Gerard Lab

Gerard Lab

@gerardlab.bsky.social

We love T cells, anti-tumour immunity and imaging! Lab at the Kennedy Institute of Rheumatology, University of Oxford.

588 followers562 following11 posts

Pinned

Gerard Lab
@gerardlab.bsky.social

Tumours escape the immune system by mutating to decrease their immunogenicity. In this study, now in @natcomms.bsky.social, we described that immune cells can adapt to IFNg-driven escape, enhancing their fitness and thereby counteracting tumour escape www.nature.com/articles/s41...

nature.comRemodelling of the immune landscape by IFNγ counteracts IFNγ-dependent tumour escape in mouse tumour models - Nature CommunicationsLosing sensitivity to IFNγ may contribute to tumor immune escape, but containment of IFNγ-insensitive tumors is also reported. Here the authors show, by analyzing immune landscape differences between ...

Gerard Lab reposted

Oxford Cancer
@oxfordcancer.bsky.social

The DPhil in Cancer Science is inviting PIs from across Oxford University’s medical, physical, engineering, data, and mathematical sciences to submit their project ideas for its 2027 intake! Deadline: 12th June More information on our website ➡️ www.cancer.ox.ac.uk/study @medsci.ox.ac.uk

cancer.ox.ac.ukDPhil in Cancer ScienceWe use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message…
Gerard Lab
@gerardlab.bsky.social

I always struggled to find the OT-I TCR affinity values for the OVA peptide and altered peptides. Here are the updated Kd values! link.springer.com/article/10.1...

link.springer.comMurine T-cell receptor OT-I exhibits imperfect discrimination between foreign and self-antigens - The EMBO JournalT cells use their T-cell receptors (TCRs) to discriminate between higher-affinity foreign and lower-affinity self-peptide-MHC (pMHC) antigen complexes. The OT-I mouse TCR is widely used to study antig...

Gerard Lab reposted

Omer Dushek
@tcell.bsky.social

Our work accurately measuring the 3D affinity between the OT-I TCR and a large number of foreign and self antigen ligands is now published at EMBO Journal. Murine T-cell receptor OT-I exhibits imperfect discrimination between foreign and self-antigens www.embopress.org/doi/full/10....

embopress.orgMurine T-cell receptor OT-I exhibits imperfect discrimination between foreign and self-antigens | The EMBO JournalimageimageThe OT-I mouse T-cell receptor (TCR) is thought to perfectly discriminate between lower-affinity self- and higher-affinity foreign antigen-MHC complexes. This study shows OT-I TCR to exhibit imperfect discrimination and to be activated ...
The EMBO Journal@embojournal.org

Murine T-cell receptor OT-I exhibits imperfect discrimination between foreign and self-antigens Omer Dushek @tcell.bsky.social and colleagues show that 3D solution affinities of the OT-I TCR for foreign and self-antigens predict functional T-cell responses www.embopress.org/doi/full/10....

Gerard Lab reposted

Yang Luo
@y-luo.bsky.social

📣First paper from the lab is out in @science.org‬! 📣https://www.science.org/doi/10.1126/sciadv.adu3461 It’s well known that HLA shapes the TCR repertoire — but how does this change under immune checkpoint blockade (ICB)?

science.orgDefining the genetic determinants of CD8+ T cell receptor repertoire in the context of immune checkpoint blockadeImmune checkpoint blockade influences the genetically determined relationship between TCR repertoire and HLA alleles in cancer.

Gerard Lab reposted

Kennedy Institute of Rheumatology
@kiroxford.bsky.social

Congratulations to Dr Fränze Progatzky, who has been awarded a Lister Institute Research Prize to support her research into glial cell biology! www.kennedy.ox.ac.uk/news/dr-fran... @thelisterinstitute.bsky.social @franzeprogatzky.bsky.social

kennedy.ox.ac.ukDr Fränze Progatzky wins Lister PrizeDr Fränze Progatzky, Principal Investigator in Tissue Biology and Wellcome Career Development Fellow, has been awarded a Lister Institute Research Prize to support her outstanding science.

