Roll that one back. Greggs vegan sausage rolls.

Social media loves talking about how everything is getting worse. But what’s something that you’ve seen get _better_ in your lifetime?

Simon Roux reposted

For this year's #WorldPhageDay we wanted to highlight the historical importance of #phage in Biology So we asked a panel of experts* for their favorite phage-based contributions accross history Here is their top 5 *members of the BBH lab (List of choices was based on doi: 10.1002/bies.201400152)

Simon Roux reposted

Happy World Phage Day to all who celebrate... ... delighted to share new work led by Jacopo Marchi, Chadiga Khalek & joint w/Remy Chait on stable coexistence of phage with migrating bacteria driven by the forces of infection, lysis, and chemotaxis. A 🧵 at the interface between known & unknown.

science.orgStable coexistence and transport of lytic phage infections with migrating bacterial hostsBacteriophages (phages), viruses that exclusively infect bacteria, coexist with their bacterial hosts across diverse environments at densities exceeding 107 per milliliter in marine surface waters, 10...

Simon Roux reposted

Very nice discovery from the Jackson, Bravo and Basel labs. Cleaved tRNA ends, loaded with an amino acid, activate a form of bacterial immunity

bioRxiv Microbiology@biorxiv-microbiol.bsky.social

Aminoacylated tRNA tails trigger auxiliary bacterial immunity https://www.biorxiv.org/content/10.64898/2026.10.14.759216v1

Quick reminder: this is the last day to submit your abstract to VEGA26 if you want it to be considered for a short talk ! (final registration and poster abstract deadline is Nov. 4)

Simon Roux@simrouxvirus.bsky.social

🚨 Registration for VEGA26 is 👏 officially open 👏 🚨 The 5th edition of our Viral EcoGenomics and Applications symposium will be held November 18-19, 2026, on the UC Berkeley campus 🧬 🦠 🔬 🧫 Event website ➡️ jgi.doe.gov/work-with-us... Registration link ➡️ cvent.me/e0RnA0

Simon Roux reposted

I will be advertising a 4-year postdoc soon. We're looking for a molecular biologist with interest in #AMR. You'll join researchers across the Universities of Warwick, Sheffield, Manchester and Leeds to study the emergence of vancomycin resistance in C. difficile. Contact me for more info! #microsky

Simon Roux reposted

Really excited to be co-supervising this PhD project with Spyros Lytras through Institut Pasteur’s PPU programme! Virus evolution × comparative genomics × structural biology Applications are now open (rolling basis), deadline 14 November. Apply: ppu.pasteur.fr

ppu.pasteur.fr Pasteur Paris University International Doctoral Programm
Antigen Evolution & Design Lab@antigenevo.bsky.social

🧑‍🔬PhD Opportunity! Together with @mghafari.bsky.social (Oxford uni) we're advertising an exciting PhD project on exploring the deep evolutionary and structural conservation of viral antigen proteins, through @pasteur.fr 's prestigious PPU program! Apply here: ppu.pasteur.fr

Simon Roux reposted

My weekly reminder that there is still time to register for VEGA26, and come hang out with us to talk about everything virus/phage ecology, genomics, and applications ! And also still time to submit your abstract for poster/short talk, although this deadline is approaching :-)

Simon Roux@simrouxvirus.bsky.social

🚨 Registration for VEGA26 is 👏 officially open 👏 🚨 The 5th edition of our Viral EcoGenomics and Applications symposium will be held November 18-19, 2026, on the UC Berkeley campus 🧬 🦠 🔬 🧫 Event website ➡️ jgi.doe.gov/work-with-us... Registration link ➡️ cvent.me/e0RnA0

Simon Roux reposted

Happy to share that @averynoonan.bsky.social paper “Phylogeny-agnostic strain-level prediction of phage–host interactions from genomes using machine learning” is now published in @natmicrobiol.nature.com 🎉🎊 👉🏽 rdcu.be/nR2NizD7H5va

Avery Noonan@averynoonan.bsky.social

We built GenoPHI: a machine learning workflow that predicts phage-host interactions at strain level. This could help rapidly select phages to treat drug-resistant bacterial infections or for microbiome engineering without exhaustive lab testing. www.biorxiv.org/content/10.1...

Simon Roux reposted

Simon Roux reposted

Exciting news: @uwgenome.bsky.social will be hiring for two tenure-track faculty positions this year, one open rank and one assistant professor. Both are “Genome Sciences” interpreted broadly so we welcome applicants working in a variety of fields and methods. Ad will be out shortly.

Simon Roux reposted

Want to explore host-plasmid and plasmid-plasmid distributions in your dataset? Or any tree with a set of binary traits? I’ve made a CLI for our model here: github.com/wtmatlock/pl...

github.comGitHub - wtmatlock/plasmid-model-cliContribute to wtmatlock/plasmid-model-cli development by creating an account on GitHub.
Will Matlock@wtmatlock.bsky.social

🚨 New preprint 🚨 How promiscuous really are conjugative plasmids, and what does that mean for plasmid co-occurrence? A pleasure to collaborate with @craigmaclean.bsky.social on the first paper of my fellowship! www.biorxiv.org/content/10.6...

