Casper Berger

We developed Adaptive Polishing for the consistent preparation of ~150 nm thin FIB-lamellae. This was done by using imaging feedback to adjust parameters for each lamella, or Adaptive Milling, rather than applying a fixed recipe. Preprint: doi.org/10.64898/202...

Do you find cryoFIB-milling tedious and slow? Would you like the slowest steps to go faster? If the answer to these questions is yes then we have just the solution for you! @ox.ac.uk, @diamondlightsource.bsky.social #cryoET

🔬 I’m hiring a postdoc to join my group at the Rosalind Franklin Institute! Contact me if you’re interested in using cryo-ET to study the molecular organisation of human brain tissue! 📍 Harwell, UK 📅 Deadline: 8 Nov opportunities.rfi.ac.uk/vacancy/rese...

📢 We're hiring! Join the Franklin as a Scientist in Advanced Imaging for Women's Health. Combine cutting-edge imaging, AI and Biology to tackle key challenges in reproductive and maternal health. Find our more and apply here: tinyurl.com/3yasjknn #WomensHealth #Hiring #AdvancedImaging

Applications are open for the final UK Home candidate place in our 2026–2027 PhD cohort. Join our fully funded interdisciplinary PhD programme and help develop new technologies for the life sciences. Learn more and apply: www.rfi.ac.uk/careers-stud... 📅 Deadline: 14th August

Cryo-ET gives unparalleled views of cellular structures, but targeted sample preparation by cryo-FIB milling has been a constraint. No longer! A new workflow yields close to 100% targeting success for centrioles & other small structures. Read the preprint ⤵️ www.biorxiv.org/content/10.6...

Exciting PhD position available in the Diepold lab @kit.edu ! We're especially interested in international students with expertise in molecular biology and ready to lead a cutting-edge research project in molecular machines and infection biology. Find out more at diepoldlab.com.

We're happy to announce our new preprint! 🐸 easymode: general pretrained networks for cellular cryo-ET. Segment ~20 cellular features – ribosomes, microtubules, mitochondria, nuclei & more – with zero model training. 🔗 doi.org/10.64898/202... 🧵👇

Sub-cellular chemical mapping using correlated cryogenic electron and mass spectrometry imaging online @natmethods.nature.com, led by Hannah Ochner, collaboration with @catfranco.bsky.social and @kiranrpatil.bsky.social labs doi.org/10.1038/s415...

MissAlignment is out!!! Our new software that improves reference-free tilt series alignment by mimicking human intuition. Check out Dimitry's beautiful thread for a quick summary! And the preprint for more details: www.biorxiv.org/content/10.6... The program: github.com/warpem/miss-...

Preprint 🧵! I’m really excited to introduce BIGSMALL, an interleaved multi-magnification cryo-ET scheme bridging molecular and cellular scales in a single acquisition! BIGSMALL = 𝐁road 𝐈nformation 𝐆athering 𝐒trategy by 𝐌ultiscale 𝐀cquisition of lame𝐋𝐋a www.biorxiv.org/content/10.6... (1/6)

📢 We’re hiring a Research Software Engineer Work at the intersection of AI, data science, and life sciences—developing software, supporting research teams, and building HPC/cloud infrastructure. Join a collaborative team driving scientific innovation. Apply: opportunities.rfi.ac.uk/vacancy/rese...

I am excited to share our most recent work collaborating with @centriolelab.bsky.social and @stearnslab.bsky.social to look at the ciliary base of mammalian multiciliated cells w/ cryo-ET, XL/MS, and U-ExM www.biorxiv.org/content/10.6...

The newly established NeCEN Utrecht is looking for a #cryoEM scientist! www.uu.nl/en/organisat... Work with a new Krios 5 + pFIB and support cutting-edge life science research. 📍 Utrecht | 🗓 Apply by 30 April Please share with your network!

Hello, world! We are starting a worker cooperative to build, maintain, teach, and support open source scientific imaging software. image.coop/blog/posts/2...

Organelles do NOT have a single uniform pH. And if you think they must, because “protons diffuse fast,” this paper is for you. A thread on why that assumption is wrong; and what we found instead. 🧵 1/n

BBC News - Top UK scientist says research visa restrictions endanger economy www.bbc.com/news/article...

📢 We are on the lookout for Research Associates to support our Multidimensional Imaging Team across several cutting-edge projects in the following areas: • Electron ptychography • Liquid phase electron microscopy • Electron spectroscopy and diffraction Find out more: tinyurl.com/4ce5t7sm

Our latest T7SS study is now out in Science Advances! We solved the cryoEM structure of the T7SSb core unit (T7bCU) composed of YukB, YukC, and YukD from Bacillus subtilis, revealing how these components assemble within the secretion machinery. www.science.org/doi/10.1126/...