Gerard Lab reposted

Adrian Liston
@labliston.bsky.social

I'm excited about a new @biorxivpreprint.bsky.social story that just went live! This one takes a #computational #immunology approach to understanding #tissueresident lymphocytes. The story highlights the extra value mathematical modelling can bring to biology. 🧵1/10 www.biorxiv.org/content/10.1...

biorxiv.orgProbabilistic migration events drive transient tissue residency of lymphocytes during homeostasisTissue-resident lymphocytes form a phenotypically and functionally distinct analog to the corresponding circulatory lymphocyte populations. Residential CD8 T cells, in particular, are identified as having prolonged residence in the tissues and key functions in recall responses at tissue-environmental interfaces, although the dwell time in individual tissues has yet to be resolved. Residential CD4 T cells, regulatory T cells, B cells, and NK cells have been demonstrated to share phenotypic properties with residential CD8 T cells, but the migratory kinetics are even more poorly defined. Here we used probabilistic modelling on a large parabiosis dataset, covering multiple time-points and tissues, to calculate migration kinetics and dwell times of multiple lymphocyte subsets across a diverse set of tissues. Markov chain modelling identified distinct cell type-specific and tissue-specific residency patterns. The liver and gut were prone to prolonged residency compared to other tissue types, and a hierarchy of residency was observed with CD8 T cells and NK cells demonstrating longer residency than CD4 conventional T cells and regulatory T cells, which in turn resided in tissues longer than B cells. With few exceptions, however, average residency was at least an order of magnitude shorter than the life-span of the mouse, indicating a more dynamic form of steady-state tissue residency than usually assumed. Together these data provide a comprehensive model of a pan-tissue shared program in lymphocyte tissue residence, as well as identifying cell type- and organ-specific modification of the migratory kinetics. ### Competing Interest Statement The authors have declared no competing interest. Wellcome Trust, https://ror.org/029chgv08, 222442/Z/21/Z European Research Council, TissueTreg

Gerard Lab reposted

Gabriel Victora
@victora.bsky.social

Dear uLIPSTIC users, We have been getting reports that, under some more inflammatory settings like TB or chronic LCMV infection, uLIPSTIC-expressing lymphocytes adoptively transferred into cre-negative uLIPSTIC hosts may be rejected at later timepoints (>1 wk).

Gerard Lab reposted

Tim Elliott
@timmunologist.bsky.social

There are still a few places left for this major @oxfordcancer.bsky.social fixture. We are keeping registration open until places are filled so sign up now!

Oxford Cancer@oxfordcancer.bsky.social

Registration for the Oxford Cancer Immuno-Oncology Network (OCION) Annual Symposium 2025 closes midday 23rd May! Abstract deadline: 15th May. Big thanks to this year's sponsors: @10xgenomics.bsky.social @Immunocore @nanoporetech.com @imtadvances.bsky.social

eventbrite.co.ukOxford Cancer Immuno-Oncology Network (OCION) Annual Symposium 2025The OCION Annual Symposium aims to help catalyse immuno-oncology research across Oxford

Gerard Lab reposted

IMAGE Lab
@labphan.bsky.social

Happy #DayofImmunology! Congrats @ramadhenni.bsky.social & Alexandra Carey Hoppé it's out (finally!) @cp-cell.bsky.social! Incredibly grateful to co-leads @mlmunier.bsky.social, Vanessa Venturi & Tony Kelleher & all brilliant folks @garvaninstitute.bsky.social & Kirby Inst. for fantastic collab! 🧵

sciencedirect.comMacrophages direct location-dependent recall of B cell memory to vaccinationVaccines generate long-lived plasma cells and memory B cells (Bmems) that may re-enter secondary germinal centers (GCs) to further mutate their B cell…

Gerard Lab reposted

fairfaxlab
@bpfairfax.bsky.social

www.nature.com/articles/s41... Proud of my group and collaborators for this work published today in Nature Medicine. We find Cytomegalovirus, which has a profound impact on T cell immunity, is associated with distinct clinical outcomes in patients receiving checkpoint immunotherapy for melanoma.

nature.comCMV serostatus is associated with improved survival and delayed toxicity onset following anti-PD-1 checkpoint blockade - Nature MedicineAnalysis of multiple cohorts of patients with melanoma demonstrates a positive association between cytomegalovirus serostatus and overall survival in patients treated with monotherapy but not combinat...

Gerard Lab reposted

Oxford Cancer
@oxfordcancer.bsky.social

Last chance to apply for our 2025 DPhil in Cancer Science Programme! 🥼 We are open to applications from Medical Undergraduates 📚 We also have 3 GSK-Oxford DPhil studentships available. 📢 Deadline: Noon 25th April. Apply below ⬇️ @medsci.ox.ac.uk www.cancer.ox.ac.uk/study#how-to...

cancer.ox.ac.ukDPhil in Cancer ScienceWe use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message…

Gerard Lab reposted

Hallou Lab
@halloulab.bsky.social

🚨 Paper Alert 🚨 Absolutely delighted to share our last paper on "spatial mechano-transcriptomics" @naturemethods👇!! www.nature.com/articles/s41...

nature.comA computational pipeline for spatial mechano-transcriptomics - Nature MethodsThe authors present a computational framework that leverages mechanical force inference and spatial transcriptomics to enable analyses of the interplay between the transcriptomic and mechanical state.

