Basil Greber

Basil Greber

@bjgreber.bsky.social

Structural biologist. High-resolution imaging of macromolecular assemblies (and landscapes). MRC career development fellow/group leader at the Institute of Cancer Research, London. Views my own.

2.6K followers562 following1.3K posts

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Carl T. Bergstrom@carlbergstrom.com

Your monthly reminder that if you're using ChatGPT to do your science, you're failing your colleagues, community, and all of us. Here, ChatGPT makes up data and presents it as the real thing — then apologizes when confronted.

Waking up to unexpected good news is nice.

Eduardo Amorim@cegamorim.bsky.social

Despite illegal US intervention, manipulation of Meta’s algorithms to favor Bolsonaro Jr., opposition from Brazil’s largest television network, and the politicization of Brazil’s electoral court, the impossible happened. LULA, PRESIDENT OF BRAZIL! 🇧🇷 Against all odds, democracy prevailed.

Basil Greber reposted

I just want to name that people overcame this. Even against a stacked deck, the good fight is worth it. Win or lose, it's worth it. And sometimes the good guys win.

Laura Bassett@lebassett.bsky.social

Bolsonaro-friendly mayors appear to be sabotaging public transport on Election Day. Buses aren’t running in Lula-friendly areas, flights and ferries mysteriously canceled. Traffic lights have gone out and people’s power cut in Salvador, indigenous people’s travel restricted

Basil Greber reposted

Basil Greber reposted

It would be nice if the most powerful man in the world wasn't a delusional lunatic.

Sam Freedman@samfr.bsky.social

New post from @ldfreedman.bsky.social Trump has again intervened in the Ukraine war on the wrong side. How much difference is it making? What can Europe and Ukraine do to counteract? samf.substack.com/p/ukraine-wi...

Basil Greber reposted

Basil Greber reposted

Our work out today in @nature.com! We identified a previously unknown function for WEE1 in cell cycle control that could explain some of the inhibitor side-effects. We found WEE1 inhibits APC/C(Cdh1) to promote S-phase and cell cycle entry. Inhibit Wee1 , block or impair cell cycle entry.

Promising cancer drugs called Wee1 inhibitors have been limited by serious side effects. Researchers have discovered a previously unknown role for Wee1 that could explain why. The findings could help identify patients most likely to benefit & support development of safer, more targeted treatments.

Our plus/minus cryo-tide CDK11-cyclin L-SAP30BP #cryoEM reconstructions are now publicly available: www.emdataresource.org/EMD-59987 www.emdataresource.org/EMD-59992

Basil Greber@bjgreber.bsky.social

During this time, @amymcgeoch.bsky.social in my lab started working with the CDK11 complex, which showed strong preferred orientation. Addition of the original (BH) cryo-tide induced additional particle views, which eventually helped us determine the 2.3 Å-resolution structure of the complex.

Basil Greber reposted

Innocent people were killed by a belligerent foe of the United States because of this choice made by the President and the richest man in the world. It is gross failure and they should both be stripped of all power.

Carl Quintanilla@carlquintanilla.bsky.social

Three-byline alert: 🚨 “.. Last week, Musk capped the speeds at which Ukrainian hardware could stay connected to the satellite-based network .. .. One of the people also claimed Musk and Trump discussed the matter prior to the decision ..” @wsj.com www.wsj.com/world/ukrain...

Basil Greber reposted

Together with other scientists I’m suing the 🇺🇸 US National Institutes of Health for violating our 1st Amendment rights & censoring our health science. I’m ready to shine a light on the darkest days. Buckle up, read on. This gets pretty wild… THREAD

ACLU@aclu.org

By terminating research grants like Kelton's to censor views it doesn’t like, the NIH is blocking scientists from work that would advance public health for all of us. We're pushing back. Tell Congress to demand the NIH restore the grants and stop its censorship campaign at aclu.org/nih_censorship_

Ada Yonath made enormous contributions to the methods of structural biology and to our understanding of the ribosome. It is truly disappointing that this piece contains so many errors. I have written to the Guardian about this.

Basil Greber reposted

Nowhere in that long, rambling word salad would you ever get any inkling, if you didn’t know it, that Putin attacked and invaded Ukraine unprovoked & could end the war at any instant simply by… not doing that anymore.

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Basil Greber reposted

Basil Greber reposted

We recently had one of the most renowned & decorated US researchers present to us by zoom. They requested we not, as we usually would, record the session. This was so they could speak freely about things like climate change without fear of funding cuts, sth they gave examples of happening to peers🫥

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I knew it was time to bring out the good stuff when my phone camera started capturing odd colours in the sky: A few shots from last Saturday's #aurora extravaganza in the UK.

Basil Greber reposted

Was just on Threads and this was my "For you" algorithm when I posted a comment debunking the story. Say what you will about BlueSky being a bubble, but its not designed to enrage and deceive its users

Basil Greber reposted

Selling out Ukraine to side with Russia in order to marginally reduce the negative impacts of his dumb war with Iran

Kyle Cheney@kyledcheney.bsky.social

The United States is now importing fuel from Russia, per President Trump.

Basil Greber reposted

Also happened when I applied for faculty positions in Europe, and as far as I can tell it still happens.

You may be horrified to learn that at Monash until recently (not sure if still true or not) one of the measures was, I shit you not, “Cumulative Impact Factor”, which is the *sum* of the IFs of the journal of each of your papers.