What if even the core of bacterial nanomachines wasn’t static? We found that the T3SS core protein SctD in Yersinia dynamically exchanges subunits — and this flexibility is essential for proper assembly & function. 🔗 www.nature.com/articles/s41... @kit.edu @t3sss.bsky.social

Very happy that NWO supports this major upgrade of our national EM infrastructure. It is great that this initiative got broad support all over NL, and it will EMPower fantastic science - looking forward to that! www.uu.nl/en/news/33-m...

Happy to announce "Technical Innovations Using #SerialEM: A Community Meeting", a new format of hybrid meeting David Mastronarde and I are organizing in April 2026. For more info, a preliminary list of speakers, pre-registration etc. visit www.nexperion.net/semcm2026

Political choices have direct effects on #science 🧪 and a cascading impact on society, health, economy, etc. Many are still convinced that science should be apolitical........

In their new study, Beata Turoňová and Tomáš Majtner trained 13 neural networks to automatically detect and remove those corrupted frames, speeding up processing and saving time for tomographers 🥳

And here it is now in print! www.cell.com/cell-reports... With a front cover splash 😍

Proud to share our latest paper. doi.org/10.1016/j.cr... Through the dedication of @glynnca.bsky.social and @cryingem.bsky.social we report a thorough method to image molecular organisation within hippocampus tissue. Structural biology in tissue is well and truly here! @rosfrankinst.bsky.social

We are happy to release LocScale2.0: a tool for context-aware, confidence-weighted cryoEM map optimisation. Taking two half maps as input, LcoScale-2.0 produces feature-enhanced maps along with a robust confidence score that guides objective map interpretation. cryotud.github.io/locscale/

“The Website” is here! It can be intimidating getting into analysis of #CryoET data, and software is evolving all the time👩‍💻🚀. @phaips.vd.st & @florentwaltz.bsky.social put our lab’s workflows into an online guide. We hope this helps lower the bar to joining #TeamTomo. Feedback much appreciated! 🧪🧶🧬🔬

Another super paper came out this week from Amit Kohli and Laura Waller on Ring deconvolution microscopy. This one is fairly technical, but essentially it's a computational approach that lets you do deconvolution when there is a spatially varying PSF. www.nature.com/articles/s41...

Maud Dumoux from RFI tune in on zoom to find out more now! @ccpem.bsky.social @diamondlightsource.bsky.social

A colder frontier in cryo-EM 🧪🔬 Chris Russo’s group, inc. Joshua Dickerson, adapted #cryoEM to work at liquid helium temperatures (13 kelvins), where every frame captured contains more information than the equivalent using liquid nitrogen (81 kelvins). Read more: tinyurl.com/mwwcunkc #LMBResearch

Very compelling study showing the association between herpes zoster vaccination and dementia prevention. The authors leveraged a unique cohort for this natural experiment which shows a reduction in dementia risk of 20% in vaccinated individuals. #MedSky #IDSky 🧪 www.nature.com/articles/s41...

📸 Nadisha Gamage @rosfrankinst.bsky.social took a photo with the Node's polaroid frame during the poster session yesterday #biologists100 The key words of her research are #nucleosomes #cryoEM #stemcells #chromatin #FIBSEM

In a new Science study, cryo–electron tomography captures the in-cell architecture of the mitochondrial respiratory chain, illuminating how the coordinated action of molecular machines drives life’s fundamental energy conversion. Learn more in this week's issue: scim.ag/3FA3Ygq

Automated Removal of Corrupted Tilts in Cryo-Electron Tomography https://www.biorxiv.org/content/10.1101/2025.03.13.642992v1

AreTomoLive: Automated reconstruction of comprehensively-corrected and denoised cryo-electron tomograms in real-time and at high throughput www.biorxiv.org/content/10.1101/2025.03.11.642690v1 #cryoEM

Published in @naturecomms.bsky.social, Franklin researchers describe a high-throughput methodology for cryo focused ion beam lamella preparation. This technique enabled a high-resolution structure (4.0 Å) of an E. coli ribosome using sub volume averaging. Read the full article here: zurl.co/WhpcB

Dr Liang Wu and his team investigate the function of enzymes in heparan sulfate production – long, complex chains of sugar molecules found in every cell of the body. In this latest science update, Liang reflects on how new imaging techniques are helping in this area. Read more here - zurl.co/aB4ks

Check out our paper on Xenon milling of HPF samples, now online @naturecomms.bsky.social rdcu.be/eczIb

In situ and in vitro cryo-EM reveal structures of mycobacterial encapsulin assembly intermediates pubmed.ncbi.nlm.nih.gov/39955411/ #cryoEM