Simon Roux reposted

2 more days for VEGA26 early-bird registration ! Get in before it's too late :-)

Simon Roux@simrouxvirus.bsky.social

One week left to catch the early-bird rate for the VEGA26 symposium - Don't miss the deadline, and come share your research and thoughts about virus ecology, virus (meta)genomics, and virus applications ! (also, abstracts for posters and short talks are still open !) jgi.doe.gov/work-with-us...

One week left to catch the early-bird rate for the VEGA26 symposium - Don't miss the deadline, and come share your research and thoughts about virus ecology, virus (meta)genomics, and virus applications ! (also, abstracts for posters and short talks are still open !) jgi.doe.gov/work-with-us...

jgi.doe.govVEGA Symposium | Joint Genome InstituteBerkeley, CA
Simon Roux@simrouxvirus.bsky.social

Quick reminder - VEGA26 early-bird registration rates are on until Sept 19 ! If you are interested in virus/phage-host interaction, functional annotation of novel virus/phage genomes, and microbiome manipulations with virus/phages, you will have an amazing time Nov 18-19 in Berkeley 😁 🧬 🦠 🔬 🧫

Simon Roux reposted

Imagine being responsible for founding and doing the first-ever edit to Wikipedia? Such a flex. 🎥 mister.tomfoolery on TikTok

Simon Roux reposted

Two announcements regarding AccuSNV, which calls high precision SNVs across microbial genomes: Version 1.1 is now incredibly easy to install, run, and perform downstream analyses with (see image of typical output!). The manuscript was also published this summer in Genome Research! Links next...

Simon Roux reposted

Join us and be part of the VirusREvolution! Study the link between the environment and phage host range in soil and groundwater systems 👇👇

Kuesel Lab@kuesellab.bsky.social

We're hiring! Join the Aquatic Geomicrobiology group as a PhD researcher to explore virus–host interactions and host range evolution in soil and groundwater using cultivation experiments, viral tagging & metagenomics. 🦠🧬 📍Jena | 🗓️ Apply by Oct 1, 2026 jobs.uni-jena.de/jobposting/2...

Simon Roux reposted

You can use this UN-approved Equal Earth projection right now in #rstats #gis #databs

CNN@cnn.com

The United Nations General Assembly voted to adopt a new world map that reflects countries’ true relative sizes, after African nations urged the UN to right a centuries-old wrong.

Simon Roux reposted

Quick reminder - VEGA26 early-bird registration rates are on until Sept 19 ! If you are interested in virus/phage-host interaction, functional annotation of novel virus/phage genomes, and microbiome manipulations with virus/phages, you will have an amazing time Nov 18-19 in Berkeley 😁 🧬 🦠 🔬 🧫

Simon Roux@simrouxvirus.bsky.social

🚨 Registration for VEGA26 is 👏 officially open 👏 🚨 The 5th edition of our Viral EcoGenomics and Applications symposium will be held November 18-19, 2026, on the UC Berkeley campus 🧬 🦠 🔬 🧫 Event website ➡️ jgi.doe.gov/work-with-us... Registration link ➡️ cvent.me/e0RnA0

Simon Roux reposted

Quick reminder - VEGA26 early-bird registration rates are on until Sept 19 ! If you are interested in virus/phage-host interaction, functional annotation of novel virus/phage genomes, and microbiome manipulations with virus/phages, you will have an amazing time Nov 18-19 in Berkeley 😁 🧬 🦠 🔬 🧫

Simon Roux@simrouxvirus.bsky.social

🚨 Registration for VEGA26 is 👏 officially open 👏 🚨 The 5th edition of our Viral EcoGenomics and Applications symposium will be held November 18-19, 2026, on the UC Berkeley campus 🧬 🦠 🔬 🧫 Event website ➡️ jgi.doe.gov/work-with-us... Registration link ➡️ cvent.me/e0RnA0

Simon Roux reposted

This job ad is now live! Please share or apply here: jobs.montana.edu/postings/52780 Screening of applicants begins Sept 15, 2026 #MicroSky #PhageSky #ScienceJobs

jobs.montana.eduPostdoctoral Researcher - Lyme Disease Bacteriophage BiologyThe Secor Lab seeks a postdoctoral researcher to investigate bacteriophage biology in the Lyme disease spirochete Borrelia burgdorferi. The successful candidate will help define how phiBB-1 controls h...
Patrick R. Secor@prsecor.bsky.social

I’ll soon be #Hiring a postdoc to join the Secor Lab at Montana State University. This NIH-funded position will investigate phage biology in Lyme disease spirochetes. The official ad will be available soon. Please share with anyone who might be interested! #MicroSky #PhageSky 🔬🦠🧪🧫

Simon Roux reposted