Basil Greber reposted

👇👇👇👇👇👇👇👇

Peggy Stuart@peggystuart.bsky.social

I wish our government would go after pedophiles like they go after brown men headed to work at 7 am. I wish they would close billionaire tax loopholes like they close our public schools. I wish they would fund healthcare like they fund forever wars in the Middle East.  Quotes from James Talarico.

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cryoEM papers@cryoempapers.bsky.social

Cryo-tides: synthetic peptides for cryo-EM grid preparation of multi-subunit protein complexes sensitive to the air-water interface www.biorxiv.org/content/10.64898/2026.09.30.755610v1 #cryoEM

Those who had a careful look at the methods section of our work on the #cryoEM structure of the CDK11-cyclin L-SAP30BP complex will have noticed that we added a short peptide to our sample before freezing. If you wonder why...

Basil Greber@bjgreber.bsky.social

Have a look at our latest results on #CDK regulation, just posted as a pre-print! This study was spearheaded by PhD student Amy McGeoch. Amy has determined the #cryoEM structure of the CDK11-cyclin L-SAP30B complex, an important regulator of #spliceosome activation. www.biorxiv.org/content/10.6...

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We do not claim that cryo-tides will solve all your #cryoEM sample prep issues, but it certainly helped us in several instances. The advantage of this approach is that it is trivial to obtain the peptides, they induce negligible background and are compatible with small targets...

... and they do not reduce particle numbers in the way many detergents do. Have a look at our examples, and give it a shot if you think it might help with your specimens!

Those who had a careful look at the methods section of our work on the #cryoEM structure of the CDK11-cyclin L-SAP30BP complex will have noticed that we added a short peptide to our sample before freezing. If you wonder why...

Basil Greber@bjgreber.bsky.social

Have a look at our latest results on #CDK regulation, just posted as a pre-print! This study was spearheaded by PhD student Amy McGeoch. Amy has determined the #cryoEM structure of the CDK11-cyclin L-SAP30B complex, an important regulator of #spliceosome activation. www.biorxiv.org/content/10.6...

View full thread

During this time, @amymcgeoch.bsky.social in my lab started working with the CDK11 complex, which showed strong preferred orientation. Addition of the original (BH) cryo-tide induced additional particle views, which eventually helped us determine the 2.3 Å-resolution structure of the complex.

Those who had a careful look at the methods section of our work on the #cryoEM structure of the CDK11-cyclin L-SAP30BP complex will have noticed that we added a short peptide to our sample before freezing. If you wonder why...

Basil Greber@bjgreber.bsky.social

Have a look at our latest results on #CDK regulation, just posted as a pre-print! This study was spearheaded by PhD student Amy McGeoch. Amy has determined the #cryoEM structure of the CDK11-cyclin L-SAP30B complex, an important regulator of #spliceosome activation. www.biorxiv.org/content/10.6...

We show that the addition of synthetic peptides - which we refer to as cryo-tides - to #cryoEM samples can protect them from denaturation and can mitigate preferred orientation issues.

Basil Greber reposted

"An ambulance was seen arriving at the prison where Pike was to be executed and then leaving. Medical aid was being administered, according to online federal court records." The utter absurdity of the state trying twice to kill someone and then calling an ambulance. We must end the death penalty.

nbcnews.comTennessee inmate still alive after being administered two lethal injections, her lawyer saysChrista Pike, 50, was 18 when she murdered a classmate. She would be the first woman the state has executed in more than 200 years.

Basil Greber reposted

I spoke with @nature.com about the Nikon Small World controversy. As AI becomes part of scientific imaging, we need greater transparency about what was measured, inferred or generated. It's not about excluding AI. It’s about preserving trust in scientific evidence. www.nature.com/articles/d41...

nature.comThis award-winning microscopy image used AI — igniting controversy in a prestigious competitionResearchers say that using artificial-intelligence tools to visualize scientific images can become problematic when models misrepresent the underlying data.

This further highlights the risks of using tools that were created by companies who were, well, "not very selective" with how they sourced the information required to develop their models. It is becoming increasingly unlikely that users of these tools are being treated any differently.

Carl Zimmer@carlzimmer.com

Four days ago, a scientist was surprised by the news that Anthropic discovered new virus genes for making DNA. He says he’s been studying them for years—and feeding his data to Anthropic’s AI as part of his own research. Coincidence? Here’s my story. nyti.ms/3VSWayc

Basil Greber reposted

Replying to @c0nc0rdance.bsky.social

The obvious conclusion (to me): if you have proprietary research & you upload it into the Plagiarism Nexus, it's not YOUR proprietary research anymore. Behave accordingly.

Basil Greber reposted

Ignore for a moment the question about whether Anthropic trains on user sessions. I am I right in reading this as saying that Anthropic's "never-before-discovered" reverse transcriptase was *patented* three years ago?

Carl Zimmer@carlzimmer.com

Four days ago, a scientist was surprised by the news that Anthropic discovered new virus genes for making DNA. He says he’s been studying them for years—and feeding his data to Anthropic’s AI as part of his own research. Coincidence? Here’s my story. nyti.ms/3VSWayc

Basil Greber reposted

I think it is very important that Big Balls, personally, goes to jail there are a lot of young goons who think they are invincible and it is important to make it clear that they are not