Gerard Lab reposted

JelenaBezbradicaLab
@jelenalab.bsky.social

The loss of TBK1, or both TBK1 and the related kinase IKKε, results in uncontrolled cell death–driven inflammation. We show that TBK1/IKKε prevent premature cell death by limiting the activity of multiple death pathways in myeloid cells. www.science.org/doi/10.1126/...

science.orgTBK1 and IKKε prevent premature cell death by limiting the activity of both RIPK1 and NLRP3 death pathwaysTBK1 and IKKe prevent premature cell death by limiting the activity of multiple death pathways in myeloid cells.

Gerard Lab reposted

Mauro Gaya
@maurogaya.bsky.social

🦠🔬 We are very excited to organize an EMBO workshop on Immunity in Barrier Tissues with Carolyn King! @immunologyking.bsky.social 📍 Where: Basel, Switzerland 📅 When: 26th–29th August 2025 📝 Registration deadline: 30th May 2025 Check out the program and details 🔗 #EMBOBarrierTissues @embo.org

meetings.embo.orgAdaptive immunity in barrier tissuesImmune responses lie at the heart of almost every aspect of human health, including host responses to infection, autoimmunity, cancer, metabolism and aging. While adaptive immune responses by B and T…

Gerard Lab reposted

Fränze Progatzky
@franzeprogatzky.bsky.social

🚨 We have TWO exciting open positions in our lab! 🚨 We are looking for talented, highly motivated, and collaborative individuals to join our dynamic team as: 🔬 Research Assistant www.kennedy.ox.ac.uk/jobs/researc... 💻 Bioinformatics Postdoc www.kennedy.ox.ac.uk/jobs/bioinfo... 📢 Please share!

kennedy.ox.ac.ukResearch Assistant (177885)We are looking for a Research Assistant with a background in immunology/neurobiology to join the interdisciplinary laboratory of Dr Fränze Progatzky at the Kennedy Institute.

Gerard Lab reposted

Miguel Reina-Campos
@reinacampos.bsky.social

It turns out the immune system is playing 4D chess while we thought it was just playing checkers. Using #spatialtranscriptomics from @10xgenomics.bsky.social and @vizgen.bsky.social we were able to peek into the secret lives of tissue resident T cells in the gut as they form after infection 1/n

nature.comTissue-resident memory CD8 T cell diversity is spatiotemporally imprinted - NatureProfiling of the location and transcriptome of tissue-resident memory CD8 T cell formation at single-transcript resolution finds regionalized signalling as the basis of immune diversity in t...
Gerard Lab
@gerardlab.bsky.social

The OT-I TCR is often criticised as being very high-affinity and displaying unusual behaviour compared to other TCR transgenics. New work from @tcell.bsky.social shows this might not be the case. Will I still use OT-I and all the wonderful tools associated with it? Yes I will!

Omer Dushek@tcell.bsky.social

Our new work! The 3D affinities of the OT-I TCR to foreign and self-antigens predict their 2D affinities and reveal imperfect antigen discrimination www.biorxiv.org/content/10.1...

Gerard Lab reposted

Joshua Bull
@joshuabull.bsky.social

FYI for anyone interested in MuSpAn - we've updated the preprint this week with the full SI. Now featuring more examples of how various different spatial metrics can be strung together in order to make spatial pipelines focussed on specific biological questions! www.biorxiv.org/content/10.1...

biorxiv.orgMuSpAn: A Toolbox for Multiscale Spatial AnalysisThe generation of spatial data in biology has been transformed by multiplex imaging and spatial-omics technologies, such as single cell spatial transcriptomics. These approaches permit a detailed mapp...

Gerard Lab reposted

Daniel Utzschneider
@danielutzschneider.bsky.social

Two studies published in @nature.com today showing that Tpex-like cells develop independent from acute or chronic infection #Tpex www.nature.com/articles/s41...

nature.comPrecursors of exhausted T cells are preemptively formed in acute infection - NatureNature - Precursors of exhausted T cells are preemptively formed in acute infection
Nature [Unofficial]@nature.com.web.brid.gy

nature.comAn early precursor CD8 T cell that adapts to acute or chronic viral infectionNature, Published online: 08 January 2025; doi:10.1038/s41586-024-08562-y An early precursor CD8 T cell that adapts to acute or chronic viral infection

Gerard Lab reposted

Joshua Bull
@joshuabull.bsky.social

Very pleased to announce that our package for Multiscale Spatial Analysis - MuSpAn - has landed! The preprint is on biorxiv now, but you can find a lot more details at www.muspan.co.uk, including documentation, worked tutorials (an ever-expanding list), install guides, and how to get the code!

muspan.co.ukMuSpAn: a Multiscale Spatial Analysis toolbox for biologists | MuSpAn: a Multiscale Spatial Analysis toolboxMuspan is a powerful software package designed for biologists to perform spatial analysis on imaging data. Our multiscale toolbox simplifies the analysis of spatial transcriptomics and histology, enab...
bioRxiv Cell Biology@biorxiv-cellbio.bsky.social

MuSpAn: A Toolbox for Multiscale Spatial Analysis https://www.biorxiv.org/content/10.1101/2024.12.06.627195v1

Gerard Lab reposted

Max Krummel
@maxkrummel.bsky.social

Many tx studies (Mujal... Azizi…)failed to validate M1/M2 signatures in vivo. Many also suspected that calling cells bearing ‘prototypical M2’ genes ‘‘immunosuppressive’ was simplistic + incorrect. Nonetheless many (us too!) leaned on the simplification. Arja addresses the reality head on…thnx JEM

Waggoner Lab@labwaggoner.bsky.social

Rather than immune suppression, CD206+ TAMs recruit a tumor-reactive network of CD8 T cells, cDC1s, and NK cells doi.org/10.1084/jem.... @maxkrummel.bsky.social @jexpmed.bsky.social

Gerard Lab reposted

Ivan Zanoni
@lozanzi.bsky.social

#InterferonPower! Elewaut, @obenaufa.bsky.social &co show @ Nature that inflammatory monocytes can acquire MHC-class I from tumor cells & locally activate CD8 T cells, given that the capacity of the tumor to shut down type I #IFNs & upreg PGE2 is reverted! www.nature.com/articles/s41...

nature.comCancer cells impair monocyte-mediated T cell stimulation to evade immunity - NatureInflammatory monocytes are identified as important players in T cell restimulation in the tumour microenvironment.

Gerard Lab reposted

Paul Thomas
@pgtimmune.bsky.social

We keep improving TIRTL-seq, see the updated preprint (www.biorxiv.org/content/10.1...)! New additions include: a manual 96-well protocol, code and raw data now available, and successful in vitro validation of EBV and SARS-CoV-2 TCRs.

biorxiv.orgTIRTL-seq: Deep, quantitative, and affordable paired TCR repertoire sequencingα/β T cells are key players in adaptive immunity. The specificity of T cells is determined by the sequences of the hypervariable T cell receptor (TCR) α and β chains. Although bulk TCR sequencing offe...

Gerard Lab reposted

Bernard A. Fox
@bernardafox.bsky.social

Underscoring the complexity of #anticancer immunity, Dr Fesneau in the Duhen lab @ #EACRI showed that #IL-12, while driving CD8 T cells to CTL, also drives up-regulation of #immunoregulatory #NKG2A on human #tumor-reactive #CD8. rdcu.be/d0A7V. Very nice work by a great group #FinishCancer

rdcu.beIL-12 drives the expression of the inhibitory receptor NKG2A on human tumor-reactive CD8 T cellsNature Communications - Effective strategies to enhance T cell anti-tumor cytotoxicity are pivotal to improve treatment outcomes. By analyzing tumor samples from patients with head and neck or...

Gerard Lab reposted

Barral Lab
@barrallab.bsky.social

Our first 🦋 post! Very happy to share our latest paper just out in Cell Reports, exploring the crosstalk between immune and epithelial cells in the gut. Check it out! @vivianlilab.bsky.social @kingscollegelondon.bsky.social www.cell.com/cell-reports...

cell.comIFN-γ-dependent regulation of intestinal epithelial homeostasis by NKT cellsThe crosstalk between immune and epithelial cells is vital to maintain intestinal homeostasis. Lebrusant-Fernandez et al. identify NKT cells in the small intestine as key regulators of epithelial biol...

Gerard Lab reposted

Klaus Okkenhaug
@klausokken.bsky.social

Listen to the interview with my brilliant colleague Rahul Roychoudhuri @roychoudhurilab.bsky.social on cancer immunotherapy, synthetic biology, the metastatic niche. And building garden rooms. Starts about 30 min into the podcast.

immunologypodcast.comEp. 93: "Cancer Immune Regulation" Featuring Dr. Rahul Roychoudhuri - The Immunology PodcastDr. Rahul Roychoudhuri is a Professor of Cancer Immunology at the University of Cambridge (Department of Pathology) and Director (Non-Clinical) of the CRUK Cambridge Centre Training Programme. His lab...